What are the reagents that are used for identify- ing the first amino acid in a protein?

What are the reagents that are used for identify-

ing the first amino acid in a protein?
by

1 Answer

Answer :

Fluoro dinitro benzene, dansyl chloride, phenyl

iso thio cyanate.
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Statement I: In strongly acidic solutions , anline becomes more reactive towards electrophilic reagents Statement II: The amino group being completely

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‘Burn ing foot syndrome’ has been ascribed to the deficiency of (A) Pantothenic acid (B) Thiamin (C) Cobalamin (D) Pyridoxine

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Protein Chemistry (sequencing) vs. Application of Reagents:

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Last Answer : Protein Chemistry (sequencing) vs. Application of Reagents: A simple chemical applied according to a well recognized concept can be responsible for a great advance in biological chemistry. Thus, ... participation into selective, limited, non-enzymatic cleavage at methionine in a peptide chain. 

How many high-energy phosphate bond equivalents are required for amino acid activation in protein synthesis? (A) One (B) Two (C) Three (D) Four

Description : How many high-energy phosphate bond equivalents are required for amino acid activation in protein synthesis? (A) One (B) Two (C) Three (D) Four

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In E. coli the chain initiating amino acid in protein synthesis is (A) N-formyl methionine(B) Methionine (C) Serine (D) Cysteine

Description : In E. coli the chain initiating amino acid in protein synthesis is (A) N-formyl methionine(B) Methionine (C) Serine (D) Cysteine

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Nonsense codons bring about (A) Amino acid activation (B) Initiation of protein synthesis (C) Termination of protein synthesis (D) Elongation of polypeptide chains

Description : Nonsense codons bring about (A) Amino acid activation (B) Initiation of protein synthesis (C) Termination of protein synthesis (D) Elongation of polypeptide chains

Last Answer : Answer : C

Insertion of a base in a gene can cause (A) Change in reading frame (B) Garbled amino acid sequence in the encoded protein (C) Premature termination of translation (D) All of these

Description : Insertion of a base in a gene can cause (A) Change in reading frame (B) Garbled amino acid sequence in the encoded protein (C) Premature termination of translation (D) All of these

Last Answer : Answer : D

Amino acid sequence of the encoded protein is not changed in (A) Silent mutation (B) Acceptable mis-sense mutation (C) Both (A) and (B) (D) None of these

Description : Amino acid sequence of the encoded protein is not changed in (A) Silent mutation (B) Acceptable mis-sense mutation (C) Both (A) and (B) (D) None of these

Last Answer : Answer : A

The half-life of a protein depends upon its (A) Signal sequence (B) N-terminus amino acid (C) C-terminus amino acid (D) Prosthetic group

Description : The half-life of a protein depends upon its (A) Signal sequence (B) N-terminus amino acid (C) C-terminus amino acid (D) Prosthetic group

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Initiation of protein synthesis begins with binding of (A) 40S ribosomal unit on mRNA (B) 60S ribosomal unit (C) Charging of tRNA with specific amino acid (D) Attachment of aminoacyl tRNA on mRNA

Description : Initiation of protein synthesis begins with binding of (A) 40S ribosomal unit on mRNA (B) 60S ribosomal unit (C) Charging of tRNA with specific amino acid (D) Attachment of aminoacyl tRNA on mRNA

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A non essential amino acid is not (A) Absorbed in the intestines (B) Required in the diet (C) Incorporated into the protein (D) Metabolized by the body

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The activity of mammalian L-amino acid oxidase, an FMN – flavo protein, is quite (A) Slow (B) Rapid (C) Both (A) and (B) (D) None of these

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Last Answer : Answer : A

One of the given example is an amino acid: (A) Oh-Lysine (B) Protein (C) Leucine (D) Serine

Description : One of the given example is an amino acid: (A) Oh-Lysine (B) Protein (C) Leucine (D) Serine

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The greatest buffering capacity at physiologic pH would be provided by a protein rich in which of the following amino acids ? (A) Lysine (B) Histidine (C) Aspartic acid (D) Valine

Description : The greatest buffering capacity at physiologic pH would be provided by a protein rich in which of the following amino acids ? (A) Lysine (B) Histidine (C) Aspartic acid (D) Valine

Last Answer : Answer : B

Non-Protein amino acids are (A) Ornithine (B) β-alanine (C) γ-amino butyric acid (D) All of these

Description : Non-Protein amino acids are (A) Ornithine (B) β-alanine (C) γ-amino butyric acid (D) All of these

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Edman’s reaction can be used to (A) Determine the number of tyrosine residues in a protein (B) Determine the number of aromatic amino acid residues in a protein (C) Determine the amino acid sequence of a protein (D) Hydrolyse the peptide bonds in a protein

Description : Edman’s reaction can be used to (A) Determine the number of tyrosine residues in a protein (B) Determine the number of aromatic amino acid residues in a protein (C) Determine the amino acid sequence of a protein (D) Hydrolyse the peptide bonds in a protein

Last Answer : Answer : C

The end product of protein digestion in G.I.T. is (A) Dipeptide (B) Tripeptide (C) Polypeptide (D) Amino acid

Description : The end product of protein digestion in G.I.T. is (A) Dipeptide (B) Tripeptide (C) Polypeptide (D) Amino acid

Last Answer : Answer : D

The amino acid from which synthesis of the protein of hair keratin takes place is (A) Alanine (B) Methionine (C) Proline (D) Hydroxyproline

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Amino acid sequence directs protein folding?

Description : Insulin possesses two polypeptide chains denoted A and B that are linked by disulfide bonds. Upon denaturation by reduction of the SH groups of insulin, followed by reoxidation, only 7% of the ... can these data be reconciled with the hypothesis that the amino acid sequence directs protein folding?

Last Answer : Insulin is synthesized as preproinsulin that is proteolytically processed in the β cells of the islets of Langerhans in the pancreas, to give proinsulin. After synthesis and folding, a section of the ... lacking the C peptide, lacks some of the information necessary to direct the folding process. 

Which amino acid is synthesised after it gets in- corporated into the protein?

Description : Which amino acid is synthesised after it gets in- corporated into the protein?

Last Answer : Hydroxyproline

Addition polymerisation takes place either by a free radical mechanism or ionic mechanism depending on the reagents used. Free radical polymerisation is catalyzed by __________, which decompose to give free radicals. (A) Organic peroxides (B) Sulphuric acid (C) Hydrofluoric acid (D) None of these

Description : Addition polymerisation takes place either by a free radical mechanism or ionic mechanism depending on the reagents used. Free radical polymerisation is catalyzed by __________, which decompose to give free radicals. (A) Organic peroxides (B) Sulphuric acid (C) Hydrofluoric acid (D) None of these

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. Which of the following reagents will react with furan to form 2-furansulfonic acid? (a) SO3 in pyridine at 100°C (b) Dilute H2SO4 at 200°C (c) SO2 at 100°C (d) Dilute H2SO4 at 100°C

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Last Answer : SO3 in pyridine at 100°C

Which of the following reagents does not react with aniline? (a) Acetyl chloride (b) Acetic anhydride (c) Ammonia (d) Nitrous acid

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Which of the following reagents is most suitable for preparing chlorobenzene from benzene? (a) Aqueous chlorine (b) Chlorine in the presence of ultraviolet light (c) Chlorine in acetic acid (d) Chlorine in the presence of FeCl3

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Which of the following reagents will convert acetic acid into acetyl chloride? (a) NaCl (b) HCl/ZnCl2 (c) SOCl2 (d) HCl

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An examp le of group transferr ing coenzyme is (A) NAD+ (B) NADP+ (C) FAD (D) CoA

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Character ist ic f ind ing in Gaucher ’s disease is (A) Night blindness (B) Renal failure (C) Hepatosplenomegaly (D) Deafness

Description : Character ist ic f ind ing in Gaucher ’s disease is (A) Night blindness (B) Renal failure (C) Hepatosplenomegaly (D) Deafness

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The repeat ing d isacchar ide un it in celluslose is (A) Sucrose (B) Maltose (C) Dextrose (D) Cellobiose

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After re leas ing O2 at the tissues, hemoglobin transports (A) CO2 and protons to the lungs (B) O2 to the lungs (C) CO2 and protons to the tissue (D) Nutrients

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The fo l low ing substances are ce l l inclusions except (A) Melanin (B) Glycogen (C) Lipids (D) Centrosome

Description : The fo l low ing substances are ce l l inclusions except (A) Melanin (B) Glycogen (C) Lipids (D) Centrosome

Last Answer : D

Sour taste of lemon is due to the presence of which of the follow-ing? (1) Citric acid (2) Acetic acid (3) Oxalic acid (4) Formic acid

Description : Sour taste of lemon is due to the presence of which of the follow-ing? (1) Citric acid (2) Acetic acid (3) Oxalic acid (4) Formic acid

Last Answer : (1) Citric acid Explanation: Lemon juice is about 5% citric acid, which gives lemons a sour taste and a pH of 2 to 3. Citric acid is found in many fruits but lemon contains large quantity of ... of lemon juice makes it a key ingredient in drinks and foods such as lemonade and lemon meringue pie.

Which acid is used in Soap manufactur- ing?

Description : Which acid is used in Soap manufactur- ing?

Last Answer : Sodium hydroxide

All the following statements about recognition of a codon on mRNA by an anticodon on tRNA are correct except (A) The recognition of the third base of the codon is not very precise (B) Imprecise recognition of the third base results in wobble (C) Wobble is partly responsible for the degeneracy of the genetic code (D) Wobble results in incorporation of incorrect amino acids in the protein

Description : All the following statements about recognition of a codon on mRNA by an anticodon on tRNA are correct except (A) The recognition of the third base of the codon is not very precise (B) ... degeneracy of the genetic code (D) Wobble results in incorporation of incorrect amino acids in the protein

Last Answer : Answer : D

In the process of activation of amino acids for protein synthesis, the number of high energy phosphate bond equivalent utilised is (A) 0 (B) 1 (C) 2 (D) 4

Description : In the process of activation of amino acids for protein synthesis, the number of high energy phosphate bond equivalent utilised is (A) 0 (B) 1 (C) 2 (D) 4

Last Answer : Answer : C

The enzyme amino acyl tRNA synthetase is involved in (A) Dissociation of discharged tRNA from 80S ribosome (B) Charging of tRNA with specific amino acids (C) Termination of protein synthesis (D) Nucleophilic attack on esterified carboxyl group of peptidyl tRNA

Description : The enzyme amino acyl tRNA synthetase is involved in (A) Dissociation of discharged tRNA from 80S ribosome (B) Charging of tRNA with specific amino acids (C) Termination of protein synthesis (D) Nucleophilic attack on esterified carboxyl group of peptidyl tRNA

Last Answer : Answer : B

A molecule of CO2 is captured by biotin when it acts as coenzyme for carboxylation reaction. The carboxyl group is covalently attached to (A) A nitrogen (N1) of the biotin molecule (B) Sulphur of thiophene ring (C) α-Amino group of lysine (D) α-Amino group of protein

Description : A molecule of CO2 is captured by biotin when it acts as coenzyme for carboxylation reaction. The carboxyl group is covalently attached to (A) A nitrogen (N1) of the biotin molecule (B) Sulphur of thiophene ring (C) α-Amino group of lysine (D) α-Amino group of protein

Last Answer : Answer : A

In biotin-containing enzymes, the biotin is bound to the enzyme by (A) An amide linkage to carboxyl group of glutamine (B) A covalent bond with CO2 (C) An amide linkage to an amino group of lysine (D) An amide linkage to α-carboxyl group of protein

Description : In biotin-containing enzymes, the biotin is bound to the enzyme by (A) An amide linkage to carboxyl group of glutamine (B) A covalent bond with CO2 (C) An amide linkage to an amino group of lysine (D) An amide linkage to α-carboxyl group of protein

Last Answer : Answer : C

With the exception of glycine all amino acids found in protein are (A) Isocitrate dehydrogenase (B) Fumarase (C) Succinate thiokinase (D) ATPase

Description : With the exception of glycine all amino acids found in protein are (A) Isocitrate dehydrogenase (B) Fumarase (C) Succinate thiokinase (D) ATPase

Last Answer : Answer : B

Protein is a polymer of (A) Sugars (B) Phenols (C) Amino acids (D) Carboxylic acids

Description : Protein is a polymer of (A) Sugars (B) Phenols (C) Amino acids (D) Carboxylic acids

Last Answer : Answer : C

Out of 200 different amino acids form in nature the number of amino acids present in protein: (A) 20 (B) 25 (C) 40 (D) 35

Description : Out of 200 different amino acids form in nature the number of amino acids present in protein: (A) 20 (B) 25 (C) 40 (D) 35

Last Answer : Answer : A

Tertiary structure of a protein describes (A) The order of amino acids (B) Location of disulphide bonds (C) Loop regions of proteins (D) The ways of protein folding

Description : Tertiary structure of a protein describes (A) The order of amino acids (B) Location of disulphide bonds (C) Loop regions of proteins (D) The ways of protein folding

Last Answer : Answer : D

Hydrophobic bonds are formed in protein be- tween which amino acids?

Description : Hydrophobic bonds are formed in protein be- tween which amino acids?

Last Answer : Valine, leucine and isoleucine residues. 

The given diagrammatic OH OH HOCH O 2 “X” representation shows one of the categories of small molecular weight organic compounds in the living tissues. Identify the category shown and the one blank component “X” in it. Category Component (a) Cholesterol Guanine (b) Amino acid NH2 (c) Nucleotide Adenine (d) Nucleoside Uracil

Description : The given diagrammatic OH OH HOCH O 2 X representation shows one of the categories of small molecular weight organic compounds in the living tissues. Identify the category shown and the one ... Component (a) Cholesterol Guanine (b) Amino acid NH2 (c) Nucleotide Adenine (d) Nucleoside Uracil

Last Answer : (d) Nucleoside Uracil

Identify the basic amino acid from the following. (a) Tyrosine (b) Glutamic Acid (c) Lysine (d) Valine

Description : Identify the basic amino acid from the following. (a) Tyrosine (b) Glutamic Acid (c) Lysine (d) Valine

Last Answer : (c) Lysine

Fist is a first class protein as it Contains - (1) essential amino acids (2) non-essential amino acids (3) all essential fatty acids (4) no amino acid

Description : Fist is a first class protein as it Contains - (1) essential amino acids (2) non-essential amino acids (3) all essential fatty acids (4) no amino acid

Last Answer : (1) essential amino-acids Explanation: Animal proteins derived from meat and fish are called first class proteins. A first-class protein is one which contains all 8 essential amino acids (which ... the body). They are also called complete proteins. Plant proteins are called second class proteins.

Fist is a first class protein as it contains (1) essential amino-acids (2) non-essential amino acids (3) all essential fatty acids (4) no amino acid

Description : Fist is a first class protein as it contains (1) essential amino-acids (2) non-essential amino acids (3) all essential fatty acids (4) no amino acid

Last Answer : essential amino-acids

Assertion `:` Pancreatic amylase digest protein to amino acid. Reason `:` Pancreatic amylase breaks the peptide bond of protein.

Description : Assertion `:` Pancreatic amylase digest protein to amino acid. Reason `:` Pancreatic amylase breaks the peptide bond of protein.

Last Answer : Assertion `:` Pancreatic amylase digest protein to amino acid. Reason `:` Pancreatic amylase breaks the ... D. If both Assertion & Reason are false.

What type of mutation results in the formation of a protein with one incorrect amino acid?

Description : What type of mutation results in the formation of a protein with one incorrect amino acid?

Last Answer : What is the answer ?

Protein that contains only amino acid

Description : Protein that contains only amino acid

Last Answer : Ans. Simple protein

Which of the following statement(s) is/are true concerning protein/amino acid metabolism in man? a. The major source of amino acids is breakdown of circulating proteins b. The recommended daily allowance for protein may triple in critically ill patients c. Urinary nitrogen losses will approach 0 in the face of protein starvation d. Negative nitrogen balance refers to a decrease in nitrogen taken into the body versus the amount of nitrogen lost

Description : Which of the following statement(s) is/are true concerning protein/amino acid metabolism in man? a. The major source of amino acids is breakdown of circulating proteins b. The recommended daily ... balance refers to a decrease in nitrogen taken into the body versus the amount of nitrogen lost

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