Description : Match the following columns and select the correct option. Column-I Column-II (A) Bt cotton (i) Gene therapy (B) Adenosine (ii) Cellular defence deaminase deficiency (C) RNAi (iii) Detection of HIV infection (D) PCR (iv) Bacillus ... (i) (iv) (c) (ii) (iii) (iv) (i) (d) (i) (ii) (iii) (iv)
Last Answer : (a) (iv) (i) (ii) (iii)
Description : The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 ... - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers
Last Answer : (c) C - extension in the presence of heat stable DNA polymerase
Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.
Last Answer : (d) denaturation, annealing, extension.
Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension
Last Answer : (4) Denaturation, Annealing, Extension
Description : A DNA probe and PCR are being used to identify _____. a. HIV infection b. HPV in PAP smears c. coli in water quality tests d. A-C are correct
Last Answer : d. A-C are correct
Description : What is the false-positive rate for an HIV PCR test and when, after exposure, can it be taken?
Last Answer : Try this site, for a fairly good FAQ: http://www.avert.org/hivtesting.htm
Description : What are the applications of PCR in biotechnology? -Biology
Last Answer : answer:
Description : What are the requirements for PCR (Polymerase Chain Reaction)? -Biology
Description : Steps involved in PCR (Polymerase Chain Reaction) process. -Biology
Last Answer : Step 1 DNA isolation.Step 2 Primer design.Step 3 Enzyme selection.Step 4 Thermal cycling.Step 5 Amplicon analysis.
Description : what are the limitations PCR -Biology
Description : What is reverse transcription PCR? -Biology
Description : Why is Taq polymerase used in PCR? -Biology
Last Answer : Taq DNA Polymerase is highly efficient, so it becomes fully functional as it reaches its optimum temperature. It also has a half-life of more than two hours (at a temperature of 92 °C), a high-amplification capacity, and the ability to add 150 nucleotides per second.
Description : Who invented PCR? -Science
Description : What is the full form of PCR ?
Last Answer : The full form of PCR is: Polymer chain reaction.
Description : After 4 PCR cycles how many DNA molecules are formed from one DNA template molecule ?
Last Answer : After 4 PCR cycles how many DNA molecules are formed from one DNA template molecule ? A. 4 B. 32 C. 16 D. 8
Description : How many molecules of DNA would result from the molecule after five cycles of PCR?
Last Answer : The number of double stranded DNA pieces is doubled in each cycle, so that after n cycles you have 2^n (2 to the n:th power) copies of DNA. For example, after 10 cycles you have 1024 copies, after 20 cycles you have about one million copies, etc.
Description : Can RNA be used in PCR?
Last Answer : Reverse transcription PCR, or RT-PCR, allows the use of RNA as a template. An additional step allows the detection and amplification of RNA. The RNA is reverse transcribed into complementary DNA (cDNA), using reverse transcriptase.
Description : What does polymerase chain reaction (pcr) do?
Last Answer : Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.
Description : Twenty cycles of PCR can amplify DNA: (A) 220 fold (B) 202 fold (C) 20 x 2 fold (D) 20 fold
Last Answer : Answer : A
Description : DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication
Last Answer : Answer : C
Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease
Last Answer : Answer : D
Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)
Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway
Description : Which of the followings could be an immunodiagnostic test? B A. PCR B. ELISA C. Liver function test D. Biopsy
Last Answer : ELISA
Description : Following is the most sensitive test to detect the DNA. C A. ELISA B. Magnetic Resonance Imaging C. PCR D. CT Scanning
Last Answer : PCR
Description : Which of the followings could be an immunodiagnostic test? B A. PCR B. ELISA C. Liver function test D. Biops
Description : Fat emboli are best demonstrated in the lungs by C A. PCR B. Chromatography C. Frozen section examination of tissues stained with Sudan red D. None of these
Last Answer : Frozen section examination of tissues stained with Sudan red
Description : Which one is a true statement regarding DNA polymerase used in PCR? (a) It is used to ligate introduced DNA in recipient cells. (b) It serves as a selectable marker. (c) It is isolated from a virus. (d) It remains active at high temperature.
Last Answer : (d) It remains active at high temperature.
Description : PCR and restriction fragment length polymorphism are the methods for (a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.
Last Answer : (d) genetic fingerprinting.
Description : In vitro clonal propagation in plants is characterized by (a) PCR and RAPD (b) Northern blotting (c) electrophoresis and HPLC (d) microscopy.
Last Answer : (a) PCR and RAPD
Description : An analysis of chromosomal DNA using the Southern hybridization technique does not use (a) electrophoresis (b) blotting (c) autoradiography (d) PCR.
Last Answer : (d) PCR.
Description : Agarose extracted from sea weeds is used in (a) spectrophotometry (b) tissue culture (c) PCR (d) gel electrophoresis
Last Answer : (d) gel electrophoresis.
Description : A new field of _________ has arisen from sequencing, analyzing and comparing microbial genomes. a. PCR b. DNA probes c. microbial genomics d. microarrays
Last Answer : c. microbial genomics
Description : The PCR/sequencing approach to rRNA is most likely to give information about A.the species to which the bacteria are most closely related B.morphology of the bacterium C.the type of energy metabolism the bacterium has D.whether the bacterium is motile
Last Answer : A.the species to which the bacteria are most closely related
Description : 8. The DNA polymerase enzyme used in PCR is obtained from (a) Thermus aquaticus (b) Escherichia coli (c) Agrobacterium tumefaciens (d) Salmonella typhimurium.
Last Answer : (a) Thermus aquaticus
Description : 9. . Which of the following steps are catalysed by Taq polymerase in a PCR reaction? (a) Denaturation of template DNA (b) Annealing of primers to template DNA (c) Extension of primer end on the template DNA (d) All of the above
Last Answer : (c) Extension of primer end on the template DNA
Description : 6. The DNA polymerase enzyme used in PCR is obtained from (a) Thermusaquaticus (b) Escherichia coli (c) Agrobacterium tumefaciens (d) Salmonella typhimurium
Last Answer : (c) Agrobacterium tumefaciens
Description : Which one is a true statement regarding DNA polymerase used in PCR ? (1) It remains active at hight temperature (2) It is used to ligate introduced DNA in recipient cells (3) It serves as a selectable marker (4) It is isolated from a virus
Last Answer : (2) It is used to ligate introduced DNA in recipient cells
Description : Which of the following statement is false (a). Enzymes are differentiated by electrophoresis method (b). While DNA sequencing both forward and reverse primers are used (c). Amplification is done through PCR (d). ABO blood grouping is mainly used for differentiating individuals
Last Answer : Ans: (b). While DNA sequencing both forward and reverse primers are used
Description : Select the correct order of osmolarity changes in fillrate w.r.t. plasma in `:-` Bowmans capsul `rarr` PCT `rarr` Descending loop of henle `rarr `Asce
Last Answer : Select the correct order of osmolarity changes in fillrate w.r.t. plasma in `:-` Bowmans ... `rarr` Isotonic `rarr` Hypotonic `rarr` Hypertonic
Description : Select the correct sequence in the decreasing order of contribution to the forest cover on India: [CPO AC 2003] (a) Dense forest - Open forest - Mangrove forest (b) Open forest - Dense forest - ... (c) Dense forest - Mangrove forest - Open forest (d) Open forest - Mangrove forest - Dense forest
Last Answer : Ans: (b)
Description : Match column I with column II for housefly classification and select the correct option using the codes given below. Column I Column II A. Family (i) Diptera B. Order (ii) Arthropoda C. Class (iii)Muscidae D. Phylum (iv) Insecta (a) A-( ... B-(iii), C-(ii), D-(i) (d) A-(iv), B-(ii), C-(i), D-(iii)
Last Answer : (d) A-(iv), B-(ii), C-(i), D-(iii)
Description : Select the correct order for the different phases of virus execution. i) Propagation phase ii) Dormant phase iii) Execution phase iv) Triggering phase A) i, ii, iii, and iv B) i, iii, ii and iv s D) ii, iii, iv and i
Last Answer : C) ii, i, iv an iii
Description : Select the correct hierarchical order of qualities of an individual (a) Ability-Skills- Aptitude- Competency ; (b) Aptitude-Ability- Skills- Competency (c) Skills- Aptitude- Competency- Ability ; (d) Competency-Ability-Skills- Aptitude
Last Answer : (b) Aptitude-Ability- Skills- Competency
Description : Given below are some codes of arrangements of three items in order of wider scope. Select the correct code. (A) Garments, cloth and shirts (B) Cloth, garments and shirts (C) Shirts, garments and cloth (D) Garments, shirts and cloth
Last Answer : (B) Cloth, garments and shirts
Description : Suppose the inventory manager at Hotpoint intends to lower stockout costs. Which of the following is considered a stockout cost that the manager at Hotpoint might address? A)Price of washing ... to short supply of dishwashers C)Order -processing expenses D)Expenses of backordering small appliances
Last Answer : B)Sales lost due to short supply of dishwashers
Description : Greg is a physical distribution manager. He is currently developing and maintaining assortments of products that are adequate for customer demand. In which stage in the physical distribution ... involved? A)Order processing B)Materials handling C)Inventory management D)Transportation E)Warehousing
Last Answer : C)Inventory management
Description : Which of the following physical distribution functions involves the design and operation of facilities for storing and moving goods? A)Order processing B)Materials handling C)Transportation D)Warehousing E)Inventory management
Last Answer : D)Warehousing