Why is Taq polymerase used in PCR? -Biology

Why is Taq polymerase used in PCR? -Biology
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Taq DNA Polymerase is highly efficient, so it becomes fully functional as it reaches its optimum temperature. It also has a half-life of more than two hours (at a temperature of 92 °C), a high-amplification capacity, and the ability to add 150 nucleotides per second.
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Why is Taq polymerase used in PCR? -Biology

Description : Why is Taq polymerase used in PCR? -Biology

Last Answer : Taq DNA Polymerase is highly efficient, so it becomes fully functional as it reaches its optimum temperature. It also has a half-life of more than two hours (at a temperature of 92 °C), a high-amplification capacity, and the ability to add 150 nucleotides per second.

The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Last Answer : Answer : D

Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

Description : 9. . Which of the following steps are catalysed by Taq polymerase in a PCR reaction? (a) Denaturation of template DNA (b) Annealing of primers to template DNA (c) Extension of primer end on the template DNA (d) All of the above

Last Answer : (c) Extension of primer end on the template DNA

In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Last Answer : Answer : D

The Taq polymerase enzyme is obtained from (a) Bacillus subtilis (b) Pseudomonas putida (c) Thermus aquaticus (d) Thiobacillus ferroxidans.

Description : The Taq polymerase enzyme is obtained from (a) Bacillus subtilis (b) Pseudomonas putida (c) Thermus aquaticus (d) Thiobacillus ferroxidans.

Last Answer : (c) Thermus aquaticus

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Last Answer : (d) ligase.

What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

Description : What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

Last Answer : answer:

Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Description : Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Last Answer : Step 1 DNA isolation.Step 2 Primer design.Step 3 Enzyme selection.Step 4 Thermal cycling.Step 5 Amplicon analysis.

What does polymerase chain reaction (pcr) do?

Description : What does polymerase chain reaction (pcr) do?

Last Answer : Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.

The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for both antigen and antibody detection b. Southern, northern, and western immunoblot techniques are used to detect DNA, RNA, or proteins, respectively c. Polymerase chain reaction (PCR) is a sensitive assay used to detect small amounts of microbial DNA, thus detecting infection at its early stages d. Infectious agents currently detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 5 3 5 3 5 3 5 3 5 5 3 3 5 3 5 3 Region to be amplified . (a) B - denaturation at a temperature of about 98°C separating the two DNA strands (b) A - denaturation at a temperature of about 50°C (c) C - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Description : The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 ... - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Last Answer : (c) C - extension in the presence of heat stable DNA polymerase

Which one is a true statement regarding DNA polymerase used in PCR? (a) It is used to ligate introduced DNA in recipient cells. (b) It serves as a selectable marker. (c) It is isolated from a virus. (d) It remains active at high temperature.

Description : Which one is a true statement regarding DNA polymerase used in PCR? (a) It is used to ligate introduced DNA in recipient cells. (b) It serves as a selectable marker. (c) It is isolated from a virus. (d) It remains active at high temperature.

Last Answer : (d) It remains active at high temperature.

The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

Last Answer : (d) denaturation, annealing, extension.

The DNA polymerase enzyme used in PCR is obtained from

Description : 8. The DNA polymerase enzyme used in PCR is obtained from (a) Thermus aquaticus (b) Escherichia coli (c) Agrobacterium tumefaciens (d) Salmonella typhimurium.

Last Answer : (a) Thermus aquaticus

The DNA polymerase enzyme used in PCR is obtained from

Description : 6. The DNA polymerase enzyme used in PCR is obtained from (a) Thermusaquaticus (b) Escherichia coli (c) Agrobacterium tumefaciens (d) Salmonella typhimurium

Last Answer : (c) Agrobacterium tumefaciens

The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension

Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension

Last Answer : (4) Denaturation, Annealing, Extension

Which one is a true statement regarding DNA polymerase used in PCR ? (1) It remains active at hight temperature (2) It is used to ligate introduced DNA in recipient cells (3) It serves as a selectable marker (4) It is isolated from a virus

Description : Which one is a true statement regarding DNA polymerase used in PCR ? (1) It remains active at hight temperature (2) It is used to ligate introduced DNA in recipient cells (3) It serves as a selectable marker (4) It is isolated from a virus

Last Answer : (2) It is used to ligate introduced DNA in recipient cells

What are the applications of PCR in biotechnology? -Biology

Description : What are the applications of PCR in biotechnology? -Biology

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what are the limitations PCR -Biology

Description : what are the limitations PCR -Biology

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What is reverse transcription PCR? -Biology

Description : What is reverse transcription PCR? -Biology

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What is reverse transcription PCR? -Biology

Description : What is reverse transcription PCR? -Biology

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What is the false-positive rate for an HIV PCR test and when, after exposure, can it be taken?

Description : What is the false-positive rate for an HIV PCR test and when, after exposure, can it be taken?

Last Answer : Try this site, for a fairly good FAQ: http://www.avert.org/hivtesting.htm

Who invented PCR? -Science

Description : Who invented PCR? -Science

Last Answer : answer:

What is the full form of PCR ?

Description : What is the full form of PCR ?

Last Answer : The full form of PCR is: Polymer chain reaction.

Select the correct order of processing of PCR

Description : Select the correct order of processing of PCR

Last Answer : Select the correct order of processing of PCR A. Extension, primer annealing, denaturation ... annealing D. Primer annealing, denaturation, extension

After 4 PCR cycles how many DNA molecules are formed from one DNA template molecule ?

Description : After 4 PCR cycles how many DNA molecules are formed from one DNA template molecule ?

Last Answer : After 4 PCR cycles how many DNA molecules are formed from one DNA template molecule ? A. 4 B. 32 C. 16 D. 8

How many molecules of DNA would result from the molecule after five cycles of PCR?

Description : How many molecules of DNA would result from the molecule after five cycles of PCR?

Last Answer : The number of double stranded DNA pieces is doubled in each cycle, so that after n cycles you have 2^n (2 to the n:th power) copies of DNA. For example, after 10 cycles you have 1024 copies, after 20 cycles you have about one million copies, etc.

Can RNA be used in PCR?

Description : Can RNA be used in PCR?

Last Answer : Reverse transcription PCR, or RT-PCR, allows the use of RNA as a template. An additional step allows the detection and amplification of RNA. The RNA is reverse transcribed into complementary DNA (cDNA), using reverse transcriptase.

Twenty cycles of PCR can amplify DNA: (A) 220 fold (B) 202 fold (C) 20 x 2 fold (D) 20 fold

Description : Twenty cycles of PCR can amplify DNA: (A) 220 fold (B) 202 fold (C) 20 x 2 fold (D) 20 fold

Last Answer : Answer : A

DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication

Description : DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication

Last Answer : Answer : C

Which of the followings could be an immunodiagnostic test? B A. PCR B. ELISA C. Liver function test D. Biopsy

Description : Which of the followings could be an immunodiagnostic test? B A. PCR B. ELISA C. Liver function test D. Biopsy 

Last Answer : ELISA

Following is the most sensitive test to detect the DNA. C A. ELISA B. Magnetic Resonance Imaging C. PCR D. CT Scanning

Description : Following is the most sensitive test to detect the DNA. C A. ELISA B. Magnetic Resonance Imaging C. PCR D. CT Scanning

Last Answer : PCR

Which of the followings could be an immunodiagnostic test? B A. PCR B. ELISA C. Liver function test D. Biops

Description : Which of the followings could be an immunodiagnostic test? B A. PCR B. ELISA C. Liver function test D. Biops

Last Answer : ELISA

Fat emboli are best demonstrated in the lungs by C A. PCR B. Chromatography C. Frozen section examination of tissues stained with Sudan red D. None of these

Description : Fat emboli are best demonstrated in the lungs by C A. PCR B. Chromatography C. Frozen section examination of tissues stained with Sudan red D. None of these

Last Answer : Frozen section examination of tissues stained with Sudan red

Match the following columns and select the correct option. Column-I Column-II (A) Bt cotton (i) Gene therapy (B) Adenosine (ii) Cellular defence deaminase deficiency (C) RNAi (iii) Detection of HIV infection (D) PCR (iv) Bacillus thuringiensis (A) (B) (C) (D) (a) (iv) (i) (ii) (iii) (b) (iii) (ii) (i) (iv) (c) (ii) (iii) (iv) (i) (d) (i) (ii) (iii) (iv)

Description : Match the following columns and select the correct option. Column-I Column-II (A) Bt cotton (i) Gene therapy (B) Adenosine (ii) Cellular defence deaminase deficiency (C) RNAi (iii) Detection of HIV infection (D) PCR (iv) Bacillus ... (i) (iv) (c) (ii) (iii) (iv) (i) (d) (i) (ii) (iii) (iv)

Last Answer : (a) (iv) (i) (ii) (iii)

PCR and restriction fragment length polymorphism are the methods for(a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Description : PCR and restriction fragment length polymorphism are the methods for (a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Last Answer : (d) genetic fingerprinting.

In vitro clonal propagation in plants is characterized by (a) PCR and RAPD (b) Northern blotting (c) electrophoresis and HPLC (d) microscopy.

Description : In vitro clonal propagation in plants is characterized by (a) PCR and RAPD (b) Northern blotting (c) electrophoresis and HPLC (d) microscopy.

Last Answer : (a) PCR and RAPD

An analysis of chromosomal DNA using the Southern hybridization technique does not use (a) electrophoresis (b) blotting (c) autoradiography (d) PCR.

Description : An analysis of chromosomal DNA using the Southern hybridization technique does not use (a) electrophoresis (b) blotting (c) autoradiography (d) PCR.

Last Answer : (d) PCR.

Agarose extracted from sea weeds is used in (a) spectrophotometry (b) tissue culture (c) PCR (d) gel electrophoresis

Description : Agarose extracted from sea weeds is used in (a) spectrophotometry (b) tissue culture (c) PCR (d) gel electrophoresis

Last Answer : (d) gel electrophoresis.

A new field of _________ has arisen from sequencing, analyzing and comparing microbial genomes. a. PCR b. DNA probes c. microbial genomics d. microarrays

Description : A new field of _________ has arisen from sequencing, analyzing and comparing microbial genomes. a. PCR b. DNA probes c. microbial genomics d. microarrays

Last Answer : c. microbial genomics

A DNA probe and PCR are being used to identify _____. a. HIV infection b. HPV in PAP smears c. coli in water quality tests d. A-C are correct

Description : A DNA probe and PCR are being used to identify _____. a. HIV infection b. HPV in PAP smears c. coli in water quality tests d. A-C are correct

Last Answer : d. A-C are correct

The PCR/sequencing approach to rRNA is most likely to give information about A.the species to which the bacteria are most closely related B.morphology of the bacterium C.the type of energy metabolism the bacterium has D.whether the bacterium is motile

Description : The PCR/sequencing approach to rRNA is most likely to give information about A.the species to which the bacteria are most closely related B.morphology of the bacterium C.the type of energy metabolism the bacterium has D.whether the bacterium is motile

Last Answer : A.the species to which the bacteria are most closely related

The PCR/sequencing approach to rRNA is most likely to give information about A.the species to which the bacteria are most closely related B.morphology of the bacterium C.the type of energy metabolism the bacterium has D.whether the bacterium is motile

Description : The PCR/sequencing approach to rRNA is most likely to give information about A.the species to which the bacteria are most closely related B.morphology of the bacterium C.the type of energy metabolism the bacterium has D.whether the bacterium is motile

Last Answer : A.the species to which the bacteria are most closely related

Which of the following statement is false (a). Enzymes are differentiated by electrophoresis method (b). While DNA sequencing both forward and reverse primers are used (c). Amplification is done through PCR (d). ABO blood grouping is mainly used for differentiating individuals

Description : Which of the following statement is false (a). Enzymes are differentiated by electrophoresis method (b). While DNA sequencing both forward and reverse primers are used (c). Amplification is done through PCR (d). ABO blood grouping is mainly used for differentiating individuals

Last Answer : Ans: (b). While DNA sequencing both forward and reverse primers are used 

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Description : What is exonuclease activity of DNA polymerase? -Biology

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What is the role of DNA polymerase in DNA replication? -Biology

Description : What is the role of DNA polymerase in DNA replication? -Biology

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What is the function of DNA polymerase? -Biology

Description : What is the function of DNA polymerase? -Biology

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What is the function of DNA polymerase III? -Biology

Description : What is the function of DNA polymerase III? -Biology

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Function of DNA polymerase during DNA replication. -Biology

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