9. . Which of the following steps are catalysed by Taq polymerase in a
PCR reaction?
(a) Denaturation of template DNA
(b) Annealing of primers to template DNA
(c) Extension of primer end on the template DNA
(d) All of the above

1 Answer

Answer :

(c) Extension of primer end on the template DNA

Related questions

Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Last Answer : Answer : D

Description : Why is Taq polymerase used in PCR? -Biology

Last Answer : Taq DNA Polymerase is highly efficient, so it becomes fully functional as it reaches its optimum temperature. It also has a half-life of more than two hours (at a temperature of 92 °C), a high-amplification capacity, and the ability to add 150 nucleotides per second.

Description : Why is Taq polymerase used in PCR? -Biology

Last Answer : Taq DNA Polymerase is highly efficient, so it becomes fully functional as it reaches its optimum temperature. It also has a half-life of more than two hours (at a temperature of 92 °C), a high-amplification capacity, and the ability to add 150 nucleotides per second.

Description : Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Last Answer : Step 1 DNA isolation.Step 2 Primer design.Step 3 Enzyme selection.Step 4 Thermal cycling.Step 5 Amplicon analysis.

Description : The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 ... - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Last Answer : (c) C - extension in the presence of heat stable DNA polymerase

Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

Last Answer : (d) denaturation, annealing, extension.

Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension

Last Answer : (4) Denaturation, Annealing, Extension

Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Last Answer : Answer : D

Description : The Taq polymerase enzyme is obtained from (a) Bacillus subtilis (b) Pseudomonas putida (c) Thermus aquaticus (d) Thiobacillus ferroxidans.

Last Answer : (c) Thermus aquaticus

Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Last Answer : (d) ligase.

Description : What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

Last Answer : answer:

Description : What does polymerase chain reaction (pcr) do?

Last Answer : Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.

Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase

Last Answer : Answer : C

Description : Which one is a true statement regarding DNA polymerase used in PCR? (a) It is used to ligate introduced DNA in recipient cells. (b) It serves as a selectable marker. (c) It is isolated from a virus. (d) It remains active at high temperature.

Last Answer : (d) It remains active at high temperature.

Description : 8. The DNA polymerase enzyme used in PCR is obtained from (a) Thermus aquaticus (b) Escherichia coli (c) Agrobacterium tumefaciens (d) Salmonella typhimurium.

Last Answer : (a) Thermus aquaticus

Description : 6. The DNA polymerase enzyme used in PCR is obtained from (a) Thermusaquaticus (b) Escherichia coli (c) Agrobacterium tumefaciens (d) Salmonella typhimurium

Last Answer : (c) Agrobacterium tumefaciens

Description : Which one is a true statement regarding DNA polymerase used in PCR ? (1) It remains active at hight temperature (2) It is used to ligate introduced DNA in recipient cells (3) It serves as a selectable marker (4) It is isolated from a virus

Last Answer : (2) It is used to ligate introduced DNA in recipient cells

Description : Replication of nuclear DNA in mammals is catalysed by (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase III

Last Answer : Answer : A

Description : In mammals, synthesis of mRNA is catalysed by (A) RNA polymerase I (B) RNA polymerase II (C) RNA polymerase III(D) RNA polymerase IV

Last Answer : Answer : B

Description : All the following statements about charging of tRNA are correct except (A) It is catalysed by amino acyl tRNA synthetase (B) ATP is converted into ADP and Pi in this reaction (C) The enzyme recognizes the tRNA and the amino acid (D) There is a separate enzyme for each tRNA

Last Answer : Answer : B

Description : The first reaction unique to purine nucleotide synthesis is catalysed by (A) PRPP synthetase (B) PRPP glutamyl amido transferase (C) Phosphoribosyl glycinamide synthetase (D) Formyl transferase

Last Answer : Answer : B

Description : The decarboxylation reaction in HMP shunt is catalysed by (A) Gluconolactone hydrolase (B) 6-Phosphogluconate dehydrogenase (C) 6-Phosphogluconate decarboxylase (D) Transaldolase

Last Answer : Answer : C

Description : An allosteric enzyme is generally inhibited by (A) Initial substrate of the pathway (B) Substrate analogues (C) Product of the reaction catalysed by allosteric enzyme (D) Product of the pathway

Last Answer : Answer : D

Description : Galactose 1-phosphate is converted to uridine diphosphate galactose, the reaction is catalysed by the enzyme: (A) Glactokinase (B) Galactose 1-phosphate uridyl transferase (C) Uridine diphospho galactose 4-epimerase (D) UDP glucose pyrophosphorylase

Last Answer : Answer : B

Description : UDP glucose is converted to UDP glucurronate, a reaction catalysed by UDP glucose dehydrogenase requires (A) NAD+ (B) FAD (C) NADP (D) FMN

Last Answer : Answer : A

Description : Glucose 6-phosphate is converted to glucose 1-phosphate in a reaction catalysed by the enzyme phosphoglucomutase, which is (A) Phosphorylated (B) Dephosphorylated (C) Phosphorylated-dephosphorylated (D) Phosphorylated-dephosphorylatedrephosphorylated

Last Answer : Answer : D

Description : The reaction catalysed by α-ketoglutarate dehydrogenase in the citric acid cycle requires (A) NAD (B) NADP (C) ADP (D) ATP

Last Answer : Answer : A

Description : Riboflavin is a coenzyme in the reaction catalysed by the enzyme (A) Acyl CoA synthetase (B) Acyl CoA dehydrogenase (C) β-Hydroxy acyl CoA (D) Enoyl CoA dehydrogenase

Last Answer : Answer : B

Description : The reaction catalysed by phosphofructokinase (A) Is activated by high concentrations of ATP and citrate (B) Uses fruitose-1-phosphate as substrate (C) Is the rate-limiting reaction of the glycolytic pathway (D) Is inhibited by fructose 2, 6-bisphosphate

Last Answer : C

Description : Conversion of glucose to glucose-6-phosphate, the first irreversible reaction of glycolysis, is catalysed by (a) phosphofructokinase (b) aldolase (c) hexokinase (d) enolase.

Last Answer : (c) hexokinase

Description : The product(s) of reaction catalysed by nitrogenase in root nodules of leguminous plants is/are (a) ammonia alone (b) nitrate alone (c) ammonia and oxygen (d) ammonia and hydrogen.

Last Answer : (d) ammonia and hydrogen.

Description : What is the Thiele modulus of the solid catalysed first order reaction as shown in the bellow figure, if the pore diffusion offers negligible resistance to reaction? (A) < 5 (B) < 0.5 (C) > 1 (D) 5

Last Answer : (B) < 0.5

Description : For a solid catalysed chemical reaction, the effectiveness of solid catalyst depends upon the __________ adsorption. (A) Physical (B) Chemical (C) Both (A) and (B) (D) Neither (A) nor (B)

Last Answer : (B) Chemical

Description : For high conversion in a highly exothermic solid catalysed reaction, use a __________ bed reactor. (A) Fixed (B) Fluidised bed reactor followed by a fixed (C) Fixed bed reactor followed by a fluidised (D) Fluidised

Last Answer : (B) Fluidised bed reactor followed by a fixed

Description : A photochemical reaction is (A) Accompanied with emission of light (B) Catalysed by light (C) Initiated by light (D) All (A), (B) & (C)

Last Answer : (C) Initiated by light

Description : Pick out the correct statement. (A) Reactions with high activation energies are very temperature sensitive (B) Chemical equilibrium is a static state (C) A photochemical reaction is catalysed by ... occurs when the energy of the reacting molecule is less than the activation energy of the reaction

Last Answer : (A) Reactions with high activation energies are very temperature sensitive (B) Chemical equilibrium is a static state

Description : In solid catalysed reactions the diffusional effects are more likely to affect the overall rate of reaction for (A) Fast reactions in catalyst of small pore diameter (B) Fast reaction in catalyst ... reactions in catalyst of small pore diameter (D) Slow reactions in catalyst of large pore diameter

Last Answer : (C) Slow reactions in catalyst of small pore diameter

Description : Pick out the wrong statement. (A) Catalytic activity of enzyme catalysed reactions which is affected by temperature, pH value & chemical agents, is maximum at a temperature of about 45°C ... in enzyme catalysed reactions can be calculated by using the thermodynamic properties of substrates & prod

Last Answer : (C) Enzymes help in increasing the activation energy of the reaction

Description : If pore diffusion is the controlling step in a solid catalysed reaction, the catalyst (A) Porosity is very important (B) Porosity is of less importance (C) Internal surface area is utilised efficiently (D) None of these

Last Answer : (B) Porosity is of less importance

Description : . A reaction which is catalysed by an acid is also catalysed by any substance, which has a tendency to (A) Lose a proton (B) Gain a proton (D) None of these (C) Lose an electron

Last Answer : (A) Lose a proton

Description : Kinetics of a solid catalysed reaction can best be studied in a __________ reactor. (A) Batch (B) Plug-flow (C) Mixed (D) None of these

Last Answer : (C) Mixed

Description : A rise in temperature (A) Normally tends to increase the reaction rate (B) Does not affect a catalysed reaction (C) Does not affect photo-chemical reaction rates (D) All (A), (B) and (C)

Last Answer : (D) All (A), (B) and (C)

Description : A reaction which is catalysed by a base is catalysed by all substances which have a tendency to (A) Lose a proton (B) Gain a proton (C) Gain an electron (D) None of these

Last Answer : (B) Gain a proton

Description : A first order gaseous phase reaction is catalysed by a non-porous solid. The kinetic rate constant and the external mass transfer co-efficients are k and kg respectively. The effective rate constant (ke ff) is given by (A) ke ff = k + kg ( ... + kg )/2 (C) ke ff = (kkg ) 1/2 (D) 1/ke ff = 1/k + 1/kg

Last Answer : (D) 1/ke ff = 1/k + 1/kg

Description : The effectiveness factor for large value of Thiele modulus [L√(K/D1 )] of a solid catalysed first order reaction is equal to (where, L = length of the reactor, cm, D1 = diffusion co-efficient, cm2 /second). (A) L √(K/D1 ) (B) 1/[L√(K/D1 )] (C) 1 (D) ∞

Last Answer : (B) 1/[L√(K/D1 )]

Description : A photochemical reaction is __________ light. (A) Initiated by (B) Accompanied with emission of (C) Catalysed by (D) Used to convert heat energy into

Last Answer : (A) Initiated by

Description : Why is polymerase chain reaction important? -Biology

Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.

Description : Why is polymerase chain reaction important? -Biology

Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.

Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Last Answer : use restriction enzymes is the right answer for apex :)