The figure below shows three steps (A, B, C) of
Polymerase Chain Reaction (PCR). Select the
option giving correct identification together with
what it represents?
A.
B.
C
ds DNA 3
5
5
3
5
3 5
3
5
3
5
3
5
5
3 3
5
3
5 3
Region to be amplified
.
(a) B - denaturation at a temperature of about 98°C
separating the two DNA strands
(b) A - denaturation at a temperature of about 50°C
(c) C - extension in the presence of heat stable DNA
polymerase
(d) A - annealing with two sets of primers
Polymerase Chain Reaction (PCR). Select the
option giving correct identification together with
what it represents?
A.
B.
C
ds DNA 3
5
5
3
5
3 5
3
5
3
5
3
5
5
3 3
5
3
5 3
Region to be amplified
.
(a) B - denaturation at a temperature of about 98°C
separating the two DNA strands
(b) A - denaturation at a temperature of about 50°C
(c) C - extension in the presence of heat stable DNA
polymerase
(d) A - annealing with two sets of primers