Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Steps involved in PCR (Polymerase Chain Reaction) process. -Biology
by

1 Answer

Answer :

Step 1 DNA isolation.Step 2 Primer design.Step 3 Enzyme selection.Step 4 Thermal cycling.Step 5 Amplicon analysis.
by

Related questions

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 5 3 5 3 5 3 5 3 5 5 3 3 5 3 5 3 Region to be amplified . (a) B - denaturation at a temperature of about 98°C separating the two DNA strands (b) A - denaturation at a temperature of about 50°C (c) C - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Description : The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 ... - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Last Answer : (c) C - extension in the presence of heat stable DNA polymerase

The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

Last Answer : (d) denaturation, annealing, extension.

The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension

Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension

Last Answer : (4) Denaturation, Annealing, Extension

What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

Description : What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

Last Answer : answer:

Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

Description : 9. . Which of the following steps are catalysed by Taq polymerase in a PCR reaction? (a) Denaturation of template DNA (b) Annealing of primers to template DNA (c) Extension of primer end on the template DNA (d) All of the above

Last Answer : (c) Extension of primer end on the template DNA

What does polymerase chain reaction (pcr) do?

Description : What does polymerase chain reaction (pcr) do?

Last Answer : Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.

The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Last Answer : Answer : D

The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for both antigen and antibody detection b. Southern, northern, and western immunoblot techniques are used to detect DNA, RNA, or proteins, respectively c. Polymerase chain reaction (PCR) is a sensitive assay used to detect small amounts of microbial DNA, thus detecting infection at its early stages d. Infectious agents currently detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

Why is Taq polymerase used in PCR? -Biology

Description : Why is Taq polymerase used in PCR? -Biology

Last Answer : Taq DNA Polymerase is highly efficient, so it becomes fully functional as it reaches its optimum temperature. It also has a half-life of more than two hours (at a temperature of 92 °C), a high-amplification capacity, and the ability to add 150 nucleotides per second.

Why is Taq polymerase used in PCR? -Biology

Description : Why is Taq polymerase used in PCR? -Biology

Last Answer : Taq DNA Polymerase is highly efficient, so it becomes fully functional as it reaches its optimum temperature. It also has a half-life of more than two hours (at a temperature of 92 °C), a high-amplification capacity, and the ability to add 150 nucleotides per second.

Which one is a true statement regarding DNA polymerase used in PCR? (a) It is used to ligate introduced DNA in recipient cells. (b) It serves as a selectable marker. (c) It is isolated from a virus. (d) It remains active at high temperature.

Description : Which one is a true statement regarding DNA polymerase used in PCR? (a) It is used to ligate introduced DNA in recipient cells. (b) It serves as a selectable marker. (c) It is isolated from a virus. (d) It remains active at high temperature.

Last Answer : (d) It remains active at high temperature.

The DNA polymerase enzyme used in PCR is obtained from

Description : 8. The DNA polymerase enzyme used in PCR is obtained from (a) Thermus aquaticus (b) Escherichia coli (c) Agrobacterium tumefaciens (d) Salmonella typhimurium.

Last Answer : (a) Thermus aquaticus

The DNA polymerase enzyme used in PCR is obtained from

Description : 6. The DNA polymerase enzyme used in PCR is obtained from (a) Thermusaquaticus (b) Escherichia coli (c) Agrobacterium tumefaciens (d) Salmonella typhimurium

Last Answer : (c) Agrobacterium tumefaciens

Which one is a true statement regarding DNA polymerase used in PCR ? (1) It remains active at hight temperature (2) It is used to ligate introduced DNA in recipient cells (3) It serves as a selectable marker (4) It is isolated from a virus

Description : Which one is a true statement regarding DNA polymerase used in PCR ? (1) It remains active at hight temperature (2) It is used to ligate introduced DNA in recipient cells (3) It serves as a selectable marker (4) It is isolated from a virus

Last Answer : (2) It is used to ligate introduced DNA in recipient cells

Why is polymerase chain reaction important? -Biology

Description : Why is polymerase chain reaction important? -Biology

Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.

Why is polymerase chain reaction important? -Biology

Description : Why is polymerase chain reaction important? -Biology

Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.

A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Last Answer : use restriction enzymes is the right answer for apex :)

A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Last Answer : use restriction enzymes is the right answer for apex :)

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Last Answer : Answer : C

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Last Answer : Answer : C

In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Last Answer : Answer : D

The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

Description : The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

Last Answer : Answer : C

Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Last Answer : Answer : A

Most commonly employed test for determining the antibody titers for Newcastle disease infection in B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Description : Most commonly employed test for determining the antibody titers for Newcastle disease infection in B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Last Answer : Hemagglutination inhibition

Most commonly employed test for determining the antibody titers for AI infection is B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Description : Most commonly employed test for determining the antibody titers for AI infection is B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Last Answer : Hemagglutination inhibition

Trichomoniasis can be diagnosed by D A. Whiff test B. Polymerase chain reaction C. Wet mount D. All above

Description : Trichomoniasis can be diagnosed by D A. Whiff test B. Polymerase chain reaction C. Wet mount D. All above

Last Answer : All above

DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Description : DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Last Answer : (a) electrophoresis

Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis

Description : Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis

Last Answer : (d) Zinc finger analysis

The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above

Description : The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above

Last Answer : all above

Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction? A- HIV-1 and HIV-2 viruses B- Hepatitis B virus C- Mycobacterium tuberculosis D- All of these

Description : Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction? A- HIV-1 and HIV-2 viruses B- Hepatitis B virus C- Mycobacterium tuberculosis D- All of these

Last Answer : All of these

An important method used in the rapid identification of a pathogen is a. RNA gene sequencing b. polymerase chain reaction c. molecular taxonomy d. biochemical tests

Description : An important method used in the rapid identification of a pathogen is a. RNA gene sequencing b. polymerase chain reaction c. molecular taxonomy d. biochemical tests

Last Answer : d. biochemical tests

In addition to DNA polymerase and primers what else does a polymerase chain require?

Description : In addition to DNA polymerase and primers what else does a polymerase chain require?

Last Answer : Need answer

The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5’ end of the growing polynucleotide chain (C) DNA polymerases can add nucleotides at both ends of the chain (D) All RNA and DNA polymerases can add nucleotides only at the 3’ end of the growing polynucleotide chain

Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain

Last Answer : Answer : D

What are the main steps that are involved in the hybridization process? -Biology

Description : What are the main steps that are involved in the hybridization process? -Biology

Last Answer : answer:

What are the main steps that are involved in the hybridization process? -Biology

Description : What are the main steps that are involved in the hybridization process? -Biology

Last Answer : answer:

The mammalian DNA polymerase involved in error correction is (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase δ

Description : The mammalian DNA polymerase involved in error correction is (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase δ

Last Answer : Answer : B

The following are true about DNA synthesis: a. it requires DNA polymerase b. reverse transcriptase enzymes are involved c. moves in a 5'---> 3' direction d. the rate of error in DNA synthesis is 1 in 105 base pairs

Description : The following are true about DNA synthesis: a. it requires DNA polymerase b. reverse transcriptase enzymes are involved c. moves in a 5'---> 3' direction d. the rate of error in DNA synthesis is 1 in 105 base pairs

Last Answer : it requires DNA polymerase

10.(a) An ore on treatment with dilute hydrochloric acid produces brisk effervesces. What type of ore is this? What steps will be required to obtain metal from the enriched ore. , (b) Copper coin is kept immersed in silver nitrate solution for some time. What change will take place in coin and colour of the solution ? Write balanced chemical equation of the reaction involved. -Chemistry

Description : 10.(a) An ore on treatment with dilute hydrochloric acid produces brisk effervesces. What type of ore is this? What steps will be required to obtain metal from the enriched ore. , (b) ... in coin and colour of the solution ? Write balanced chemical equation of the reaction involved. -Chemistry

Last Answer : (a) Carbonate ore: (i) Calcination: Carbonate ore is heated in limited supply of air and oxide is obtained, e.g. (b) Copper, being more reactive than silver will displace silver from silver nitrate ... and there will be deposition of silver on copper coin. The colour of solution will turn to blue.

A chain growth polymerisation reaction consists of three different types of reaction namely initiation reaction, propagation reaction & termination reaction. Chain growth polymerisation reaction is not involved in the manufacture of (A) Siloxane elastomers (B) Polyamides (C) Vinyl polymers (D) Urea-formaldehyde resins

Description : A chain growth polymerisation reaction consists of three different types of reaction namely initiation reaction, propagation reaction & termination reaction. Chain growth polymerisation reaction is not involved ... Siloxane elastomers (B) Polyamides (C) Vinyl polymers (D) Urea-formaldehyde resins

Last Answer : (D) Urea-formaldehyde resins

What are the steps involved in the complex process of resource planning? -SST 10th

Description : What are the steps involved in the complex process of resource planning? -SST 10th

Last Answer : Resource planning is a complex process which involves : (i) Identification of resources across the country through surveying, mapping and preparation of inventory of resources through their quantitave ... goals of resource planning right from the First Five Year Plan launched after Independence.

Define process of nuclear fission. Writ the steps involved in generating electricity in a nuclear reactor. -Science

Description : Define process of nuclear fission. Writ the steps involved in generating electricity in a nuclear reactor. -Science

Last Answer : Nuclear Fission: The process in which a heavy nucleus (such as uranium, plutonium or thorium) is broken into two nearly equal fragments when bombarded with low-energy neutrons and a tremendous ... turbines producing electricity.The steam cools back into water, which can then be used over again.

What are the basic processing steps involved in BiCMOS process? Additional masks defining P base region

Description : What are the basic processing steps involved in BiCMOS process? Additional masks defining P base region

Last Answer : _ N Collector area _ Buried Sub collector (SCCD) _ Processing steps in CMOS process

.What are the steps involved in twin-tub process?

Description : .What are the steps involved in twin-tub process?

Last Answer : Tub Formation _ Thin-oxide Construction _ Source & Drain Implantation _ Contact cut definition _ Metallization.

What Are The Steps Involved In The Concreting Process, Explain?

Description : What Are The Steps Involved In The Concreting Process, Explain?

Last Answer : Answer : 1. Batching: The process of measurement of the different materials for the making of concrete is known as batching. batching is usually done in two ways: volume batching and weight batching ... performed. Compaction is generally performed in two ways: by hand or by the use of vibrators.

Which of the following is not one of the major steps involved in the marketing research process? A. Defining the problem and research objectives B. Developing the research plan C. Collecting and tabulating data D. Establishing a marketing information system E. Interpreting and reporting the findings.

Description : Which of the following is not one of the major steps involved in the marketing research process? A. Defining the problem and research objectives B. Developing the research plan C. ... and tabulating data D. Establishing a marketing information system E. Interpreting and reporting the findings.

Last Answer : D. Establishing a marketing information system

List the steps involved in slugging process

Description : List the steps involved in slugging process

Last Answer : i) Sieving ii) Weighing iii) Blending iv) Slugging v) Screening vi) Blending vii) Compression Or. Drug + Excipients → Blending → Slugging(formation of big size tablet) → Screening → Blending → Compression.

State the steps involved in Environmental Impact Assessment process.

Description : State the steps involved in Environmental Impact Assessment process.

Last Answer : EIA process 1. Screening - It is the first key decision of EIA process. The purpose of screening is to determine whether a proposal require an EIA or not. 2. Initial environmental ... Both result in a statement of facts, which certifies that practice is in accordance with standard procedure.

Explain in brief steps involved in process planning.

Description : Explain in brief steps involved in process planning.

Last Answer : Steps involved in process planning: 1. Analysis of Product and its specification: - During analysis, following parameter should be considered, a. Drawing and specification of product. b. Quality of ... and fixing the rates of payment. 11. Prepare the route sheet and operation sheet.

What are the applications of PCR in biotechnology? -Biology

Description : What are the applications of PCR in biotechnology? -Biology

Last Answer : answer:

what are the limitations PCR -Biology

Description : what are the limitations PCR -Biology

Last Answer : answer: