The DNA polymerase enzyme used in PCR is obtained from

8. The DNA polymerase enzyme used in PCR is obtained from
(a) Thermus aquaticus
(b) Escherichia coli
(c) Agrobacterium tumefaciens
(d) Salmonella typhimurium.
by

1 Answer

Answer :

(a) Thermus aquaticus
by

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The DNA polymerase enzyme used in PCR is obtained from

Description : 6. The DNA polymerase enzyme used in PCR is obtained from (a) Thermusaquaticus (b) Escherichia coli (c) Agrobacterium tumefaciens (d) Salmonella typhimurium

Last Answer : (c) Agrobacterium tumefaciens

The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for both antigen and antibody detection b. Southern, northern, and western immunoblot techniques are used to detect DNA, RNA, or proteins, respectively c. Polymerase chain reaction (PCR) is a sensitive assay used to detect small amounts of microbial DNA, thus detecting infection at its early stages d. Infectious agents currently detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

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Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Last Answer : Answer : D

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 5 3 5 3 5 3 5 3 5 5 3 3 5 3 5 3 Region to be amplified . (a) B - denaturation at a temperature of about 98°C separating the two DNA strands (b) A - denaturation at a temperature of about 50°C (c) C - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

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Which one is a true statement regarding DNA polymerase used in PCR? (a) It is used to ligate introduced DNA in recipient cells. (b) It serves as a selectable marker. (c) It is isolated from a virus. (d) It remains active at high temperature.

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Which one is a true statement regarding DNA polymerase used in PCR ? (1) It remains active at hight temperature (2) It is used to ligate introduced DNA in recipient cells (3) It serves as a selectable marker (4) It is isolated from a virus

Description : Which one is a true statement regarding DNA polymerase used in PCR ? (1) It remains active at hight temperature (2) It is used to ligate introduced DNA in recipient cells (3) It serves as a selectable marker (4) It is isolated from a virus

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What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

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Last Answer : answer:

Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Description : Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Last Answer : Step 1 DNA isolation.Step 2 Primer design.Step 3 Enzyme selection.Step 4 Thermal cycling.Step 5 Amplicon analysis.

Why is Taq polymerase used in PCR? -Biology

Description : Why is Taq polymerase used in PCR? -Biology

Last Answer : Taq DNA Polymerase is highly efficient, so it becomes fully functional as it reaches its optimum temperature. It also has a half-life of more than two hours (at a temperature of 92 °C), a high-amplification capacity, and the ability to add 150 nucleotides per second.

Why is Taq polymerase used in PCR? -Biology

Description : Why is Taq polymerase used in PCR? -Biology

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What does polymerase chain reaction (pcr) do?

Description : What does polymerase chain reaction (pcr) do?

Last Answer : Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.

The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

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Last Answer : (d) denaturation, annealing, extension.

Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

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Last Answer : (c) Extension of primer end on the template DNA

The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension

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The Taq polymerase enzyme is obtained from (a) Bacillus subtilis (b) Pseudomonas putida (c) Thermus aquaticus (d) Thiobacillus ferroxidans.

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Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these

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RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III

Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III

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In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase

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Carbonic anhydrase enzyme has maximum turn over number (36 million). Minimum turn over number for an enzyme: (A) DNA polymerase (B) Lysozyme (C) Penicillase (D) Lactase dehydrogenase

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An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix of DNA by an enzyme known as DNA polymerase b. The initial product of DNA transcription is called heterogeneous nuclear RNA which codes directly for proteins c. After processing is complete, the mRNA is exported from the nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

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The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNA polymerase

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What characteristics of DNA polymerase III make it an ideal enzyme for replicating the whole genome?

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Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase

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The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase

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The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

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Last Answer : Answer : C

After 4 PCR cycles how many DNA molecules are formed from one DNA template molecule ?

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How many molecules of DNA would result from the molecule after five cycles of PCR?

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Twenty cycles of PCR can amplify DNA: (A) 220 fold (B) 202 fold (C) 20 x 2 fold (D) 20 fold

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DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication

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PCR and restriction fragment length polymorphism are the methods for(a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

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An analysis of chromosomal DNA using the Southern hybridization technique does not use (a) electrophoresis (b) blotting (c) autoradiography (d) PCR.

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A new field of _________ has arisen from sequencing, analyzing and comparing microbial genomes. a. PCR b. DNA probes c. microbial genomics d. microarrays

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A DNA probe and PCR are being used to identify _____. a. HIV infection b. HPV in PAP smears c. coli in water quality tests d. A-C are correct

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Which of the following statement is false (a). Enzymes are differentiated by electrophoresis method (b). While DNA sequencing both forward and reverse primers are used (c). Amplification is done through PCR (d). ABO blood grouping is mainly used for differentiating individuals

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The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above

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The removal of which enzyme affects the synthesis of hnRNA in eukaryotes a) RNA polymerase II b) RNA primase c) RNA polymerase III d) RNA polymerase I

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Unidirectional tandem gene arrays are significant in ________ a. Increase in recombinant gene product with increasing plasmid number. b. Increase in recombinant gene product without increasing plasmid number. c. Decrease in polymerase enzyme battery with increasing plasmid numbers. d. Decrease in strong promotor with increase in plasmid number

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What is the difference between DNA and RNA polymerase?

Description : What is the difference between DNA and RNA polymerase?

Last Answer : DNA polymerase is responsible for synthesizing new strands of DNA during DNA replication, while RNA polymerase is responsible for synthesizing mRNA during transcription.

What is exonuclease activity of DNA polymerase? -Biology

Description : What is exonuclease activity of DNA polymerase? -Biology

Last Answer : answer:

What is the role of DNA polymerase in DNA replication? -Biology

Description : What is the role of DNA polymerase in DNA replication? -Biology

Last Answer : answer:

What is the function of DNA polymerase? -Biology

Description : What is the function of DNA polymerase? -Biology

Last Answer : answer:

What is the function of DNA polymerase III? -Biology

Description : What is the function of DNA polymerase III? -Biology

Last Answer : answer:

Function of DNA polymerase during DNA replication. -Biology

Description : Function of DNA polymerase during DNA replication. -Biology

Last Answer : answer:

When is DNA polymerase used? -Biology

Description : When is DNA polymerase used? -Biology

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When is DNA polymerase used? -Biology

Description : When is DNA polymerase used? -Biology

Last Answer : answer:

How does DNA polymerase prevent mutation?

Description : How does DNA polymerase prevent mutation?

Last Answer : It checks DNA for errors during replication.

DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the

Description : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the

Last Answer : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the ... Antistrand C. Template strand D. Coding strand

When does. DNA polymerase check for mutations?

Description : When does. DNA polymerase check for mutations?

Last Answer : During DNA replication