Description : DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III
Last Answer : Answer : B
Description : One of the first enzymes synthesized by many bacteriophage is __________ , an RNA dependent RNA polymerase A.RNA transcriptase B.RNA polymerase C.RNA ligase D.RNA replicase
Last Answer : D.RNA replicase
Description : Another name for reverse transcriptase is (A) DNA dependent DNA polymerase (B) DNA dependent RNA polymerase (C) RNA dependent DNA polymerase (D) RNA dependent RNA polymerase
Last Answer : Answer : C
Description : All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses
Last Answer : Answer : D
Description : Which one of the following makes use of RNA template to synthesise DNA? (a) DNA polymerase (b) RNA polymerase (c) Reverse transcriptase (d) DNA dependant RNA polymerase
Last Answer : c) Reverse transcriptase
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes
Description : Reverse transcriptase activity is present in the eukaryotic: (A) DNA polymerase α (B) DNA polymerase γ (C) Telomerase (D) DNA polymerase II
Description : Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Last Answer : Answer : A
Description : Deoxyribonucleotides are added to RNA primer by (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III
Description : The only correct statement about oncoviruses is (A) All the oncoviruses are RNA viruses (B) Reverse transcriptase is present in all oncoviruses (C) Viral oncogenes are identical to human protooncogens (D) Both DNA and RNA viruses can be oncoviruses
Description : Reverse transcriptase catalyses (A) Synthesis of RNA (B) Breakdown of RNA (C) Synthesis of DNA (D) Breakdown of DNA
Description : Reverse transcriptase is capable of synthesising (A) RNA → DNA (B) DNA → RNA (C) RNA → RNA (D) DNA → DNA
Description : The following are true about DNA synthesis: a. it requires DNA polymerase b. reverse transcriptase enzymes are involved c. moves in a 5'---> 3' direction d. the rate of error in DNA synthesis is 1 in 105 base pairs
Last Answer : it requires DNA polymerase
Description : In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase
Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase
Last Answer : d. RNA polymerase
Description : In which organism did the reverse transcriptase enzyme which performs DNA synthesis from RNA templates first appear?
Last Answer : Need answer
Description : RNA interference involves (a) synthesis of cDNA and RNA using reverse transcriptase (b) silencing of specific mRNA due to complementary RNA (c) interference of RNA in synthesis of DNA (d) synthesis of mRNA from DNA
Last Answer : (b) silencing of specific mRNA due to complementary RNA
Description : Retroviruses are so-named because their reverse transcriptase _____. a. reverses the flow of genetic information by stimulating information to proceed from RNA to DNA. b. reverses the normal ... the action of drugs on viruses and allows them to continue multiplying in living cells.
Last Answer : a. reverses the flow of genetic information by stimulating information to proceed from RNA to DNA.
Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin
Description : The antibiotic which inhibits DNA dependent RNA polymerase is (A) Mitomycin C (B) Actinomycin d (C) Streptomycin (D) Puromycin
Description : α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase
Description : A consensus sequence on DNA, called TATA box, is the site for attachment of (A) RNA-dependent DNA polymerase (B) DNA-dependent RNA polymerase (C) DNA-dependent DNA polymerase (D) DNA topoisomerase II
Description : The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Description : The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Description : DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these
Description : DNA-dependent RNA polymerase is a (A) Monomer (B) Dimer (C) Trimer (D) Tetramer
Description : The region of DNA known as TATA BOX is the site for binding of (A) DNA polymerase (B) DNA topoisomerase (C) DNA dependent RNA polymerase (D) Polynucleotide phosphorylase
Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sites of genes is inhibited by the antibiotic: (A) Puromycin (B) Rifamycin (C) Terramycin (D) Streptomycin
Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase
Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain
Description : In mammals, synthesis of mRNA is catalysed by (A) RNA polymerase I (B) RNA polymerase II (C) RNA polymerase III(D) RNA polymerase IV
Description : Mammalian RNA polymerase III synthesises (A) rRNA (B) mRNA (C) tRNA (D) hnRNA
Description : The virus directed reverse transcriptase enzyme is inhibited by: A. Amantadine B. Zidovudine C. Vidarabine D. Acyclovir
Last Answer : B. Zidovudine
Description : The antiviral action of amantadine is exerted through: A. Interaction with the viral M2 protein B. Interaction with a virus directed thymidine kinase C. Inhibition of a viral protease enzyme D. Inhibition of viral RNA mediated DNA synthesi
Last Answer : A. Interaction with the viral M2 protein
Description : Ciprofloxacin inhibits (A) DNA topisomerase II (B) DNA polymerase I (C) DNA polymerase III (D) DNA gyrase
Description : Replication of nuclear DNA in mammals is catalysed by (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase III
Description : DNA polymerase III holoenzyme possesses (A) Polymerase activity (B) 3’→5’ Exonuclease activity (C) 5’→3’ Exonuclease and polymerase activities (D) 3’→5’ Exonuclease and polymerase activities
Description : Negative supercoils are introduced in DNA by (A) Helicase (B) DNA ligase (C) DNA gyrase (D) DNA polymerase III holoenzyme
Description : What is the difference between DNA and RNA polymerase?
Last Answer : DNA polymerase is responsible for synthesizing new strands of DNA during DNA replication, while RNA polymerase is responsible for synthesizing mRNA during transcription.
Description : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the
Last Answer : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the ... Antistrand C. Template strand D. Coding strand
Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)
Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway
Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand
Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete
Description : The mechanism of antibacterial action of tetracycline involves (a) Binding to a component of the 50S ribosomal subunit (b) Inhibition of translocase activity (c) Blockade of binding of ... (d) Selective inhibition of ribosomal peptidyl transferases (e) Inhibition of DNA-dependent RNA polymerase
Last Answer : Ans: C
Description : What is antisense technology? (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene (b) RNA polymerase producing DNA (c) A cell displaying a foreign antigen used for synthesis of antigens (d) Production of somaclonal variants in tissue cultures
Last Answer : (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
Description : During transcription, the DNA site at which RNA polymerase binds is called (a) promoter (b) regulator (c) receptor (d) enhancer.
Last Answer : d) enhancer
Description : During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box
Last Answer : (c) GGTT box
Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand
Last Answer : (a) template strand
Description : Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase
Last Answer : b) DNA helicase