RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III

RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III
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Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these

Description : Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these

Last Answer : Answer : A

The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Last Answer : Answer : D

The removal of which enzyme affects the synthesis of hnRNA in eukaryotes a) RNA polymerase II b) RNA primase c) RNA polymerase III d) RNA polymerase I

Description : The removal of which enzyme affects the synthesis of hnRNA in eukaryotes a) RNA polymerase II b) RNA primase c) RNA polymerase III d) RNA polymerase I

Last Answer : a) RNA polymerase II

Deoxyribonucleotides are added to RNA primer by (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these

Description : Deoxyribonucleotides are added to RNA primer by (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these

Last Answer : Answer : C

The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNA polymerase

Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNA polymerase

Last Answer : (3) Helicase Explanation: A type of helicase enzyme called DNA helicase catalyzes the unwinding of the DNA helix at the time of replication. It uses the energy released through hydrolysis of ATP ( ... The enzyme is needed to separate the two strands of DNA also during transcription and DNA repair.

In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core enzymes of RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation

Description : In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core ... RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation

Last Answer : Answer : C

In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Last Answer : Answer : D

Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ

Description : Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ

Last Answer : Answer : B

Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteose

Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteose

Last Answer : a) RNA polymerase

Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteoses

Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteoses

Last Answer : (c) RNA ligase

The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase

Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase

Last Answer : d. RNA polymerase

Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease

Description : Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease

Last Answer : a) Ligase

RNA directed DNA polymerase is (A) Replicase (B) Transcriptase (C) Reversetranscriptase (D) Polymerase–III

Description : RNA directed DNA polymerase is (A) Replicase (B) Transcriptase (C) Reversetranscriptase (D) Polymerase–III

Last Answer : Answer : C

DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III

Description : DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III

Last Answer : Answer : B

The degradation of RNA by pancreatic ribonuclease produces (A) Nucleoside 2-Phosphates (B) Nucleoside 5′-phosphates (C) Oligonucleosides (D) Nucleoside 3′-phosphate and oligonucleotide

Description : The degradation of RNA by pancreatic ribonuclease produces (A) Nucleoside 2-Phosphates (B) Nucleoside 5′-phosphates (C) Oligonucleosides (D) Nucleoside 3′-phosphate and oligonucleotide

Last Answer : Answer : D

The mechanism of synthesis of DNA and RNA are similar to all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is (D) They require a primer

Description : The mechanism of synthesis of DNA and RNA are similar to all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is (D) They require a primer

Last Answer : Answer : D

The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Last Answer : Answer : D

The mechanism of synthesis of DNA and RNA are similar in all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is 5’ → 3’ (D) They require a primer

Description : The mechanism of synthesis of DNA and RNA are similar in all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is 5’ → 3’ (D) They require a primer

Last Answer : Answer : A

RNA synthesis requires (A) RNA primer (B) RNA template (C) DNA template (D) DNA primer

Description : RNA synthesis requires (A) RNA primer (B) RNA template (C) DNA template (D) DNA primer

Last Answer : Answer : C

The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for both antigen and antibody detection b. Southern, northern, and western immunoblot techniques are used to detect DNA, RNA, or proteins, respectively c. Polymerase chain reaction (PCR) is a sensitive assay used to detect small amounts of microbial DNA, thus detecting infection at its early stages d. Infectious agents currently detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix of DNA by an enzyme known as DNA polymerase b. The initial product of DNA transcription is called heterogeneous nuclear RNA which codes directly for proteins c. After processing is complete, the mRNA is exported from the nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete

Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase

Description : Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase

Last Answer : b) DNA helicase

The first enzyme isolated, purified and crystallied from Jack bean (Canavalia) by summer in 1926 was (A) Urease (B) Insulin (C) Ribonuclease (D) Zymase

Description : The first enzyme isolated, purified and crystallied from Jack bean (Canavalia) by summer in 1926 was (A) Urease (B) Insulin (C) Ribonuclease (D) Zymase

Last Answer : Answer : A

What is the role of the enzyme DNA primase?

Description : What is the role of the enzyme DNA primase?

Last Answer : Primase is an enzyme that synthesizes short RNA sequences called primers which serve as a starting point for DNA synthesis. Since primase produces RNA molecules, the enzyme is a type of RNA ... strand of nucleotides. Therefore, primase serves to prime and lay a foundation for DNA synthesis.

Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Last Answer : a) Topoisomease II

The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNApolymerase

Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNApolymerase

Last Answer : C option is the answer

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Last Answer : Answer : C

The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin

Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin

Last Answer : Answer : B

Another name for reverse transcriptase is (A) DNA dependent DNA polymerase (B) DNA dependent RNA polymerase (C) RNA dependent DNA polymerase (D) RNA dependent RNA polymerase

Description : Another name for reverse transcriptase is (A) DNA dependent DNA polymerase (B) DNA dependent RNA polymerase (C) RNA dependent DNA polymerase (D) RNA dependent RNA polymerase

Last Answer : Answer : C

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Last Answer : Answer : C

The antibiotic which inhibits DNA dependent RNA polymerase is (A) Mitomycin C (B) Actinomycin d (C) Streptomycin (D) Puromycin

Description : The antibiotic which inhibits DNA dependent RNA polymerase is (A) Mitomycin C (B) Actinomycin d (C) Streptomycin (D) Puromycin

Last Answer : Answer : B

α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase

Description : α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase

Last Answer : Answer : C

A consensus sequence on DNA, called TATA box, is the site for attachment of (A) RNA-dependent DNA polymerase (B) DNA-dependent RNA polymerase (C) DNA-dependent DNA polymerase (D) DNA topoisomerase II

Description : A consensus sequence on DNA, called TATA box, is the site for attachment of (A) RNA-dependent DNA polymerase (B) DNA-dependent RNA polymerase (C) DNA-dependent DNA polymerase (D) DNA topoisomerase II

Last Answer : Answer : B

The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Description : The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Last Answer : Answer : D

The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Description : The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Last Answer : Answer : C

DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these

Description : DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these

Last Answer : Answer : C

DNA-dependent RNA polymerase is a (A) Monomer (B) Dimer (C) Trimer (D) Tetramer

Description : DNA-dependent RNA polymerase is a (A) Monomer (B) Dimer (C) Trimer (D) Tetramer

Last Answer : Answer : D

All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses

Description : All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses

Last Answer : Answer : D

The region of DNA known as TATA BOX is the site for binding of (A) DNA polymerase (B) DNA topoisomerase (C) DNA dependent RNA polymerase (D) Polynucleotide phosphorylase

Description : The region of DNA known as TATA BOX is the site for binding of (A) DNA polymerase (B) DNA topoisomerase (C) DNA dependent RNA polymerase (D) Polynucleotide phosphorylase

Last Answer : Answer : C

The binding of prokaryotic DNA dependent RNA polymerase to promoter sites of genes is inhibited by the antibiotic: (A) Puromycin (B) Rifamycin (C) Terramycin (D) Streptomycin

Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sites of genes is inhibited by the antibiotic: (A) Puromycin (B) Rifamycin (C) Terramycin (D) Streptomycin

Last Answer : Answer : B

The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase

Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase

Last Answer : Answer : C

The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5’ end of the growing polynucleotide chain (C) DNA polymerases can add nucleotides at both ends of the chain (D) All RNA and DNA polymerases can add nucleotides only at the 3’ end of the growing polynucleotide chain

Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain

Last Answer : Answer : D

What characteristics of DNA polymerase III make it an ideal enzyme for replicating the whole genome?

Description : What characteristics of DNA polymerase III make it an ideal enzyme for replicating the whole genome?

Last Answer : The key features of DNA polymerase III are its catalytic potency, its fidelity, and its processivity. It is able to catalyze the addition of ~103 bases per second, compared to only about 10 per ... to dissociate from the template strand after synthesis of short (tens of bases) stretches of DNA.  

In mammals, synthesis of mRNA is catalysed by (A) RNA polymerase I (B) RNA polymerase II (C) RNA polymerase III(D) RNA polymerase IV

Description : In mammals, synthesis of mRNA is catalysed by (A) RNA polymerase I (B) RNA polymerase II (C) RNA polymerase III(D) RNA polymerase IV

Last Answer : Answer : B

Mammalian RNA polymerase III synthesises (A) rRNA (B) mRNA (C) tRNA (D) hnRNA

Description : Mammalian RNA polymerase III synthesises (A) rRNA (B) mRNA (C) tRNA (D) hnRNA

Last Answer : Answer : C

In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase

Description : In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase

Last Answer : Answer : B

Carbonic anhydrase enzyme has maximum turn over number (36 million). Minimum turn over number for an enzyme: (A) DNA polymerase (B) Lysozyme (C) Penicillase (D) Lactase dehydrogenase

Description : Carbonic anhydrase enzyme has maximum turn over number (36 million). Minimum turn over number for an enzyme: (A) DNA polymerase (B) Lysozyme (C) Penicillase (D) Lactase dehydrogenase

Last Answer : Answer : B

After formation of replication fork (A) Both the new strands are synthesized discontinuously (B) One strand is synthesized continuously and the other discontinuously (C) Both the new strands are synthesized continuously (D) RNA primer is required only for the synthesis of one new strand

Description : After formation of replication fork (A) Both the new strands are synthesized discontinuously (B) One strand is synthesized continuously and the other discontinuously (C) Both the new strands are synthesized continuously (D) RNA primer is required only for the synthesis of one new strand

Last Answer : Answer : B

Formation of RNA primer: (A) Precedes replication (B) Follows replication (C) Precedes transcription (D) Follows transcription

Description : Formation of RNA primer: (A) Precedes replication (B) Follows replication (C) Precedes transcription (D) Follows transcription

Last Answer : Answer : A

Ciprofloxacin inhibits (A) DNA topisomerase II (B) DNA polymerase I (C) DNA polymerase III (D) DNA gyrase

Description : Ciprofloxacin inhibits (A) DNA topisomerase II (B) DNA polymerase I (C) DNA polymerase III (D) DNA gyrase

Last Answer : Answer : D