Description : The region of DNA known as TATA BOX is the site for binding of (A) DNA polymerase (B) DNA topoisomerase (C) DNA dependent RNA polymerase (D) Polynucleotide phosphorylase
Last Answer : Answer : C
Description : All of the following statements about bacterial promoters are true except (A) They are smaller than eukaryotic promoters (B) They have two consensus sequences upstream from the transcription star site (C) ... is the site for attachment of RNA polymerase (D) TATA box has a high melting temperature
Last Answer : Answer : D
Description : During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box
Last Answer : (c) GGTT box
Last Answer : (b) TATA box
Description : All of the following statements about eukaryotic promoters are true except (A) They may be located upstream or down stream from the structural gene (B) They have two consensus sequences ( ... (D) Mutations in promoter region can decrease the efficiency of transcription of the structural gene
Last Answer : Answer : A
Description : The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Description : The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Description : DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II
Last Answer : Answer : B
Description : α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase
Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin
Description : Another name for reverse transcriptase is (A) DNA dependent DNA polymerase (B) DNA dependent RNA polymerase (C) RNA dependent DNA polymerase (D) RNA dependent RNA polymerase
Description : The antibiotic which inhibits DNA dependent RNA polymerase is (A) Mitomycin C (B) Actinomycin d (C) Streptomycin (D) Puromycin
Description : DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these
Description : DNA-dependent RNA polymerase is a (A) Monomer (B) Dimer (C) Trimer (D) Tetramer
Description : All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses
Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sites of genes is inhibited by the antibiotic: (A) Puromycin (B) Rifamycin (C) Terramycin (D) Streptomycin
Description : From a DNA-RNA hybrid, DNA can be obtained by addition of (A) DNA B protein and ATP (B) Helicase and ATP (C) DNA topoisomerase I (D) Alkali
Description : Vinca alkaloids exert antitumor activity by: A. Activating topoisomerase II to cause breaks in DNA strands B. Crosslinking DNA strands C. Inhibiting DNA mediated RNA synthesis D. Inhibiting polymerization of tubulin to form intracellular microtubules
Last Answer : D. Inhibiting polymerization of tubulin to form intracellular microtubules
Description : In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core ... RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation
Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNA polymerase
Last Answer : (3) Helicase Explanation: A type of helicase enzyme called DNA helicase catalyzes the unwinding of the DNA helix at the time of replication. It uses the energy released through hydrolysis of ATP ( ... The enzyme is needed to separate the two strands of DNA also during transcription and DNA repair.
Description : During DNA replication, the strands separate by (a) DNA polymerase (b) topoisomerase (c) unwindase/helicase (d) gyrase.
Last Answer : b) topoisomerase
Description : There are special proteins that help to open up DNA double helix in front of the replication fork. These proteins are (a) DNA ligase (b) DNA topoisomerase I (c) DNA gyrase (d) DNA polymerase I.
Last Answer : (c) DNA gyrase
Description : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the
Last Answer : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the ... Antistrand C. Template strand D. Coding strand
Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand
Last Answer : (a) template strand
Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand.
Description : The mechanism of antibacterial action of tetracycline involves (a) Binding to a component of the 50S ribosomal subunit (b) Inhibition of translocase activity (c) Blockade of binding of ... (d) Selective inhibition of ribosomal peptidyl transferases (e) Inhibition of DNA-dependent RNA polymerase
Last Answer : Ans: C
Description : Which of the following statements is not true for retroviruses? (a) DNA is not present at any stage in the life cycle of retroviruses. (b) Retroviruses carry gene for RNA-dependent DNA ... mature retroviruses is RNA. (d) Retroviruses are causative agents for certain kinds of cancer in man.
Last Answer : (a) DNA is not present at any stage in the life cycle of retroviruses
Description : Rifampin kills tubercle bacilli by: A. Binding to mycobacterial DNA dependent RNA polymerase B. Inhibiting mycobacterial DNA synthesis C. Inhibiting synthesis of mycolic acids in mycobacteria D. Damaging mycobacterial mitochondria
Last Answer : A. Binding to mycobacterial DNA dependent RNA polymerase
Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase
Description : Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Description : Deoxyribonucleotides are added to RNA primer by (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Description : What is antisense technology? (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene (b) RNA polymerase producing DNA (c) A cell displaying a foreign antigen used for synthesis of antigens (d) Production of somaclonal variants in tissue cultures
Last Answer : (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
Description : Eukaryotic promoters contain (A) TATA box 25bp upstream of transcription start site (B) CAAT box 70-80 bp upstream of transcription start site (C) Both (A) and (B) (D) None of these
Description : During transcription, the DNA site at which RNA polymerase binds is called (a) promoter (b) regulator (c) receptor (d) enhancer.
Last Answer : d) enhancer
Last Answer : (a) promoter
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes
Description : RNA directed DNA polymerase is (A) Replicase (B) Transcriptase (C) Reversetranscriptase (D) Polymerase–III
Description : DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III
Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III
Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain
Description : One of the first enzymes synthesized by many bacteriophage is __________ , an RNA dependent RNA polymerase A.RNA transcriptase B.RNA polymerase C.RNA ligase D.RNA replicase
Last Answer : D.RNA replicase
Description : Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Description : RNA polymerase holoenzyme binds to lac operon at the following site: (A) i gene (B) z gene (C) Operator locus (D) Promoter region
Description : DNA topoisomerase I of E. coli catalyses (A) Relaxation of negatively supercoiled DNA (B) Relaxation of positively supercoiled DNA (C) Conversion of negatively supercoiled DNA into positively supercoiled DNA (D) Conversion of double helix into supercoiled DNA
Description : Indicate the enzyme(s) inhibited by fluoroquinolones: A. Both 'A' and 'C' B. Topoisomerase II C. Topoisomerase IV D. DNA gyrase
Last Answer : D. DNA gyrase
Description : In mammals, synthesis of mRNA is catalysed by (A) RNA polymerase I (B) RNA polymerase II (C) RNA polymerase III(D) RNA polymerase IV
Description : Name the steering gear box used in following: (i) Maruti ‘Alto’ (ii) Tata ‘Sumo’ (iii) Ashok Leyland Truck (iv) Road Roller
Last Answer : (i) Maruti ‘Alto’=========Rack and Pinion Type Gear Box. (ii) Tata ‘Sumo’==========Recireulating Ball Type Gear Box. (iii) Ashok Leyland Truck === Worm and Nut Type Gear Box (iv) Road Roller=========== Worm and Roller Type Steering Gear Box
Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand
Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete
Description : What is the difference between DNA and RNA polymerase?
Last Answer : DNA polymerase is responsible for synthesizing new strands of DNA during DNA replication, while RNA polymerase is responsible for synthesizing mRNA during transcription.