The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5’ end of the growing polynucleotide chain (C) DNA polymerases can add nucleotides at both ends of the chain (D) All RNA and DNA polymerases can add nucleotides only at the 3’ end of the growing polynucleotide chain

The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5’ end of the growing polynucleotide chain (C) DNA polymerases can add nucleotides at both ends of the chain (D) All RNA and DNA polymerases can add nucleotides only at the 3’ end of the growing polynucleotide chain
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In addition to DNA polymerase and primers what else does a polymerase chain require?

Description : In addition to DNA polymerase and primers what else does a polymerase chain require?

Last Answer : Need answer

In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Last Answer : Answer : D

The region of DNA known as TATA BOX is the site for binding of (A) DNA polymerase (B) DNA topoisomerase (C) DNA dependent RNA polymerase (D) Polynucleotide phosphorylase

Description : The region of DNA known as TATA BOX is the site for binding of (A) DNA polymerase (B) DNA topoisomerase (C) DNA dependent RNA polymerase (D) Polynucleotide phosphorylase

Last Answer : Answer : C

The 3′ - 5′ phosphodiester linkages inside a polynucleotide chain serve to join (a) one DNA strand with the other DNA strand (b) one nucleoside with another nucleoside (c) one nucleotide with another nucleotide (d) one nitrogenous base with pentose sugar.

Description : The 3′ - 5′ phosphodiester linkages inside a polynucleotide chain serve to join (a) one DNA strand with the other DNA strand (b) one nucleoside with another nucleoside (c) one nucleotide with another nucleotide (d) one nitrogenous base with pentose sugar.

Last Answer : (c) one nucleotide with another nucleotide

The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for both antigen and antibody detection b. Southern, northern, and western immunoblot techniques are used to detect DNA, RNA, or proteins, respectively c. Polymerase chain reaction (PCR) is a sensitive assay used to detect small amounts of microbial DNA, thus detecting infection at its early stages d. Infectious agents currently detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 5 3 5 3 5 3 5 3 5 5 3 3 5 3 5 3 Region to be amplified . (a) B - denaturation at a temperature of about 98°C separating the two DNA strands (b) A - denaturation at a temperature of about 50°C (c) C - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Description : The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 ... - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Last Answer : (c) C - extension in the presence of heat stable DNA polymerase

The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers

Description : The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers

Last Answer : Answer : B

An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix of DNA by an enzyme known as DNA polymerase b. The initial product of DNA transcription is called heterogeneous nuclear RNA which codes directly for proteins c. After processing is complete, the mRNA is exported from the nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete

Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Last Answer : (a) make cuts at specific positions within the DNA molecule

In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core enzymes of RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation

Description : In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core ... RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation

Last Answer : Answer : C

Which of the following nucleoside diphosphates is used most often in carbohydrate anabolism? A.Uridine diphosphate B.Adenosine diphosphate C.Guanine diphosphate D.Thymine diphosphate

Description : Which of the following nucleoside diphosphates is used most often in carbohydrate anabolism? A.Uridine diphosphate B.Adenosine diphosphate C.Guanine diphosphate D.Thymine diphosphate

Last Answer : A.Uridine diphosphate

Which of the following statement(s) is/are true concerning translation of the mRNA message to protein synthesis? a. An adaptor molecule, tRNA, recognizes specific nucleic acid bases and unites them with specific amino acids b. Covalent attachment of tRNA to amino acids is energy dependent c. The formation of a peptide bond between the growing peptide chain and the free amino acid occurs in the free cytoplasm d. Complete protein synthesis takes hours

Description : Which of the following statement(s) is/are true concerning translation of the mRNA message to protein synthesis? a. An adaptor molecule, tRNA, recognizes specific nucleic acid bases and unites them ... and the free amino acid occurs in the free cytoplasm d. Complete protein synthesis takes hours

Last Answer : Answer: a, b The synthesis of protein involves conversion from a four-letter nucleotide language to one of 20 chemically distinct amino acids. This process is referred to as ... translation and be moving down the mRNA molecules simultaneously, thus increasing the rate of protein synthesis

The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase

Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase

Last Answer : d. RNA polymerase

DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication

Description : DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication

Last Answer : Answer : C

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Last Answer : Answer : C

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Last Answer : Answer : C

ATP is called a. A nucleoside b. Physiological currency c. An amino acid d. Polynucleotide

Description : ATP is called a. A nucleoside b. Physiological currency c. An amino acid d. Polynucleotide

Last Answer : b. Physiological currency

A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Last Answer : b. restriction endonuclease

Consider the following statements: (1) Copper is present in cytochrome oxidane (2) Pantothenic acid is precursor of co-enzyme-A (3) Thiamine prohosphate is the prosthetic group in decarboxylases (4) Zinc is present in RNA and DNA polymerases

Description : Consider the following statements: (1) Copper is present in cytochrome oxidane (2) Pantothenic acid is precursor of co-enzyme-A (3) Thiamine prohosphate is the prosthetic group in decarboxylases (4) Zinc is present in RNA and DNA polymerases

Last Answer : Ans. ((b))

RNA is composed of strands of nucleotides that are read as a 3 nucleotide codon. These are distinguished by tRNAs that match the codons on one end and carry individual building blocks of a protein chain. What are these building blocks of protein that tRNAs bind?

Description : RNA is composed of strands of nucleotides that are read as a 3 nucleotide codon. These are distinguished by tRNAs that match the codons on one end and carry individual building blocks of a protein chain. What are these building blocks of protein that tRNAs bind?

Last Answer : Amino Acids

If in a nucleic acid there are more than 8000 nucleotides it is most likely (A) RNA (B) DNA (C) Both (A) and (B) (D) None of these

Description : If in a nucleic acid there are more than 8000 nucleotides it is most likely (A) RNA (B) DNA (C) Both (A) and (B) (D) None of these

Last Answer : Answer : B

Polynucleotide chain of DNA contains (a) a nitrogenous base, deoxyribose sugar and phosphate group (b) a nitrogenous base, ribose sugar and phosphate group (c) deoxyribose sugar ribose sugar and phosphate group (d) a nitrogenous base and phosphate group only

Description : Polynucleotide chain of DNA contains (a) a nitrogenous base, deoxyribose sugar and phosphate group (b) a nitrogenous base, ribose sugar and phosphate group (c) deoxyribose sugar ribose sugar and phosphate group (d) a nitrogenous base and phosphate group only

Last Answer : Ans:(a)

The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Last Answer : Answer : D

Which of the following statement(s) is/are true concerning DNA? a. DNA is contained only in the nucleus of the cell b. DNA strands are encoded by the sequence of four bases—adenine, guanine, cytosine and uridine c. The basic unit of information of DNA is the intron, a sequence of three bases d. There are an infinite number of possible codons

Description : Which of the following statement(s) is/are true concerning DNA? a. DNA is contained only in the nucleus of the cell b. DNA strands are encoded by the sequence of four bases-adenine, guanine, cytosine ... DNA is the intron, a sequence of three bases d. There are an infinite number of possible codons

Last Answer : Answer: a The genetic blueprint of an organism is carried in the nucleus of every cell, encoded by the sequence of four bases-adenine, guanine, cytosine and thymine, which together make up ... codons. Sixty-one of these code for amino acids and three are termination signals called stop codons

DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these

Description : DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these

Last Answer : Answer : C

The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Last Answer : Answer : D

What rule is use to join the free nucleotides to the exposed bases of the DNA?

Description : What rule is use to join the free nucleotides to the exposed bases of the DNA?

Last Answer : watson-base pairing

What rule is use to join the free nucleotides to the exposed bases of the DNA?

Description : What rule is use to join the free nucleotides to the exposed bases of the DNA?

Last Answer : watson-base pairing

A segment of DNA has 120 adenine and 120 cytosine bases. The total number of nucleotides present in the segment is (a) 120 (b) 240 (c) 60 (d) 480.

Description : A segment of DNA has 120 adenine and 120 cytosine bases. The total number of nucleotides present in the segment is (a) 120 (b) 240 (c) 60 (d) 480.

Last Answer : (d) 480.

Which of the following statement is false (a). Enzymes are differentiated by electrophoresis method (b). While DNA sequencing both forward and reverse primers are used (c). Amplification is done through PCR (d). ABO blood grouping is mainly used for differentiating individuals

Description : Which of the following statement is false (a). Enzymes are differentiated by electrophoresis method (b). While DNA sequencing both forward and reverse primers are used (c). Amplification is done through PCR (d). ABO blood grouping is mainly used for differentiating individuals

Last Answer : Ans: (b). While DNA sequencing both forward and reverse primers are used 

Similarity in DNA and RNA is that (a) both are polymer of nucleotides (b) both have similar pyrimidine (c) both have similar sugar (d) both are genetic material.

Description : Similarity in DNA and RNA is that (a) both are polymer of nucleotides (b) both have similar pyrimidine (c) both have similar sugar (d) both are genetic material.

Last Answer : (b) both have similar pyrimidine

Similarity in DNA and RNA is that (a) both are polymer of nucleotides (b) both have similar pyrimidine (c) both have similar sugar (d) both are genetic material.

Description : Similarity in DNA and RNA is that (a) both are polymer of nucleotides (b) both have similar pyrimidine (c) both have similar sugar (d) both are genetic material.

Last Answer : both are polymer of nucleotides

The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin

Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin

Last Answer : Answer : B

Another name for reverse transcriptase is (A) DNA dependent DNA polymerase (B) DNA dependent RNA polymerase (C) RNA dependent DNA polymerase (D) RNA dependent RNA polymerase

Description : Another name for reverse transcriptase is (A) DNA dependent DNA polymerase (B) DNA dependent RNA polymerase (C) RNA dependent DNA polymerase (D) RNA dependent RNA polymerase

Last Answer : Answer : C

RNA directed DNA polymerase is (A) Replicase (B) Transcriptase (C) Reversetranscriptase (D) Polymerase–III

Description : RNA directed DNA polymerase is (A) Replicase (B) Transcriptase (C) Reversetranscriptase (D) Polymerase–III

Last Answer : Answer : C

DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III

Description : DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III

Last Answer : Answer : B

The antibiotic which inhibits DNA dependent RNA polymerase is (A) Mitomycin C (B) Actinomycin d (C) Streptomycin (D) Puromycin

Description : The antibiotic which inhibits DNA dependent RNA polymerase is (A) Mitomycin C (B) Actinomycin d (C) Streptomycin (D) Puromycin

Last Answer : Answer : B

α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase

Description : α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase

Last Answer : Answer : C

A consensus sequence on DNA, called TATA box, is the site for attachment of (A) RNA-dependent DNA polymerase (B) DNA-dependent RNA polymerase (C) DNA-dependent DNA polymerase (D) DNA topoisomerase II

Description : A consensus sequence on DNA, called TATA box, is the site for attachment of (A) RNA-dependent DNA polymerase (B) DNA-dependent RNA polymerase (C) DNA-dependent DNA polymerase (D) DNA topoisomerase II

Last Answer : Answer : B

The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Description : The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Last Answer : Answer : D

The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Description : The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Last Answer : Answer : C

DNA-dependent RNA polymerase is a (A) Monomer (B) Dimer (C) Trimer (D) Tetramer

Description : DNA-dependent RNA polymerase is a (A) Monomer (B) Dimer (C) Trimer (D) Tetramer

Last Answer : Answer : D

All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses

Description : All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses

Last Answer : Answer : D

Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these

Description : Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these

Last Answer : Answer : A

Deoxyribonucleotides are added to RNA primer by (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these

Description : Deoxyribonucleotides are added to RNA primer by (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these

Last Answer : Answer : C

RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III

Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III

Last Answer : Answer : B

The binding of prokaryotic DNA dependent RNA polymerase to promoter sites of genes is inhibited by the antibiotic: (A) Puromycin (B) Rifamycin (C) Terramycin (D) Streptomycin

Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sites of genes is inhibited by the antibiotic: (A) Puromycin (B) Rifamycin (C) Terramycin (D) Streptomycin

Last Answer : Answer : B

The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase

Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase

Last Answer : Answer : C

Which one of the following is not applicable to RNA? (a) Heterocyclic nitrogenous bases (b) Chargaff ’s rule (c) Complementary base pairing (d) 5’ phosphoryl and 3’ hydroxyl ends

Description : Which one of the following is not applicable to RNA? (a) Heterocyclic nitrogenous bases (b) Chargaff ’s rule (c) Complementary base pairing (d) 5’ phosphoryl and 3’ hydroxyl ends

Last Answer : (a) Heterocyclic nitrogenous bases

Which one of the following is not applicable to RNA? (a) Heterocyclic nitrogenous bases (b) Chargaff ’s rule (c) Complementary base pairing (d) 5’ phosphoryl and 3’ hydroxyl ends

Description : Which one of the following is not applicable to RNA? (a) Heterocyclic nitrogenous bases (b) Chargaff ’s rule (c) Complementary base pairing (d) 5’ phosphoryl and 3’ hydroxyl ends

Last Answer : (b) Chargaff ’s rule