Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.

Restriction endonucleases are
(a) used for in vitro DNA synthesis
(b) used in genetic engineering
(c) synthesized by bacteria
(d) present in mammalian cells for degradation of
DNA.
by

1 Answer

Answer :

(b) used in genetic engineering
by

Related questions

Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Description : Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Last Answer : (d) are synthesized by bacteria as part of their defense mechanism.

Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro

All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Last Answer : Answer : D

The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Last Answer : Answer : D

Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.

Description : Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.

Last Answer : b. Chemically Open Dna Molecules.

Restriction endonucleases are present in (A) Viruses (B) Bacteria (C) Eukaryotes (D) All of these

Description : Restriction endonucleases are present in (A) Viruses (B) Bacteria (C) Eukaryotes (D) All of these

Last Answer : Answer : B

In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Last Answer : Answer : C

Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Last Answer : Answer : A

Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids

Description : Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids

Last Answer : Answer : C

Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein

Description : Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein

Last Answer : Answer : B

Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Last Answer : Answer : D

Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Last Answer : (a) make cuts at specific positions within the DNA molecule

he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases

Description : he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases

Last Answer : (b) Restriction endonucleases

The DNA fragments produced by the use of restriction endonucleases can be separated by

Description : 4. The DNA fragments produced by the use of restriction endonucleases can be separated by (a) polymerase chain reaction (b) gel electrophoresis (c) density gradient centrifugation (d) any of the above.

Last Answer : gel electrophoresis

Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease

Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease

Last Answer : Answer : D

The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Last Answer : (a) they can cut DNA at specific base sequence

Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Last Answer : d) Sticky ends can be joined by using DNA ligases.

Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase

Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase

Last Answer : (a) restriction endonuclease

Do eukaryotic cells have restriction endonucleases? -Biology

Description : Do eukaryotic cells have restriction endonucleases? -Biology

Last Answer : answer:

Which one of the following is a case of wrong matching? (a) Somatic – Fusion of two hybridization diverse cells (b) Vector DNA – Site for tRNA synthesis (c) Micropropagation – In vitro production of plants in large numbers (d) Callus – Unorganised mass of cells produced in tissue culture

Description : Which one of the following is a case of wrong matching? (a) Somatic – Fusion of two hybridization diverse cells (b) Vector DNA – Site for tRNA synthesis (c) Micropropagation – In vitro production of plants in large numbers (d) Callus – Unorganised mass of cells produced in tissue culture

Last Answer : (b) Vector DNA – Site for tRNA synthesis

9. Which one of the following is a case of wrong matching ? (1) Callus - Unorganised mass of cells produced in tissue culture (2) Somatic hybridization - Fusion of two diverse cells (3) Vector DNA - Site for t-RNA synthesis (4) Micropropagation - In vitro production of plants in large number

Description : 9. Which one of the following is a case of wrong matching ? (1) Callus - Unorganised mass of cells produced in tissue culture (2) Somatic hybridization - Fusion of two diverse cells (3) Vector DNA - Site for t-RNA synthesis (4) Micropropagation - In vitro production of plants in large number

Last Answer : Vector DNA - Site for t-RNA synthesis

Assertion : Interferons are effective against viruses. Reason : Proteins which can be synthesized only by genetic engineering are effective against vi

Description : Assertion : Interferons are effective against viruses. Reason : Proteins which can be synthesized only by genetic engineering are effective against vi

Last Answer : Assertion : Interferons are effective against viruses. Reason : Proteins which can be synthesized only ... If both the assertion and reason are false

In mammalian cell cycle, synthesis of DNA occurs during (A) S phase (B) G1 phase (C) Mitotic Phase (D) G2 phase

Description : In mammalian cell cycle, synthesis of DNA occurs during (A) S phase (B) G1 phase (C) Mitotic Phase (D) G2 phase

Last Answer : Answer : A

All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non-coding regions of DNA (C) It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease

Description : All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non- ... It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease

Last Answer : Answer : D

PCR and restriction fragment length polymorphism are the methods for(a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Description : PCR and restriction fragment length polymorphism are the methods for (a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Last Answer : (d) genetic fingerprinting.

Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.

Description : Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.

Last Answer : (c) cuts the DNA molecule at specific sites

The promoter on an expression vector used to overproduce proteins in bacteria is A.a bacterial promoter B.expressed both in bacteria and mammalian cells C.not a regulated promoterD.the natural promoter of the gene being cloned

Description : The promoter on an expression vector used to overproduce proteins in bacteria is A.a bacterial promoter B.expressed both in bacteria and mammalian cells C.not a regulated promoter D.the natural promoter of the gene being cloned

Last Answer : A.a bacterial promoter

Trimethoprim inhibits bacteria without affecting mammalian cells because: A. It does not penetrate mammalian cells B. It has high affinity for bacterial but low affinity for mammalian dihydrofolate reductase enzyme C. It inhibits bacterial folate synthetase as well as dihydrofolate reductase enzymes D. All of the above

Description : Trimethoprim inhibits bacteria without affecting mammalian cells because: A. It does not penetrate mammalian cells B. It has high affinity for bacterial but low affinity for mammalian dihydrofolate ... bacterial folate synthetase as well as dihydrofolate reductase enzymes D. All of the above

Last Answer : B. It has high affinity for bacterial but low affinity for mammalian dihydrofolate reductase enzyme

The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.

Description : The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.

Last Answer : (c) DNA ligase

What Genetic engineering can be accomplished in bacteria using?

Description : What Genetic engineering can be accomplished in bacteria using?

Last Answer : Genetic engineering involves the use of recombinant DNA technology, the process by which a DNA sequence is manipulated in vitro, thus creating recombinant DNA molecules that have new combinations of genetic material

Production of a human protein in bacteria by genetic engineering is possible because (a) the human chromosome can replicate in bacterial cell (b) the mechanism of gene regulation is identical in humans and bacteria (c) bacterial cell can carry out the RNA splicing reactions (d) the genetic code is universal.

Description : Production of a human protein in bacteria by genetic engineering is possible because (a) the human chromosome can replicate in bacterial cell (b) the mechanism of gene regulation is identical in ... ) bacterial cell can carry out the RNA splicing reactions (d) the genetic code is universal.

Last Answer : (d) the genetic code is universal.

Two bacteria found to be very useful in genetic engineering experiments are (a) Nitrobacter and Azotobacter (b) Rhizobium and Diplococcus (c) Nitrosomonas and Klebsiella (d) Escherichia and Agrobacterium.

Description : Two bacteria found to be very useful in genetic engineering experiments are (a) Nitrobacter and Azotobacter (b) Rhizobium and Diplococcus (c) Nitrosomonas and Klebsiella (d) Escherichia and Agrobacterium.

Last Answer : (d) Escherichia and Agrobacterium.

Which one of the following bacteria has found extensive use in genetic engineering work in plants? (a) Clostridium septicum (b) Xanthomonas citri (c) Bacillus coagulens (d) Agrobacterium tumefaciens

Description : Which one of the following bacteria has found extensive use in genetic engineering work in plants? (a) Clostridium septicum (b) Xanthomonas citri (c) Bacillus coagulens (d) Agrobacterium tumefaciens

Last Answer : (d) Agrobacterium tumefaciens

The bacteria generally used for genetic engineering is (a) Agrobacterium (b) Bacillus (c) Pseudomonas (d) Clostridium.

Description : The bacteria generally used for genetic engineering is (a) Agrobacterium (b) Bacillus (c) Pseudomonas (d) Clostridium.

Last Answer : (a) Agrobacterium

Why do scientists use bacteria in genetic engineering?

Description : Why do scientists use bacteria in genetic engineering?

Last Answer : Bacterial cell is very simple and easy to handle for the scientists. It is easy for the scientists to isolate plasmid from a bacterial cell and attach a gene with it. Another reason is its fast rate of reproduction.

An antibiotic is - (1) achemical synthesized by a human cell against a microorganism (2) a chemical synthesised by amicro-organism against another micro-organisms (3) a substance produced by blood cells against bacteria (4) a substance produced by blood cells against infection.

Description : An antibiotic is - (1) achemical synthesized by a human cell against a microorganism (2) a chemical synthesised by amicro-organism against another micro-organisms (3) a substance produced by blood cells against bacteria (4) a substance produced by blood cells against infection.

Last Answer : (2) a chemical synthesised by amicro-organism against another microorganisms Explanation: An antibacterial is an agent that inhibits bacterial growth or kills bacteria. The term is often used ... by a microorganism that is antagonistic to the growth of other microorganisms in high dilution.

An antibiotic is (1) a chemical synthesized by a human cell against a microorganism (2) a chemical synthesised by a micro-organism against another micro-organisms (3) a substance produced by blood cells against bacteria (4) a substance produced by blood cells against infection.

Description : An antibiotic is (1) a chemical synthesized by a human cell against a microorganism (2) a chemical synthesised by a micro-organism against another micro-organisms (3) a substance produced by blood cells against bacteria (4) a substance produced by blood cells against infection.

Last Answer : a chemical synthesised by a micro-organism against another micro-organisms

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors. (i) Bacterium (ii) Plasmid (iii)Plasmodium (iv) Bacteriophage (a) (i), (ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)

Description : In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these ... ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)

Last Answer : (d) (ii) and (iv)

Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis

Description : Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis

Last Answer : (d) Zinc finger analysis

Genetic information of nuclear DNA is transmitted to the site of protein synthesis by (A) rRNA (B) mRNA (C) tRNA (D) Polysomes

Description : Genetic information of nuclear DNA is transmitted to the site of protein synthesis by (A) rRNA (B) mRNA (C) tRNA (D) Polysomes

Last Answer : D

.After a mutation at a genetic locus the character of an organism changes due to change in (a) protein structure (b) DNA replication (c) protein synthesis pattern (d) RNA transcription pattern.

Description : .After a mutation at a genetic locus the character of an organism changes due to change in (a) protein structure (b) DNA replication (c) protein synthesis pattern (d) RNA transcription pattern.

Last Answer : (d) RNA transcription pattern.

Which of the following is the correct sequence of events in the origin of life? I. Formation of protobionts II. Synthesis of organic monomers III. Synthesis of organic polymers IV. Formation of DNA-based genetic systems (a) I, II, III, IV (b) I, III, II, IV (c) II, III, I, IV (d) II, III, IV, I

Description : Which of the following is the correct sequence of events in the origin of life? I. Formation of protobionts II. Synthesis of organic monomers III. Synthesis of organic polymers IV. Formation of DNA-based genetic systems (a) I, II, III, IV (b) I, III, II, IV (c) II, III, I, IV (d) II, III, IV, I

Last Answer : c) II, III, I, IV

After a mutation at a genetic locus the character of an organism changes due to change in (a) protein structure (b) DNA replication (c) protein synthesis pattern (d) RNA transcription pattern.

Description : After a mutation at a genetic locus the character of an organism changes due to change in (a) protein structure (b) DNA replication (c) protein synthesis pattern (d) RNA transcription pattern.

Last Answer : a) protein structure

Which of the following structures present in mammalian skin directly helps in keeping the body warm? (1) Pigmented cells (2) Sweat glands (3) Lymph vessels (4) Blood capillaries

Description : Which of the following structures present in mammalian skin directly helps in keeping the body warm? (1) Pigmented cells (2) Sweat glands (3) Lymph vessels (4) Blood capillaries

Last Answer : (2) Sweat glands Explanation: In the skin, two types of glands are present.

Which of the following structures present in mammalian skin directly helps in keeping the body warm? (1) Pigmented cells (2) Sweat glands (3) Lymph vessels (4) Blood capillaries

Description : Which of the following structures present in mammalian skin directly helps in keeping the body warm? (1) Pigmented cells (2) Sweat glands (3) Lymph vessels (4) Blood capillaries

Last Answer : (3) Lymph vessels Explanation: The lymphatic system consists of a network of specialized lymphatic vessels and various tissues and organs throughout the body that contain lymphocytes (White Blood Cells) and ... skin and in the deeper fatty tissues near the muscles and can be found near arteries.

Which of the following structures present in mammalian skin directly helps in keeping the body warm ? (1) Pigmented cells (2) Sweat glands (3) Lymph vessels (4) Blood capillaries

Description : Which of the following structures present in mammalian skin directly helps in keeping the body warm ? (1) Pigmented cells (2) Sweat glands (3) Lymph vessels (4) Blood capillaries

Last Answer : Lymph vessels

Which of the following is true? (1) DNA is the genetic material in most of the organism (2) RNA is the genetic material in most viruses and bacteria (3) DNA is the genetic material in all the viruses (4) RNA is the genetic material in all the viruses

Description : Which of the following is true? (1) DNA is the genetic material in most of the organism (2) RNA is the genetic material in most viruses and bacteria (3) DNA is the genetic material in all the viruses (4) RNA is the genetic material in all the viruses

Last Answer : (1) DNA is the genetic material in most of the organism Explanation: Deoxyribonucleic acid (DNA) molecules are informational molecules encoding the genetic instructions used in the development and functioning of all ... letters. G, A, T, and C. DNA is well-suited for biological information storage.

Which of the following features of genetic code does allow bacteria to produce human insulin by recombinant DNA technology? (a) Genetic code is specific. (b) Genetic code is not ambiguous. (c) Genetic code is redundant. (d) Genetic code is nearly universal

Description : Which of the following features of genetic code does allow bacteria to produce human insulin by recombinant DNA technology? (a) Genetic code is specific. (b) Genetic code is not ambiguous. (c) Genetic code is redundant. (d) Genetic code is nearly universal

Last Answer : (c) Genetic code is redundant.

The Pneumococcus experiment proves that (a) bacteria do not reproduce sexually (b) RNA sometime controls the production of DNA and proteins (c) DNA is the genetic material (d) bacteria undergo binary fission.

Description : The Pneumococcus experiment proves that (a) bacteria do not reproduce sexually (b) RNA sometime controls the production of DNA and proteins (c) DNA is the genetic material (d) bacteria undergo binary fission.

Last Answer : (d) bacteria undergo binary fission.

Which of the following features of genetic code does allow bacteria to produce human insulin by recombinant DNA technology? (a) Genetic code is specific. (b) Genetic code is not ambiguous. (c) Genetic code is redundant. (d) Genetic code is nearly universal.

Description : Which of the following features of genetic code does allow bacteria to produce human insulin by recombinant DNA technology? (a) Genetic code is specific. (b) Genetic code is not ambiguous. (c) Genetic code is redundant. (d) Genetic code is nearly universal.

Last Answer : (d) Genetic code is nearly universal