In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping
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All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Last Answer : Answer : D

Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids

Description : Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids

Last Answer : Answer : C

Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein

Description : Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein

Last Answer : Answer : B

A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Last Answer : b. restriction endonuclease

Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Last Answer : d) Sticky ends can be joined by using DNA ligases.

Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) The enzyme cuts DNA molecule at identified position within the DNA. (c) The enzyme binds DNA at specific sites and cuts only one of the two strands. (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Last Answer : (d) ligase.

Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Last Answer : (a) make cuts at specific positions within the DNA molecule

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequence of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequence of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

During transcription, do both strands of DNA get transcribed into a double stranded RNA molecule?

Description : During transcription, do both strands of DNA get transcribed into a double stranded RNA molecule?

Last Answer : No. RNA is single stranded and is transcribed from only one of the DNA strands. DNA replication does make copies of both strands.

The DNA fragments produced by the use of restriction endonucleases can be separated by

Description : 4. The DNA fragments produced by the use of restriction endonucleases can be separated by (a) polymerase chain reaction (b) gel electrophoresis (c) density gradient centrifugation (d) any of the above.

Last Answer : gel electrophoresis

All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non-coding regions of DNA (C) It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease

Description : All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non- ... It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease

Last Answer : Answer : D

Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false

The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Last Answer : Answer : D

Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Last Answer : Answer : A

Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Last Answer : Answer : D

Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease

Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease

Last Answer : Answer : D

Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.

Description : Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.

Last Answer : (b) used in genetic engineering

Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Description : Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Last Answer : (d) are synthesized by bacteria as part of their defense mechanism.

Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro

he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases

Description : he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases

Last Answer : (b) Restriction endonucleases

Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.

Description : Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.

Last Answer : b. Chemically Open Dna Molecules.

Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.

Description : Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.

Last Answer : (b) restriction endonuclease

Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate

Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate

Last Answer : (a) E.coli restriction endonuclease I

Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.

Description : Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.

Last Answer : (c) cuts the DNA molecule at specific sites

Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase

Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase

Last Answer : (a) restriction endonuclease

Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Last Answer : a) Topoisomease II

Restriction endonucleases are present in (A) Viruses (B) Bacteria (C) Eukaryotes (D) All of these

Description : Restriction endonucleases are present in (A) Viruses (B) Bacteria (C) Eukaryotes (D) All of these

Last Answer : Answer : B

PCR and restriction fragment length polymorphism are the methods for(a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Description : PCR and restriction fragment length polymorphism are the methods for (a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Last Answer : (d) genetic fingerprinting.

In double helix of DNA, the two DNA strands are (a) coiled around a common axis (b) coiled around each other(c) coiled differently (d) coiled over protein sheath.

Description : In double helix of DNA, the two DNA strands are (a) coiled around a common axis (b) coiled around each other (c) coiled differently (d) coiled over protein sheath.

Last Answer : (a) coiled around a common axis

The unwound strands of DNA are held apart by (A) Single strand binding protein (B) Double strand binding protein (C) Rep protein (D) DNAA protein

Description : The unwound strands of DNA are held apart by (A) Single strand binding protein (B) Double strand binding protein (C) Rep protein (D) DNAA protein

Last Answer : Answer : A

The mitochondrial DNA is (A) Like the nuclear DNA in structure (B) Single stranded, linear (C) Double stranded, circular (D) Single stranded, circular

Description : The mitochondrial DNA is (A) Like the nuclear DNA in structure (B) Single stranded, linear (C) Double stranded, circular (D) Single stranded, circular

Last Answer : Answer : C

A plasmid is a (A) Single stranded linear DNA (B) Single stranded circular DNA (C) Double stranded linear DNA (D) Double stranded circular DNA

Description : A plasmid is a (A) Single stranded linear DNA (B) Single stranded circular DNA (C) Double stranded linear DNA (D) Double stranded circular DNA

Last Answer : Answer : D

Actinomycin D binds to (A) Double stranded DNA (B) Single stranded DNA (C) Single stranded RNA (D) DNA-RNA hybrid

Description : Actinomycin D binds to (A) Double stranded DNA (B) Single stranded DNA (C) Single stranded RNA (D) DNA-RNA hybrid

Last Answer : Answer : A

S1 nuclease hydrolyses (A) DNA of somatic cells (B) DNA of sperms (C) Any double stranded DNA (D) Any single stranded DNA

Description : S1 nuclease hydrolyses (A) DNA of somatic cells (B) DNA of sperms (C) Any double stranded DNA (D) Any single stranded DNA

Last Answer : Answer : D

Mitochondrial DNA is (A) Circular double stranded (B) Circular single stranded (C) Linear double helix (D) None of these

Description : Mitochondrial DNA is (A) Circular double stranded (B) Circular single stranded (C) Linear double helix (D) None of these

Last Answer : A

Do eukaryotic cells have restriction endonucleases? -Biology

Description : Do eukaryotic cells have restriction endonucleases? -Biology

Last Answer : answer:

Antiparallel strands of a DNA molecule means that (a) one strand turns clockwise (b) one strand turns anti-clockwise (c) the phosphate groups of two DNA strands, at their ends, share the same position (d) the phosphate groups at the start of two DNA strands are in opposite position (pole).

Description : Antiparallel strands of a DNA molecule means that (a) one strand turns clockwise (b) one strand turns anti-clockwise (c) the phosphate groups of two DNA strands, at their ends, share the same position (d) the phosphate groups at the start of two DNA strands are in opposite position (pole).

Last Answer : (d) the phosphate groups at the start of two DNA strands are in opposite position (pole).

Genophore/bacterial genome or nucleoid is made of (a) histones and non-histones(b) RNA and histones (c) a single double stranded DNA (d) a single stranded DNA.

Description : Genophore/bacterial genome or nucleoid is made of (a) histones and non-histones (b) RNA and histones (c) a single double stranded DNA (d) a single stranded DNA.

Last Answer : c) a single double stranded DNA

A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using

Description : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using

Last Answer : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant ... B. Ligase C. Eco RI D. Taq polymerase

What determines where a restriction endonuclease cuts? -Biology

Description : What determines where a restriction endonuclease cuts? -Biology

Last Answer : answer:

Function of restriction endonuclease in bacteria -Biology

Description : Function of restriction endonuclease in bacteria -Biology

Last Answer : answer:

What is restriction endonuclease used for? -Biology

Description : What is restriction endonuclease used for? -Biology

Last Answer : answer:

What is restriction endonuclease used for? -Biology

Description : What is restriction endonuclease used for? -Biology

Last Answer : answer:

There is a restriction endonuclease called EcoRI. What does “co” part in it stand for? (a) colon (b) coelom (c) coenzyme (d) coli

Description : There is a restriction endonuclease called EcoRI. What does “co” part in it stand for? (a) colon (b) coelom (c) coenzyme (d) coli

Last Answer : (d) coli

Which of the following is a restriction endonuclease? (a) DNase I (b) RNase (c) Hind II (d) Protease

Description : Which of the following is a restriction endonuclease? (a) DNase I (b) RNase (c) Hind II (d) Protease

Last Answer : (c) Hind II

Which complementary sequence would NOT be recognized by a restriction endonucLease?a. GAATTC CTTAAG b. AAGCTT TTCGAA c. GTCGAC CAGCTG d. AATTCCTTAAGG

Description : Which complementary sequence would NOT be recognized by a restriction endonucLease? a. GAATTC CTTAAG b. AAGCTT TTCGAA c. GTCGAC CAGCTG d. AATTCCTTAAGG

Last Answer : d. AATTCCTTAAGG

The two strands of the DNA double helix are held together by (a) Hydrogen bonds (b)C=C double bonds (c) Hydrophobic bonds (d) Peptide bonds

Description : The two strands of the DNA double helix are held together by (a) Hydrogen bonds (b)C=C double bonds (c) Hydrophobic bonds (d) Peptide bonds

Last Answer : Ans:(a)