Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Identify the wrong statement with regard to
restriction enzymes.
(a) Each restriction enzyme functions by inspecting
the length of a DNA sequence.
(b) They cut the strand of DNA at palindromic sites.
(c) They are useful in genetic engineering.
(d) Sticky ends can be joined by using DNA ligases.
by

1 Answer

Answer :

d) Sticky ends can be joined by using DNA ligases.
by

Related questions

Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) The enzyme cuts DNA molecule at identified position within the DNA. (c) The enzyme binds DNA at specific sites and cuts only one of the two strands. (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.

Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false

The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Last Answer : (a) they can cut DNA at specific base sequence

All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Last Answer : Answer : D

DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Last Answer : Answer : C

In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Last Answer : Answer : C

Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Last Answer : (a) make cuts at specific positions within the DNA molecule

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)

Description : Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)

Last Answer : (c)

Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro

The cutting of DNA at specific locations became possible with the discovery of (a) selectable markers (b) ligases (c) restriction enzymes (d) probes.

Description : The cutting of DNA at specific locations became possible with the discovery of (a) selectable markers (b) ligases (c) restriction enzymes (d) probes.

Last Answer : (c) restriction enzymes

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5′ _____ GAATTC _____ 3′ 3′ _____ CTTAAG _____ 5′ (a) Replication completed (b) Deletion mutation (c) Start codon at the 5′ end (d) Palindromic sequence of base pairs

Description : Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5′ _____ GAATTC _____ 3′ 3′ _____ CTTAAG _____ 5′ (a) ... completed (b) Deletion mutation (c) Start codon at the 5′ end (d) Palindromic sequence of base pairs

Last Answer : (d) Palindromic sequence of base pairs

he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases

Description : he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases

Last Answer : (b) Restriction endonucleases

Chimeric DNA (A) Is found in bacteriophages (B) Contains unrelated genes (C) Has no restriction sites (D) Is palindromic

Description : Chimeric DNA (A) Is found in bacteriophages (B) Contains unrelated genes (C) Has no restriction sites (D) Is palindromic

Last Answer : Answer : B

Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease

Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease

Last Answer : Answer : D

A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Last Answer : b. restriction endonuclease

PCR and restriction fragment length polymorphism are the methods for(a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Description : PCR and restriction fragment length polymorphism are the methods for (a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Last Answer : (d) genetic fingerprinting.

Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Last Answer : a) Topoisomease II

All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non-coding regions of DNA (C) It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease

Description : All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non- ... It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease

Last Answer : Answer : D

A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using

Description : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using

Last Answer : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant ... B. Ligase C. Eco RI D. Taq polymerase

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Last Answer : (d) ligase.

Stem and loop structures are A- proteins that help partially denatured enzymes to recover their native configuration B- structures in DNA caused by inverted repeats C- structures at the ends of linear eukaryotic DNA molecules D- the bonds between adjacent DNA nucleotides in the same strand

Description : Stem and loop structures are A- proteins that help partially denatured enzymes to recover their native configuration B- structures in DNA caused by inverted repeats C- structures at the ends of linear eukaryotic DNA molecules D- the bonds between adjacent DNA nucleotides in the same strand

Last Answer : structures in DNA caused by inverted repeats

Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Last Answer : Answer : A

Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.

Description : Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.

Last Answer : (b) used in genetic engineering

Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Description : Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Last Answer : (d) are synthesized by bacteria as part of their defense mechanism.

Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase

Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase

Last Answer : (a) restriction endonuclease

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors. (i) Bacterium (ii) Plasmid (iii)Plasmodium (iv) Bacteriophage (a) (i), (ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)

Description : In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these ... ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)

Last Answer : (d) (ii) and (iv)

Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Last Answer : Answer : D

What are the functions of DNA ligases? -Biology

Description : What are the functions of DNA ligases? -Biology

Last Answer : answer:

What are the functions of DNA ligases? -Biology

Description : What are the functions of DNA ligases? -Biology

Last Answer : answer:

Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein

Description : Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein

Last Answer : Answer : B

6. Which of the following DNA sequences would a Restriction enzyme recognize and cut? a. ATGCAC TACGTG b. GATATC CTATAG c. TAGATA ATCTAT d. AATATA TTATAT

Description : 6. Which of the following DNA sequences would a Restriction enzyme recognize and cut? a. ATGCAC TACGTG b. GATATC CTATAG c. TAGATA ATCTAT d. AATATA TTATAT

Last Answer : b. GATATC CTATAG

Which one of the following represents a palindromic sequence in DNA? (a) 5′ - GAATTC - 3′ (b) 5′ - CCAATG - 3′ 3′ - CTTAAG - 5′ 3′ - GAATCC - 5′ (c) 5′ - CATTAG - 3′ (d) 5′ - GATACC - 3′ 3′ - GATAAC - 5′ 3′ - CCTAAG - 5′

Description : Which one of the following represents a palindromic sequence in DNA? (a) 5′ - GAATTC - 3′ (b) 5′ - CCAATG - 3′ 3′ - CTTAAG - 5′ 3′ - GAATCC - 5′ (c) 5′ - CATTAG - 3′ (d) 5′ - GATACC - 3′ 3′ - GATAAC - 5′ 3′ - CCTAAG - 5′

Last Answer : (a) 5′ - GAATTC - 3′ 5′ - CCAATG - 3′ 3′ - CTTAAG - 5′

The sequence that controls the copy number of the linked DNA in the vector, is termed (a) selectable marker (b) Ori site (c) palindromic sequence (d) recognition site.

Description : The sequence that controls the copy number of the linked DNA in the vector, is termed (a) selectable marker (b) Ori site (c) palindromic sequence (d) recognition site.

Last Answer : (b) Ori site

At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase

Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase

Last Answer : d. Okazaki fragments; DNA ligase

Which of the following restriction enzymes produces blunt ends ?

Description : Which of the following restriction enzymes produces blunt ends ?

Last Answer : Which of the following restriction enzymes produces blunt ends ? A. Xho I B. Hind III C. Sal I D. Eco RV

Which of the following restriction enzymes produces blunt ends? (a) SalI (b) EcoRV (c) XhoI (d) HindIII

Description : Which of the following restriction enzymes produces blunt ends? (a) SalI (b) EcoRV (c) XhoI (d) HindIII

Last Answer : (b) EcoRV

An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix of DNA by an enzyme known as DNA polymerase b. The initial product of DNA transcription is called heterogeneous nuclear RNA which codes directly for proteins c. After processing is complete, the mRNA is exported from the nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete

A selectable marker is used to (a) help in eliminating the non-transformants, so that the transformants can be regenerated (b) identify the gene for a desired trait in an alien organism (c) select a suitable vector for transformation in a specific crop (d) mark a gene on a chromosome for isolation using restriction enzyme.

Description : A selectable marker is used to (a) help in eliminating the non-transformants, so that the transformants can be regenerated (b) identify the gene for a desired trait in an alien organism (c) ... in a specific crop (d) mark a gene on a chromosome for isolation using restriction enzyme.

Last Answer : (a) help in eliminating the non-transformants, so that the transformants can be regenerated

Which one is the incorrect statement with regard to the importance of pedigree analysis? (a) It confirms that DNA is the carrier of genetic information. (b) It helps to understand whether the trait in question is dominant or recessive. (c) It confirms that the trait is linked to one of the autosome. (d) It helps to trace the inheritance of a specific trait.

Description : Which one is the incorrect statement with regard to the importance of pedigree analysis? (a) It confirms that DNA is the carrier of genetic information. (b) It helps to understand whether the trait in ... linked to one of the autosome. (d) It helps to trace the inheritance of a specific trait.

Last Answer : (a) It confirms that DNA is the carrier of genetic information.

Antiparallel strands of a DNA molecule means that (a) one strand turns clockwise (b) one strand turns anti-clockwise (c) the phosphate groups of two DNA strands, at their ends, share the same position (d) the phosphate groups at the start of two DNA strands are in opposite position (pole).

Description : Antiparallel strands of a DNA molecule means that (a) one strand turns clockwise (b) one strand turns anti-clockwise (c) the phosphate groups of two DNA strands, at their ends, share the same position (d) the phosphate groups at the start of two DNA strands are in opposite position (pole).

Last Answer : (d) the phosphate groups at the start of two DNA strands are in opposite position (pole).

What are sticky ends in DNA and their significance? -Biology

Description : What are sticky ends in DNA and their significance? -Biology

Last Answer : answer:

Which of the following molecules forms lengths of DNA with ‘sticky ends’?

Description : 12 Which of the following molecules forms lengths of DNA with ‘sticky ends’? a) DNA ligase b) DNA polymerase c) RNA polymerase d) Restriction enzyme

Last Answer : ANS: d Restriction enzyme

The process of copying genetic information from one strand of DNA in to RNA is termed as (a) translation (b) transcription (c) replication (d) mutation

Description : The process of copying genetic information from one strand of DNA in to RNA is termed as (a) translation (b) transcription (c) replication (d) mutation

Last Answer : Ans:(b)

$ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and leading strand are formed due to replication f

Description : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and leading strand are formed due to replication f

Last Answer : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and ... wrong D. If both As and R are wrong.

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequence of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequence of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Enzymes which catalyse binding of two substrates by covalent bonds are known as (A) Lyases (B) Hydrolases (C) Ligases (D) Oxidoreductases

Description : Enzymes which catalyse binding of two substrates by covalent bonds are known as (A) Lyases (B) Hydrolases (C) Ligases (D) Oxidoreductases

Last Answer : Answer : C

What is the sequence of the bases on one DNA strand?

Description : What is the sequence of the bases on one DNA strand?

Last Answer : Feel Free to Answer

If the base sequence on a separated DNA strand is CGTAGG what will the base sequence on its complementary strand be?

Description : If the base sequence on a separated DNA strand is CGTAGG what will the base sequence on its complementary strand be?

Last Answer : What is the answer ?