Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends
Last Answer : Answer : D
Description : Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids
Last Answer : Answer : C
Description : Chimeric DNA (A) Is found in bacteriophages (B) Contains unrelated genes (C) Has no restriction sites (D) Is palindromic
Last Answer : Answer : B
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Description : Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)
Last Answer : (c)
Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA
Description : Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein
Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms
Description : Satellite DNA contains (A) Highly repetitive sequences (B) Moderately repetitive sequences (C) Non-repetitive sequences (D) DNA-RNA hybrids
Last Answer : Answer : A
Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping
Description : Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Description : Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Description : Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.
Last Answer : (b) used in genetic engineering
Description : Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.
Last Answer : (d) are synthesized by bacteria as part of their defense mechanism.
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope
Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro
Description : he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases
Last Answer : (b) Restriction endonucleases
Description : Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.
Last Answer : b. Chemically Open Dna Molecules.
Description : 4. The DNA fragments produced by the use of restriction endonucleases can be separated by (a) polymerase chain reaction (b) gel electrophoresis (c) density gradient centrifugation (d) any of the above.
Last Answer : gel electrophoresis
Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.
Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.
Last Answer : d) Sticky ends can be joined by using DNA ligases.
Description : Restriction endonucleases are present in (A) Viruses (B) Bacteria (C) Eukaryotes (D) All of these
Description : 6. Which of the following DNA sequences would a Restriction enzyme recognize and cut? a. ATGCAC TACGTG b. GATATC CTATAG c. TAGATA ATCTAT d. AATATA TTATAT
Last Answer : b. GATATC CTATAG
Description : Do eukaryotic cells have restriction endonucleases? -Biology
Last Answer : answer:
Description : .Expressed Sequence Tags (ESTs) refers to (a) novel DNA sequences (b) genes expressed as RNA (c) polypeptide expression (d) DNA polymorphism.
Last Answer : (b) genes expressed as RNA
Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase
Last Answer : d. Okazaki fragments; DNA ligase
Description : The role of molecular chaperones is to A-facilitate binding of ribosomes to mRNA B-degrade newly synthesized polypeptides that contain inaccurate sequences C-.facilitate binding of RNA polymerase to DNA D-aid a newly synthesized polypeptide in folding to its proper shape
Last Answer : -aid a newly synthesized polypeptide in folding to its proper shape
Last Answer : aid a newly synthesized polypeptide in folding to its proper shape
Description : Cistron is a) The coding sequence of DNA b) The functional unit of DNA molecule that codes for a particular gene product c) Intervening non coding sequence of DNA d) The sequences which are removed during RNA splicing.
Last Answer : b) The functional unit of DNA molecule that codes for a particular gene product c) Intervening non coding sequence of DNA
Description : What is Chimeric DNA? -Biology
Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase
Last Answer : b. restriction endonuclease
Description : All of the following statements about eukaryotic promoters are true except (A) They may be located upstream or down stream from the structural gene (B) They have two consensus sequences ( ... (D) Mutations in promoter region can decrease the efficiency of transcription of the structural gene
Description : All of the following statements about bacterial promoters are true except (A) They are smaller than eukaryotic promoters (B) They have two consensus sequences upstream from the transcription star site (C) ... is the site for attachment of RNA polymerase (D) TATA box has a high melting temperature
Description : Function of telomeres in nucleus is (a) poleward movement (b) to initiate the RNA synthesis (c) to seal the ends of chromosome (d) to recognise the homologous chromosome
Last Answer : (c) to seal the ends of chromosome
Description : What is the significance of recognition sequences of restriction enzymes? -Biology
Description : The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.
Last Answer : (c) DNA ligase
Description : What is palindromic DNA? -Biology
Description : What is the significance of palindromic DNA? -Biology
Description : Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5′ _____ GAATTC _____ 3′ 3′ _____ CTTAAG _____ 5′ (a) ... completed (b) Deletion mutation (c) Start codon at the 5′ end (d) Palindromic sequence of base pairs
Last Answer : (d) Palindromic sequence of base pairs
Description : Which one of the following represents a palindromic sequence in DNA? (a) 5′ - GAATTC - 3′ (b) 5′ - CCAATG - 3′ 3′ - CTTAAG - 5′ 3′ - GAATCC - 5′ (c) 5′ - CATTAG - 3′ (d) 5′ - GATACC - 3′ 3′ - GATAAC - 5′ 3′ - CCTAAG - 5′
Last Answer : (a) 5′ - GAATTC - 3′ 5′ - CCAATG - 3′ 3′ - CTTAAG - 5′
Description : The sequence that controls the copy number of the linked DNA in the vector, is termed (a) selectable marker (b) Ori site (c) palindromic sequence (d) recognition site.
Last Answer : (b) Ori site
Description : The genetic code operates via (A) The protein moiety of DNA (B) The base sequences of DNA (C) The nucleotide sequence of mRNA (D) The base sequence of tRNA
Description : DNA finger printing is based on the presence in DNA of (A) Constant number of tandem repeats (B) Varibale number of tandem repeats (C) Non-repititive sequences in each DNA (D) Introns in eukaryotic DNA
Description : Yeast artificial chromosome can be used to amplify DNA sequences of the size (A) Upto 10 kb (B) Upto 45 kb (C) Upto 100 kb (D) Upto 1,000 kb
Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases
Description : Besides structural genes that encode proteins, DNA contains some regulatory sequences which are known as (A) Operons (B) Cistrons (C) Cis-acting elements (D) Trans-acting factors
Description : RNA transcription is more complex in eukaryotes because it is first made as a primary RNA transcript that contains intron and exon sequences. What kind of modification must occur to produce a mature mRNA?
Last Answer : RNA splicing. Introns must be removed and exons are spliced together to form a mature RNA transcript.
Description : A bacterial colony containing DNA made up of 100% N15 nitrogen bases is allowed to replicate in a medium containing N14 bases. After one round of replication the result would be a) All individuals ... Only 50% individuals would be hybrids d) All individuals would have DNA made up of 100% N14
Last Answer : b) All individuals will be hybrids
Description : Antibodies produced by a single clone of myeloma cells are referred to as _____ antibodies. a. Chimeric b. Monoclonal c. Polyclonal d. Hybrid
Last Answer : b. Monoclonal
Description : All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non- ... It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease