Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping
Last Answer : Answer : C
Description : Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein
Last Answer : Answer : B
Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences
Last Answer : Answer : A
Description : Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids
Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.
Last Answer : d) Sticky ends can be joined by using DNA ligases.
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Description : Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)
Last Answer : (c)
Description : Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.
Last Answer : (b) used in genetic engineering
Description : Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.
Last Answer : (d) are synthesized by bacteria as part of their defense mechanism.
Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA
Last Answer : Answer : D
Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA
Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false
Description : Restriction endonucleases are present in (A) Viruses (B) Bacteria (C) Eukaryotes (D) All of these
Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms
Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope
Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro
Description : Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.
Last Answer : b. Chemically Open Dna Molecules.
Description : 6. Which of the following DNA sequences would a Restriction enzyme recognize and cut? a. ATGCAC TACGTG b. GATATC CTATAG c. TAGATA ATCTAT d. AATATA TTATAT
Last Answer : b. GATATC CTATAG
Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase
Last Answer : b. restriction endonuclease
Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.
Description : Chimeric DNA (A) Is found in bacteriophages (B) Contains unrelated genes (C) Has no restriction sites (D) Is palindromic
Description : The eukaryotic genome differs from the prokaryotic genome because (a) the DNA is complexed with histone in prokaryotes (b) the DNA is circular and single stranded in prokaryotes (c) repetitive sequences are present in eukaryotes (d) genes in the former case are organised into operons.
Last Answer : (b) the DNA is circular and single stranded in prokaryotes
Description : he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases
Last Answer : (b) Restriction endonucleases
Description : 4. The DNA fragments produced by the use of restriction endonucleases can be separated by (a) polymerase chain reaction (b) gel electrophoresis (c) density gradient centrifugation (d) any of the above.
Last Answer : gel electrophoresis
Description : All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non- ... It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease
Description : Which one of the folLowing statements is NOT true of the influenza viruses? a. They have a segmented genome. b. The genome is double-stranded DNA. c. The viruses have an envelope. d. There are three types of flu viruses
Last Answer : b. The genome is double-stranded DNA.
Description : n lysogeny, A- a bacteriophage transfers bacterial DNA B- bacteria take up double stranded DNA from the environment C- DNA-degrading enzymes in the extracellular medium would stop the process D- a bacteriophage genome is integrated into the bacterial genome
Last Answer : a bacteriophage genome is integrated into the bacterial genome
Description : All of the following statements about eukaryotic promoters are true except (A) They may be located upstream or down stream from the structural gene (B) They have two consensus sequences ( ... (D) Mutations in promoter region can decrease the efficiency of transcription of the structural gene
Description : All of the following statements about bacterial promoters are true except (A) They are smaller than eukaryotic promoters (B) They have two consensus sequences upstream from the transcription star site (C) ... is the site for attachment of RNA polymerase (D) TATA box has a high melting temperature
Description : In sea urchin DNA, which is double stranded, 17% of the bases were shown to be cytosine. The percentages of the other three bases expected to be present in this DNA are (a) G 17%, A 33%, T 33% (b) G 8.5%, A 50%, T 24.5% (c) G 34%, A 24.5%, T 24.5% (d) G 17%, A 16.5%, T 32.5%.
Last Answer : (a) G 17%, A 33%, T 33%
Description : The hereditary material present in the bacterium E.coli is (a) single-stranded DNA (b) double-stranded DNA (c) DNA (d) RNA.
Last Answer : (b) double-stranded DNA
Description : are single-stranded DNA molecules that can recognize and bind to a distinctive nucLeotide sequence of a pathogen. a. Prophages b. Plasmids c. Cloning vectors d. DNA probes
Last Answer : d. DNA probes
Description : The mitochondrial DNA is (A) Like the nuclear DNA in structure (B) Single stranded, linear (C) Double stranded, circular (D) Single stranded, circular
Description : A plasmid is a (A) Single stranded linear DNA (B) Single stranded circular DNA (C) Double stranded linear DNA (D) Double stranded circular DNA
Description : Actinomycin D binds to (A) Double stranded DNA (B) Single stranded DNA (C) Single stranded RNA (D) DNA-RNA hybrid
Description : S1 nuclease hydrolyses (A) DNA of somatic cells (B) DNA of sperms (C) Any double stranded DNA (D) Any single stranded DNA
Description : Mitochondrial DNA is (A) Circular double stranded (B) Circular single stranded (C) Linear double helix (D) None of these
Last Answer : A
Description : Do eukaryotic cells have restriction endonucleases? -Biology
Last Answer : answer:
Description : Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Description : Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Description : All of the following statements about nonsense codons are true except (A) They do not code for amino acids (B) They act as chain termination signals (C) They are identical in nuclear and mitochondrial DNA (D) They have no complementary anticodons
Description : What are sticky ends in DNA and their significance? -Biology
Description : 12 Which of the following molecules forms lengths of DNA with ‘sticky ends’? a) DNA ligase b) DNA polymerase c) RNA polymerase d) Restriction enzyme
Last Answer : ANS: d Restriction enzyme
Description : All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses
Description : If the total amount of adenine and thymine in a double stranded DNA is 45% the amount of guanine this DNA will be `:`
Last Answer : If the total amount of adenine and thymine in a double stranded DNA is 45% the amount of guanine this DNA will be ` ... ` B. `27.5%` C. `45%` D. `55%`
Description : ? X l74 has: (a) Single stranded DNA (b) Single stranded RNA (c) Double stranded RNA (d) Double stranded DNA
Last Answer : Ans. ((a))
Description : Most viruses that infect plant possess (a) single-stranded DNA (b) single-stranded RNA (c) double-stranded DNA and RNA (d) double - stranded RNA only
Last Answer : Ans:(b)
Description : AIDS virus has – (1) single-stranded RNA (2) double-stranded RNA (3) single-stranded DNA (4) double-stranded DNA
Last Answer : (1) single-stranded RNA Explanation: AIDS viruses have single stranded RNA. It is composed of two copies of positive single-stranded RNA that codes for the virus's nine genes enclosed by a ... 2,000 copies of the viral protein. The single-stranded RNA is tightly bound to nucleocapsid proteins.
Description : AIDS virus is----------- A. Single-stranded RNA (Answer) B. Single-stranded DNA C. Double-stranded RNA D. Double-stranded DNA
Last Answer : A. Single-stranded RNA (Answer)
Description : Human immuno deficiency virus (HIV) has a protein coat and a genetic material which is (a) double stranded RNA (b) double stranded DNA (c) single stranded DNA (d) single stranded RNA
Last Answer : (d) single stranded RNA.
Description : A DNA with unequal nitrogen bases would most probably be (a) single stranded (b) double stranded (c) triple stranded (d) four stranded
Last Answer : (a) single stranded