Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease

Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease
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Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase

Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase

Last Answer : (a) restriction endonuclease

Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Last Answer : d) Sticky ends can be joined by using DNA ligases.

Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) The enzyme cuts DNA molecule at identified position within the DNA. (c) The enzyme binds DNA at specific sites and cuts only one of the two strands. (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.

Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Last Answer : a) Topoisomease II

In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Last Answer : Answer : C

An enzyme which brings about lysis of bacterial cell wall is (A) Amylase (B) Lysozyme (C) Trypsin (D) Lipase

Description : An enzyme which brings about lysis of bacterial cell wall is (A) Amylase (B) Lysozyme (C) Trypsin (D) Lipase

Last Answer : Answer : B

An example of functional plasma enzyme is (A) Lipoprotein lipase (B) Amylase (C) Aminotransferase (D) Lactate dehydrogenase

Description : An example of functional plasma enzyme is (A) Lipoprotein lipase (B) Amylase (C) Aminotransferase (D) Lactate dehydrogenase

Last Answer : Answer : A

In acute pancreatitis, the enzyme raised in first five days is (A) Serum amylase (B) Serum lactic dehydrogenase (C) Urinary lipase (D) Urinary amylase

Description : In acute pancreatitis, the enzyme raised in first five days is (A) Serum amylase (B) Serum lactic dehydrogenase (C) Urinary lipase (D) Urinary amylase

Last Answer : Answer : A

The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Last Answer : (a) they can cut DNA at specific base sequence

Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.

Description : Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.

Last Answer : (b) used in genetic engineering

Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Description : Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Last Answer : (d) are synthesized by bacteria as part of their defense mechanism.

Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro

Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.

Description : Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.

Last Answer : (b) restriction endonuclease

Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate

Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate

Last Answer : (a) E.coli restriction endonuclease I

Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.

Description : Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.

Last Answer : (c) cuts the DNA molecule at specific sites

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Last Answer : (d) ligase.

A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Last Answer : b. restriction endonuclease

Choose the correct enzyme - substrate pair. (a) Carbohydrate - Lipase (b) Maltase - Lactose (c) Rennin - Casein (d) Protein - Amylase

Description : Choose the correct enzyme - substrate pair. (a) Carbohydrate - Lipase (b) Maltase - Lactose (c) Rennin - Casein (d) Protein - Amylase

Last Answer : (c) Rennin - Casein

Which one of the following is the correct matching of the site of action on the given substrate, the enzyme acting upon it and the end product? (a) Small intestine : Proteins Pepsin  →  Amino acids (b) Stomach : Fats Lipase  →  Micelles (c) Duodenum : Triglycerides Trypsin  →  Monoglycerides (d) Small intestine : Starch α-Amylase  →  Disaccharide (maltose)

Description : Which one of the following is the correct matching of the site of action on the given substrate, the enzyme acting upon it and the end product? (a) Small intestine : Proteins Pepsin  →  ... Trypsin  →  Monoglycerides (d) Small intestine : Starch α-Amylase  →  Disaccharide (maltose)

Last Answer : (d) Small intestine : Starch α-Amylase  →  Disaccharide

Which of the following options best represents the enzyme composition of pancreatic juice? (a) Amylase, Pepsin, Trypsinogen, Maltase (b) Peptidase, Amylase, Pepsin, Rennin (c) Lipase, Amylase, Trypsinogen, Procarbo- xypeptidase (d) Amylase, Peptidase, Trypsinogen, Rennin

Description : Which of the following options best represents the enzyme composition of pancreatic juice? (a) Amylase, Pepsin, Trypsinogen, Maltase (b) Peptidase, Amylase, Pepsin, Rennin (c) Lipase, Amylase, Trypsinogen, Procarbo- xypeptidase (d) Amylase, Peptidase, Trypsinogen, Rennin

Last Answer : (c) Lipase, Amylase, Trypsinogen, Procarbo- xypeptidase

An enzyme that can stimulate germination of barley seeds is (a) invertase (b) α-amylase (c) lipase (d) protease.

Description : An enzyme that can stimulate germination of barley seeds is (a) invertase (b) α-amylase (c) lipase (d) protease.

Last Answer : (b) α-amylase

Pancreatic juice contains more than one enzyme. Which among the following combination is correct ? (1) Pepsin and Lipase (2) Amylase and Pepsin (3) Pepsin and Trypsin (4) Trypsin and Lipase

Description : Pancreatic juice contains more than one enzyme. Which among the following combination is correct ? (1) Pepsin and Lipase (2) Amylase and Pepsin (3) Pepsin and Trypsin (4) Trypsin and Lipase

Last Answer : 4) Trypsin and Lipase

Which of the following options best represents the enzyme composition of pancreatic juice ? (1) amylase, pepsin, trypsinogen, maltase (2) peptidase, amylase, pepsin, rennin (3) lipase, amylase, trypsinogen, procarboxypeptidase (4) amylase, peptidase, trypsinogen, rennin

Description : Which of the following options best represents the enzyme composition of pancreatic juice ? (1) amylase, pepsin, trypsinogen, maltase (2) peptidase, amylase, pepsin, rennin (3) lipase, amylase, trypsinogen, procarboxypeptidase (4) amylase, peptidase, trypsinogen, rennin

Last Answer : (3) lipase, amylase, trypsinogen, procarboxypeptidase

The enzyme which is used for the digestion of fat is (a) Water (b) Bile (c) Amylase (d) Lipase

Description : The enzyme which is used for the digestion of fat is (a) Water (b) Bile (c) Amylase (d) Lipase

Last Answer : (d) Lipase

The enzyme for starch digestion (a) Maltase (b) Invertase (c) Lipase (d) Amylase

Description : The enzyme for starch digestion (a) Maltase (b) Invertase (c) Lipase (d) Amylase

Last Answer : (d) Amylase

Zinc is a cofactor for (A) Acid phosphatase (B) Alkaline phosphatase (C) Amylase (D) Lipase

Description : Zinc is a cofactor for (A) Acid phosphatase (B) Alkaline phosphatase (C) Amylase (D) Lipase

Last Answer : Answer : B

The best test for acute pancreatitis in the presence of mumps is (A) A serological test for mumps (B) Serum amylase (C) Urinary amylase (D) Serum lipase

Description : The best test for acute pancreatitis in the presence of mumps is (A) A serological test for mumps (B) Serum amylase (C) Urinary amylase (D) Serum lipase

Last Answer : Answer : D

The most useful test for the diagnosis of acute hemorrhagic pancreatitis during the first few days is (A) Urinary lipase test (B) Serum calcium (C) Urinary amylase (D) Serum amylase

Description : The most useful test for the diagnosis of acute hemorrhagic pancreatitis during the first few days is (A) Urinary lipase test (B) Serum calcium (C) Urinary amylase (D) Serum amylase

Last Answer : Answer : D

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? (a) Lipase (b) Protease (c) Endonuclease (d) Exonuclease

Description : Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? (a) Lipase (b) Protease (c) Endonuclease (d) Exonuclease

Last Answer : (c) Endonuclease

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? (a) Lipase (b) Protease (c) Endonuclease (d) Exonuclease

Description : Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? (a) Lipase (b) Protease (c) Endonuclease (d) Exonuclease

Last Answer : c) G + C / A + T

All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non-coding regions of DNA (C) It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease

Description : All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non- ... It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease

Last Answer : Answer : D

What determines where a restriction endonuclease cuts? -Biology

Description : What determines where a restriction endonuclease cuts? -Biology

Last Answer : answer:

Function of restriction endonuclease in bacteria -Biology

Description : Function of restriction endonuclease in bacteria -Biology

Last Answer : answer:

What is restriction endonuclease used for? -Biology

Description : What is restriction endonuclease used for? -Biology

Last Answer : answer:

What is restriction endonuclease used for? -Biology

Description : What is restriction endonuclease used for? -Biology

Last Answer : answer:

There is a restriction endonuclease called EcoRI. What does “co” part in it stand for? (a) colon (b) coelom (c) coenzyme (d) coli

Description : There is a restriction endonuclease called EcoRI. What does “co” part in it stand for? (a) colon (b) coelom (c) coenzyme (d) coli

Last Answer : (d) coli

Which of the following is a restriction endonuclease? (a) DNase I (b) RNase (c) Hind II (d) Protease

Description : Which of the following is a restriction endonuclease? (a) DNase I (b) RNase (c) Hind II (d) Protease

Last Answer : (c) Hind II

Which complementary sequence would NOT be recognized by a restriction endonucLease?a. GAATTC CTTAAG b. AAGCTT TTCGAA c. GTCGAC CAGCTG d. AATTCCTTAAGG

Description : Which complementary sequence would NOT be recognized by a restriction endonucLease? a. GAATTC CTTAAG b. AAGCTT TTCGAA c. GTCGAC CAGCTG d. AATTCCTTAAGG

Last Answer : d. AATTCCTTAAGG

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequence of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequence of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Last Answer : Answer : D

Which one of the following pairs is wrongly matched? (a) Alcohol - Nitrogenase (b) Fruit juice - Pectinase (c) Textile - Amylase (d) Detergents - Lipase

Description : Which one of the following pairs is wrongly matched? (a) Alcohol - Nitrogenase (b) Fruit juice - Pectinase (c) Textile - Amylase (d) Detergents - Lipase

Last Answer : (a) Alcohol - Nitrogenase

Gastric juice of infants contains (a) pepsinogen, lipase, rennin (b) amylase, rennin, pepsinogen (c) maltase, pepsinogen, rennin (d) nuclease, pepsinogen, lipase

Description : Gastric juice of infants contains (a) pepsinogen, lipase, rennin (b) amylase, rennin, pepsinogen (c) maltase, pepsinogen, rennin (d) nuclease, pepsinogen, lipase

Last Answer : (a) pepsinogen, lipase, rennin

Some proteolytic enzymes are (C.P.M.T. 77) (a) Trypsin, peptidase, pepsin (b) Amylopsin, steapsin, ptyalin (c) Amylase, lipase, zymase (d) Urease, zymase, dehydrogenase

Description : Some proteolytic enzymes are (C.P.M.T. 77) (a) Trypsin, peptidase, pepsin (b) Amylopsin, steapsin, ptyalin (c) Amylase, lipase, zymase (d) Urease, zymase, dehydrogenase

Last Answer : (a) Trypsin, peptidase, pepsin

Which of the following converts peptones, proteoses and polypeptides into amino acids : (a) Amylase (b) Trypsin (c) Lipase (d) Rennin

Description : Which of the following converts peptones, proteoses and polypeptides into amino acids : (a) Amylase (b) Trypsin (c) Lipase (d) Rennin

Last Answer : (b) Trypsin

Emulsified fat is acted upon by : (a) Lipase (b) Amylase (c) Rennin (d) Pepsin

Description : Emulsified fat is acted upon by : (a) Lipase (b) Amylase (c) Rennin (d) Pepsin

Last Answer : (a) Lipase

Which of the following human digestive enzymes is incorrectly matched to its substrate? a) pepsin - protein b) trypsin - starch (pron: TRIP-sin) c) pancreatic amylase - starch (pron: AM-i-lase) d) lipase - fat e) maltase - maltose.

Description : Which of the following human digestive enzymes is incorrectly matched to its substrate? a) pepsin - protein b) trypsin - starch (pron: TRIP-sin) c) pancreatic amylase - starch (pron: AM-i-lase) d) lipase - fat e) maltase - maltose.

Last Answer : ANSWER: B -- TRYPSIN - STARCH

PCR and restriction fragment length polymorphism are the methods for(a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Description : PCR and restriction fragment length polymorphism are the methods for (a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Last Answer : (d) genetic fingerprinting.

Catalytic activity of salivary amylase requires the presence of (A) Chloride ions (B) Bromide ions (C) Iodide ions (D) All of these

Description : Catalytic activity of salivary amylase requires the presence of (A) Chloride ions (B) Bromide ions (C) Iodide ions (D) All of these

Last Answer : A