Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.
Last Answer : d) Sticky ends can be joined by using DNA ligases.
Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase
Last Answer : d. Okazaki fragments; DNA ligase
Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends
Last Answer : Answer : D
Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences
Last Answer : Answer : A
Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.
Description : Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)
Last Answer : (c)
Description : Chimeric DNA (A) Is found in bacteriophages (B) Contains unrelated genes (C) Has no restriction sites (D) Is palindromic
Last Answer : Answer : B
Description : Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5′ _____ GAATTC _____ 3′ 3′ _____ CTTAAG _____ 5′ (a) ... completed (b) Deletion mutation (c) Start codon at the 5′ end (d) Palindromic sequence of base pairs
Last Answer : (d) Palindromic sequence of base pairs
Description : Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Description : A bacterial colony containing DNA made up of 100% N15 nitrogen bases is allowed to replicate in a medium containing N14 bases. After one round of replication the result would be a) All individuals ... Only 50% individuals would be hybrids d) All individuals would have DNA made up of 100% N14
Last Answer : b) All individuals will be hybrids
Description : All of the following statements about bacterial promoters are true except (A) They are smaller than eukaryotic promoters (B) They have two consensus sequences upstream from the transcription star site (C) ... is the site for attachment of RNA polymerase (D) TATA box has a high melting temperature
Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase
Last Answer : d. RNA polymerase
Description : Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA
Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false
Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms
Description : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and leading strand are formed due to replication f
Last Answer : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and ... wrong D. If both As and R are wrong.
Description : After formation of replication fork (A) Both the new strands are synthesized discontinuously (B) One strand is synthesized continuously and the other discontinuously (C) Both the new strands are synthesized continuously (D) RNA primer is required only for the synthesis of one new strand
Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?
Last Answer : use restriction enzymes is the right answer for apex :)
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis
Last Answer : (d) Zinc finger analysis
Description : In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core ... RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation
Last Answer : Answer : C
Description : The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Description : The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases
Description : Termination of the synthesis of the RNA molecule is signaled by a sequence in the template strand of the DNA molecule, a signal that is recognized by a termination protein, the (A) Rho (ρ) factor (B) σ factor (C) δ factor (D) ε factor
Description : Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.
Last Answer : b. Chemically Open Dna Molecules.
Description : What is the significance of recognition sequences of restriction enzymes? -Biology
Last Answer : answer:
Description : Identify the correct statement with regard to G1 phase (Gap 1) of interphase. (a) DNA synthesis or replication takes place. (b) Reorganisation of all cell components takes place. (c) Cell is metabolically active, grows but does not replicate its DNA. (d) Nuclear division takes place.
Last Answer : (c) Cell is metabolically active, grows but does not
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes
Description : Replication of nuclear DNA in mammals is catalysed by (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase III
Description : 3’→5’ Exonuclease activity of DNA polymerase I (A) Removes ribonucleotides (B) Adds deoxyribonucleotides (C) Corrects errors in replication (D) Hydrolyses DNA into mononucleotides
Description : In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase
Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand
Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete
Description : Proto-oncogenes are: B A. DNA sequences in cells that are oncogenes themselves B. Cellular copies of genes that were first found in oncogenic retroviruses C. DNA viral sequences that are known to infect human cells D. Bacterial DNA counterparts
Last Answer : Cellular copies of genes that were first found in oncogenic retroviruses
Description : 6. Which of the following DNA sequences would a Restriction enzyme recognize and cut? a. ATGCAC TACGTG b. GATATC CTATAG c. TAGATA ATCTAT d. AATATA TTATAT
Last Answer : b. GATATC CTATAG
Description : What molecular structure is used to insert foreign DNA into a recipient bacterial cell that will replicate the foreign DNA -Biology
Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease
Last Answer : a) Topoisomease II
Description : The role of molecular chaperones is to A-facilitate binding of ribosomes to mRNA B-degrade newly synthesized polypeptides that contain inaccurate sequences C-.facilitate binding of RNA polymerase to DNA D-aid a newly synthesized polypeptide in folding to its proper shape
Last Answer : -aid a newly synthesized polypeptide in folding to its proper shape
Last Answer : aid a newly synthesized polypeptide in folding to its proper shape
Description : $ The regular sequences of a cell cycle is `G_(1),S,G_(2),M` and C ! Replication of DNA occurs during S phase.
Last Answer : $ The regular sequences of a cell cycle is `G_(1),S,G_(2),M` and C ! Replication of DNA occurs during ... , R is wrong D. If both As and R are wrong.
Description : Spontaneous mutations could arise from a. DNA replication errors. b. Atmospheric Radiation. c. Addition Of Insertion Sequences. d. All the above (A—C) are correct.
Last Answer : d. All the above (A—C) are correct.
Description : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the
Last Answer : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the ... Antistrand C. Template strand D. Coding strand
Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand
Last Answer : (a) template strand
Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand.
Description : The process of copying genetic information from one strand of DNA in to RNA is termed as (a) translation (b) transcription (c) replication (d) mutation
Last Answer : Ans:(b)
Description : During DNA replication, Okazaki fragments are used to elongate (a) the lagging strand towards replication fork (b) the leading strand away from replication fork (c) the lagging strand away from the replication fork (d) the leading strand towards replication fork.
Last Answer : (b) the leading strand away from replication fork
Description : In the semiconservative method of replication a. Both parent strands are degraded. b. One parent strand is conserved in each of the the new dna. c. Both parent strands reform with one another. d. Okazaki fragments form both of the new molecules of DNA.
Last Answer : b. One parent strand is conserved in each of the the new dna.