Define: i) Sterilization ii) Disinfection Classify different methods of sterilization.

1 Answer

Answer :

i) Sterilization: Sterilization is a process of complete destruction of all micro-organisms & their spores present in a system.

ii) Disinfection: A process that removes the infection potential by destroying micro-organisms but not generally bacterial spores.

Methods of sterilization:

A) Physical Methods:

1) Dry heat sterilization

2) Moist heat sterilization

3) Radiation sterilization

a) Use of UV rays

b) Ionising radiation

B) Chemical Methods:

1) Sterilization by heating with bactericide

2) Gaseous sterilization

C) Mechanical Methods: Sterilization involves the filtration of Parenteral preparations through the following bacteria proof filters:

1) Ceramic filters

2) Seitz filters

3) Sintered glass filters

4) Sintered metal filters

5) Membrane filters

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