Give the significance of sterilization using bactericidal solution, explain the method and name the bactericidal agents.

Give the significance of sterilization using bactericidal solution, explain the method and name the bactericidal agents.
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Answer :

Significance: The lethal effect of bactericide increases with the rise of temperature. This method is used for sterilizing aqueous preparation, which is unstable at the higher temperature, hence moist heat sterilization is not applicable method for sterilization. It is official in British Pharmacopoeia and Indian Pharmacopoeia. 


Method 

In this process the medicament is dissolved or suspended in a suitable solution of following bactericidal, as given in table, then preparation is sealed in final container and heated at 98°-100°C for 30 minutes in boiling water. IP 1985 permitted the use of following bactericides


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