Describe dry heat method of sterilization in detail.

Describe dry heat method of sterilization in detail.
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1 Answer

Answer :

Principle:

All the microorganism including spores are destroyed.

Principle of killing is by dehydration and oxidation of essential metabolites.

In hot air oven heating is done at 1600 C for 2 hours.

Construction:

It consists of double walled chamber made of steel.

Insulation is given of asbestos or other material for preventing heat loss.

The door is also double walled having insulation.

Two perforated shells provided to keep the material.

An electric fan is provided for uniform circulation of hot air.

A heater is fitted at the bottom for heating.

A thermometer for maintaining the temperature.

Working:

Wrap the material with paper.

Keep the Wrapped material in perforated shelves.

Material should not be kept at floor of the oven.

Close the door.

Switch on the oven and set the temperature and time as required.

After time is over.

Switch off the oven.

Allow to cool.

Take out the material. 

Diagram:

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