Description : How are restriction enzymes used to make recombinant DNA? -Biology
Last Answer : answer:
Description : Role of restriction enzymes and DNA ligase -Biology
Description : Why are restriction enzymes used for DNA fingerprinting? -Biology
Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA
Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false
Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?
Last Answer : use restriction enzymes is the right answer for apex :)
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Last Answer : Answer : C
Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms
Last Answer : Answer : D
Description : Read the following four statements (A-D) about certain mistakes in two of them. (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA ... mistakes? (a) B and C (b) C and D (c) A and C (d) A and B
Last Answer : (d) A and B
Description : PCR and restriction fragment length polymorphism are the methods for (a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.
Last Answer : (d) genetic fingerprinting.
Description : During the process of isolation of DNA, chilled ethanol is added to (a) precipitate DNA (b) break open the cell to release DNA (c) facilitate action of restriction enzymes (d) remove proteins such as histones.
Last Answer : a) precipitate DNA
Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.
Last Answer : (a) they can cut DNA at specific base sequence
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.
Last Answer : d) Sticky ends can be joined by using DNA ligases.
Description : The cutting of DNA at specific locations became possible with the discovery of (a) selectable markers (b) ligases (c) restriction enzymes (d) probes.
Last Answer : (c) restriction enzymes
Description : Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis
Last Answer : (d) Zinc finger analysis
Description : how is dna methylation used in dna repair? -General Knowledge
Last Answer : The mismatch-repair enzymes can use a lack of methylation to identify and remove newly synthesized DNA.
Description : Name some restriction enzymes. -Biology
Description : What is the significance of recognition sequences of restriction enzymes? -Biology
Description : Nomenclature of restriction enzymes. -Biology
Description : What is the enzymatic function of restriction enzymes? -Biology
Description : What is the source of restriction enzymes? -Biology
Description : What are restriction enzymes used for? -Biology
Description : Why are restriction enzymes called molecular scissors? -Biology
Description : Why is cutting enzymes known as the restriction enzymes? -Biology
Description : Which of the following restriction enzymes produces blunt ends ?
Last Answer : Which of the following restriction enzymes produces blunt ends ? A. Xho I B. Hind III C. Sal I D. Eco RV
Description : What role do restriction enzymes play in analysis of Edna?
Last Answer : Feel Free to Answer
Description : Restriction enzymes have been found in (A) Humans (B) Birds (C) Bacteria (D) Bacteriophase
Description : Which of the following restriction enzymes produces blunt ends? (a) SalI (b) EcoRV (c) XhoI (d) HindIII
Last Answer : (b) EcoRV
Description : The most important mechanism of antiinflammatory action of glucocorticoids is: A. Inhibition of lysosomal enzymes B. Restriction of recruitment of inflammatory cells at the site of inflammation C. Antagonism of action of interleukins D. Suppression of complement function
Last Answer : B. Restriction of recruitment of inflammatory cells at the site of inflammation
Description : Restriction enzymes are used in one of these techniques: (a). Sequencing (b). Genotyping (c). RFLP (d). Polymerization
Last Answer : Ans: (c). RFLP
Description : How does the restriction enzyme prevent the cutting of the endogenous DNA? -Biology
Description : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using
Last Answer : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant ... B. Ligase C. Eco RI D. Taq polymerase
Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA
Description : All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non- ... It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease
Description : Chimeric DNA (A) Is found in bacteriophages (B) Contains unrelated genes (C) Has no restriction sites (D) Is palindromic
Last Answer : Answer : B
Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping
Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends
Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences
Last Answer : Answer : A
Description : Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids
Description : Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein
Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease
Description : Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.
Last Answer : (b) used in genetic engineering
Description : Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.
Last Answer : (b) restriction endonuclease
Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate
Last Answer : (a) E.coli restriction endonuclease I
Description : Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.
Last Answer : (d) are synthesized by bacteria as part of their defense mechanism.
Description : Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.
Last Answer : (c) cuts the DNA molecule at specific sites
Description : Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)
Last Answer : (c)