A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?
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use restriction enzymes is the right answer for apex :)
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A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Last Answer : use restriction enzymes is the right answer for apex :)

A scientist wants to begin making a DNA fingerprint but she has only a very small DNA sample what should her next step be?

Description : A scientist wants to begin making a DNA fingerprint but she has only a very small DNA sample what should her next step be?

Last Answer : do a polymeras chain reaction (PCR). apex

Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis

Description : Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis

Last Answer : (d) Zinc finger analysis

DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Description : DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Last Answer : (a) electrophoresis

Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Last Answer : (a) make cuts at specific positions within the DNA molecule

DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Last Answer : Answer : C

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Last Answer : (d) ligase.

A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Last Answer : b. restriction endonuclease

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Last Answer : Answer : C

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Last Answer : Answer : C

In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Last Answer : Answer : D

The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

Description : The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

Last Answer : Answer : C

Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Last Answer : Answer : A

The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for both antigen and antibody detection b. Southern, northern, and western immunoblot techniques are used to detect DNA, RNA, or proteins, respectively c. Polymerase chain reaction (PCR) is a sensitive assay used to detect small amounts of microbial DNA, thus detecting infection at its early stages d. Infectious agents currently detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 5 3 5 3 5 3 5 3 5 5 3 3 5 3 5 3 Region to be amplified . (a) B - denaturation at a temperature of about 98°C separating the two DNA strands (b) A - denaturation at a temperature of about 50°C (c) C - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Description : The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 ... - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Last Answer : (c) C - extension in the presence of heat stable DNA polymerase

What should her next step be a scientist is interested in designing an alternative cancer treatment involving a less harmful type of radiation?

Description : What should her next step be a scientist is interested in designing an alternative cancer treatment involving a less harmful type of radiation?

Last Answer : Look at how radiation interacts with cancer cells

How are restriction enzymes used to make recombinant DNA? -Biology

Description : How are restriction enzymes used to make recombinant DNA? -Biology

Last Answer : answer:

Role of restriction enzymes and DNA ligase -Biology

Description : Role of restriction enzymes and DNA ligase -Biology

Last Answer : answer:

Restriction enzymes and DNA methylation -Biology

Description : Restriction enzymes and DNA methylation -Biology

Last Answer : answer:

Why are restriction enzymes used for DNA fingerprinting? -Biology

Description : Why are restriction enzymes used for DNA fingerprinting? -Biology

Last Answer : answer:

Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false

Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Last Answer : Answer : D

Read the following four statements (A-D) about certain mistakes in two of them. (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA from other macromolecules. (C) Downstream processing is one of the steps of rDNA technology. (D) Disarmed pathogen vectors are also used in transfer of rDNA into the host. Which of the two statements have mistakes? (a) B and C (b) C and D (c) A and C (d) A and B

Description : Read the following four statements (A-D) about certain mistakes in two of them. (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA ... mistakes? (a) B and C (b) C and D (c) A and C (d) A and B

Last Answer : (d) A and B

PCR and restriction fragment length polymorphism are the methods for(a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Description : PCR and restriction fragment length polymorphism are the methods for (a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.

Last Answer : (d) genetic fingerprinting.

During the process of isolation of DNA, chilled ethanol is added to (a) precipitate DNA (b) break open the cell to release DNA (c) facilitate action of restriction enzymes (d) remove proteins such as histones.

Description : During the process of isolation of DNA, chilled ethanol is added to (a) precipitate DNA (b) break open the cell to release DNA (c) facilitate action of restriction enzymes (d) remove proteins such as histones.

Last Answer : a) precipitate DNA

The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Last Answer : (a) they can cut DNA at specific base sequence

Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Last Answer : d) Sticky ends can be joined by using DNA ligases.

The cutting of DNA at specific locations became possible with the discovery of (a) selectable markers (b) ligases (c) restriction enzymes (d) probes.

Description : The cutting of DNA at specific locations became possible with the discovery of (a) selectable markers (b) ligases (c) restriction enzymes (d) probes.

Last Answer : (c) restriction enzymes

Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease

Description : Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease

Last Answer : a) Ligase

The following are true about DNA synthesis: a. it requires DNA polymerase b. reverse transcriptase enzymes are involved c. moves in a 5'---> 3' direction d. the rate of error in DNA synthesis is 1 in 105 base pairs

Description : The following are true about DNA synthesis: a. it requires DNA polymerase b. reverse transcriptase enzymes are involved c. moves in a 5'---> 3' direction d. the rate of error in DNA synthesis is 1 in 105 base pairs

Last Answer : it requires DNA polymerase

In addition to DNA polymerase and primers what else does a polymerase chain require?

Description : In addition to DNA polymerase and primers what else does a polymerase chain require?

Last Answer : Need answer

The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5’ end of the growing polynucleotide chain (C) DNA polymerases can add nucleotides at both ends of the chain (D) All RNA and DNA polymerases can add nucleotides only at the 3’ end of the growing polynucleotide chain

Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain

Last Answer : Answer : D

In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase

Description : In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase

Last Answer : Answer : B

An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix of DNA by an enzyme known as DNA polymerase b. The initial product of DNA transcription is called heterogeneous nuclear RNA which codes directly for proteins c. After processing is complete, the mRNA is exported from the nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete

Why is the halogenation of alkanes considered a chain reaction? (a) it occurs quickly. (b) it occurs without the generation of intermediates. (c) each step generates the reactive intermediate that causes the next step to occur. (d) the reaction allows long chains of halogenated alkanes to be formed

Description : Why is the halogenation of alkanes considered a chain reaction? (a) it occurs quickly. (b) it occurs without the generation of intermediates. (c) each step generates the reactive intermediate that causes the next step to occur. (d) the reaction allows long chains of halogenated alkanes to be formed

Last Answer : each step generates the reactive intermediate that causes the next step to occur

Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Description : Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Last Answer : Answer : A

Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Description : Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Last Answer : Answer : A

what side measures 50 feet. She also wants to fence in 3 square rose beds next to her garden. Each side of a bed measures 10.5 feet. How many feet of fencing does Laurel need in all?

Description : what side measures 50 feet. She also wants to fence in 3 square rose beds next to her garden. Each side of a bed measures 10.5 feet. How many feet of fencing does Laurel need in all?

Last Answer : 84.5

A 28 years old woman has 14 weeks size irregular uterus. She does not complain of abdominal pain or menorrhagia. Her pap smear is normal. The best next step in her management would be: a) Continued observation. b) Endometrial biopsy. c) Hysterectomy. d) Pelvic ultrasonography. e) Laparoscopy.

Description : A 28 years old woman has 14 weeks size irregular uterus. She does not complain of abdominal pain or menorrhagia. Her pap smear is normal. The best next step in her management would be: a) Continued observation. b) Endometrial biopsy. c) Hysterectomy. d) Pelvic ultrasonography. e) Laparoscopy.

Last Answer : continued observation is required in such a case as the patient is stable

What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

Description : What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

Last Answer : answer:

Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Description : Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Last Answer : Step 1 DNA isolation.Step 2 Primer design.Step 3 Enzyme selection.Step 4 Thermal cycling.Step 5 Amplicon analysis.

Why is polymerase chain reaction important? -Biology

Description : Why is polymerase chain reaction important? -Biology

Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.

Why is polymerase chain reaction important? -Biology

Description : Why is polymerase chain reaction important? -Biology

Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.

What does polymerase chain reaction (pcr) do?

Description : What does polymerase chain reaction (pcr) do?

Last Answer : Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.

The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Last Answer : Answer : D

Most commonly employed test for determining the antibody titers for Newcastle disease infection in B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Description : Most commonly employed test for determining the antibody titers for Newcastle disease infection in B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Last Answer : Hemagglutination inhibition

Most commonly employed test for determining the antibody titers for AI infection is B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Description : Most commonly employed test for determining the antibody titers for AI infection is B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Last Answer : Hemagglutination inhibition

Trichomoniasis can be diagnosed by D A. Whiff test B. Polymerase chain reaction C. Wet mount D. All above

Description : Trichomoniasis can be diagnosed by D A. Whiff test B. Polymerase chain reaction C. Wet mount D. All above

Last Answer : All above

The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

Last Answer : (d) denaturation, annealing, extension.

The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above

Description : The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above

Last Answer : all above