Description : Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)
Last Answer : (c)
Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends
Last Answer : Answer : D
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein
Last Answer : Answer : B
Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences
Last Answer : Answer : A
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Last Answer : Answer : C
Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA
Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false
Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.
Last Answer : d) Sticky ends can be joined by using DNA ligases.
Description : What is the significance of recognition sequences of restriction enzymes? -Biology
Last Answer : answer:
Description : Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Description : Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Description : How does the restriction enzyme prevent the cutting of the endogenous DNA? -Biology
Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease
Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.
Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease
Last Answer : a) Topoisomease II
Description : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using
Last Answer : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant ... B. Ligase C. Eco RI D. Taq polymerase
Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms
Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.
Last Answer : (a) they can cut DNA at specific base sequence
Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate
Last Answer : (a) E.coli restriction endonuclease I
Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.
Last Answer : (d) ligase.
Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope
Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro
Description : he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases
Last Answer : (b) Restriction endonucleases
Description : $ The regular sequences of a cell cycle is `G_(1),S,G_(2),M` and C ! Replication of DNA occurs during S phase.
Last Answer : $ The regular sequences of a cell cycle is `G_(1),S,G_(2),M` and C ! Replication of DNA occurs during ... , R is wrong D. If both As and R are wrong.
Description : What is complementary mRNA sequences for DNA bases A-T-C-A-G-G?
Last Answer : What is the answer ?
Description : The genetic code operates via (A) The protein moiety of DNA (B) The base sequences of DNA (C) The nucleotide sequence of mRNA (D) The base sequence of tRNA
Description : DNA finger printing is based on the presence in DNA of (A) Constant number of tandem repeats (B) Varibale number of tandem repeats (C) Non-repititive sequences in each DNA (D) Introns in eukaryotic DNA
Description : Yeast artificial chromosome can be used to amplify DNA sequences of the size (A) Upto 10 kb (B) Upto 45 kb (C) Upto 100 kb (D) Upto 1,000 kb
Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases
Description : Besides structural genes that encode proteins, DNA contains some regulatory sequences which are known as (A) Operons (B) Cistrons (C) Cis-acting elements (D) Trans-acting factors
Description : Satellite DNA contains (A) Highly repetitive sequences (B) Moderately repetitive sequences (C) Non-repetitive sequences (D) DNA-RNA hybrids
Description : The process of producing a protein from its DNA- and mRNA-coding sequences.
Last Answer : Ans. Gene expression
Description : Proto-oncogenes are: B A. DNA sequences in cells that are oncogenes themselves B. Cellular copies of genes that were first found in oncogenic retroviruses C. DNA viral sequences that are known to infect human cells D. Bacterial DNA counterparts
Last Answer : Cellular copies of genes that were first found in oncogenic retroviruses
Description : .Expressed Sequence Tags (ESTs) refers to (a) novel DNA sequences (b) genes expressed as RNA (c) polypeptide expression (d) DNA polymorphism.
Last Answer : (b) genes expressed as RNA
Description : In the regulation of genes: a. more than 90% of the base sequences in human DNA have not known function b. extrons are the part of the gene that code for amino acids found in the final proteins. c. introns usually begins with the nucleotide sequence GT d. all above
Last Answer : all above
Description : The eukaryotic genome differs from the prokaryotic genome because (a) the DNA is complexed with histone in prokaryotes (b) the DNA is circular and single stranded in prokaryotes (c) repetitive sequences are present in eukaryotes (d) genes in the former case are organised into operons.
Last Answer : (b) the DNA is circular and single stranded in prokaryotes
Description : Spontaneous mutations could arise from a. DNA replication errors. b. Atmospheric Radiation. c. Addition Of Insertion Sequences. d. All the above (A—C) are correct.
Last Answer : d. All the above (A—C) are correct.
Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase
Last Answer : d. Okazaki fragments; DNA ligase
Description : The role of molecular chaperones is to A-facilitate binding of ribosomes to mRNA B-degrade newly synthesized polypeptides that contain inaccurate sequences C-.facilitate binding of RNA polymerase to DNA D-aid a newly synthesized polypeptide in folding to its proper shape
Last Answer : -aid a newly synthesized polypeptide in folding to its proper shape
Last Answer : aid a newly synthesized polypeptide in folding to its proper shape
Description : What is the term used for a segment of DNA with one or more genes in the centre and the twoends carrying inverte d repeat sequences of nucleotides? A- Plasmid B- Transposon C- Insertion sequence D- None of these
Last Answer : Transposon
Description : The type of recombination that commonly occurs between a pair of homologous DNA sequences is, A- mutagenic recombination B- site-specific recombination C- replicative recombination D- general recombination
Last Answer : general recombination
Description : What information can be generated by interrupted mating experiments? A- Levels of DNA homology B- Bacterial genome maps C- DNA nucleotide sequences D- Proteomics of the bacteria
Last Answer : Bacterial genome maps
Description : Cistron is a) The coding sequence of DNA b) The functional unit of DNA molecule that codes for a particular gene product c) Intervening non coding sequence of DNA d) The sequences which are removed during RNA splicing.
Last Answer : b) The functional unit of DNA molecule that codes for a particular gene product c) Intervening non coding sequence of DNA
Description : How can I make this restriction enzyme digest better?
Last Answer : I haven't the foggiest idea what you speak of there, but I do know that staring at a problem too long alters your vision of the problem as a whole. Start your down time by completely disassociating yourself ... with your focus. Cleanse it, and come in fresh. That is the very best I can offer you.
Description : Who discovered the first restriction enzyme? -Biology
Description : The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)? Bam H1 Sal 1 Pvu II rop ... reduced osmotic pressure (c) HindIII, EcoRI - selectable markers (d) ampR, tetR-antibiotic resistance genes
Last Answer : (d) ampR, tetR–antibiotic resistance genes
Description : A selectable marker is used to (a) help in eliminating the non-transformants, so that the transformants can be regenerated (b) identify the gene for a desired trait in an alien organism (c) ... in a specific crop (d) mark a gene on a chromosome for isolation using restriction enzyme.
Last Answer : (a) help in eliminating the non-transformants, so that the transformants can be regenerated
Description : How are restriction enzymes used to make recombinant DNA? -Biology
Description : Role of restriction enzymes and DNA ligase -Biology