Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) The enzyme cuts DNA molecule at identified position within the DNA. (c) The enzyme binds DNA at specific sites and cuts only one of the two strands. (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Following statements describe the characteristics of
the enzyme restriction endonuclease. Identify the
incorrect statement.
(a) The enzyme recognises a specific palindromic
nucleotide sequence in the DNA.
(b) The enzyme cuts DNA molecule at identified
position within the DNA.
(c) The enzyme binds DNA at specific sites and
cuts only one of the two strands.
(d) The enzyme cuts the sugar-phosphate backbone
at specific sites on each strand.
by

1 Answer

Answer :

(c) The enzyme binds DNA at specific sites and
cuts only one of the two strands.
by

Related questions

Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Last Answer : d) Sticky ends can be joined by using DNA ligases.

Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Last Answer : (a) make cuts at specific positions within the DNA molecule

Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.

Description : Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.

Last Answer : (c) cuts the DNA molecule at specific sites

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5′ _____ GAATTC _____ 3′ 3′ _____ CTTAAG _____ 5′ (a) Replication completed (b) Deletion mutation (c) Start codon at the 5′ end (d) Palindromic sequence of base pairs

Description : Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5′ _____ GAATTC _____ 3′ 3′ _____ CTTAAG _____ 5′ (a) ... completed (b) Deletion mutation (c) Start codon at the 5′ end (d) Palindromic sequence of base pairs

Last Answer : (d) Palindromic sequence of base pairs

Antiparallel strands of a DNA molecule means that (a) one strand turns clockwise (b) one strand turns anti-clockwise (c) the phosphate groups of two DNA strands, at their ends, share the same position (d) the phosphate groups at the start of two DNA strands are in opposite position (pole).

Description : Antiparallel strands of a DNA molecule means that (a) one strand turns clockwise (b) one strand turns anti-clockwise (c) the phosphate groups of two DNA strands, at their ends, share the same position (d) the phosphate groups at the start of two DNA strands are in opposite position (pole).

Last Answer : (d) the phosphate groups at the start of two DNA strands are in opposite position (pole).

Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Last Answer : a) Topoisomease II

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequence of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequence of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Last Answer : Answer : C

DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Last Answer : Answer : C

Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate

Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate

Last Answer : (a) E.coli restriction endonuclease I

Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Description : Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Last Answer : Answer : A

Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Description : Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Last Answer : Answer : A

Choose the correct pair from the following. (a) Ligases - Join the two DNA molecules (b) Polymerases - Break the DNA into fragments (c) Nucleases - Separate the two strands of DNA (d) Exonucleases - Make cuts at specific positions within DNA

Description : Choose the correct pair from the following. (a) Ligases - Join the two DNA molecules (b) Polymerases - Break the DNA into fragments (c) Nucleases - Separate the two strands of DNA (d) Exonucleases - Make cuts at specific positions within DNA

Last Answer : (a) Ligases - Join the two DNA molecules

Chimeric DNA (A) Is found in bacteriophages (B) Contains unrelated genes (C) Has no restriction sites (D) Is palindromic

Description : Chimeric DNA (A) Is found in bacteriophages (B) Contains unrelated genes (C) Has no restriction sites (D) Is palindromic

Last Answer : Answer : B

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)

Description : Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)

Last Answer : (c)

Which one of the following statements is not true of RNA? a. RNA contains the monosaccharide ribose. b. RNA is primarily a single-stranded molecule. c. RNA has a sugar-phosphate backbone. d. RNA contains five different nucleotides.

Description : Which one of the following statements is not true of RNA? a. RNA contains the monosaccharide ribose. b. RNA is primarily a single-stranded molecule. c. RNA has a sugar-phosphate backbone. d. RNA contains five different nucleotides.

Last Answer : d. RNA contains five different nucleotides.

The structure of DNA is a double helix formed by two strands of DNA sequence. This sequence consists of 4 different nucleotides- Adenine, Thymine, Cytosine and Guanine. How do the nucleotides on one strand interact with the nucleotides on the second strand to maintain the helical shape of DNA?

Description : The structure of DNA is a double helix formed by two strands of DNA sequence. This sequence consists of 4 different nucleotides- Adenine, Thymine, Cytosine and Guanine. How do the nucleotides on one strand interact with the nucleotides on the second strand to maintain the helical shape of DNA?

Last Answer : Complementary Base Pairing. Adenine complementary base pairs with Thymine and Guanine complementary base pairs with Cytosine.

Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease

Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease

Last Answer : Answer : D

What determines where a restriction endonuclease cuts? -Biology

Description : What determines where a restriction endonuclease cuts? -Biology

Last Answer : answer:

During transcription, if the nucleotide sequence of the DNA strand that is being coded is ATACG then the nucleotide sequence in the mRNA would be (a) TATGC (b) TCTGG (c) UAUGC (d) UATGC.

Description : During transcription, if the nucleotide sequence of the DNA strand that is being coded is ATACG then the nucleotide sequence in the mRNA would be (a) TATGC (b) TCTGG (c) UAUGC (d) UATGC.

Last Answer : (c) UAUGC

During transcription, if the nucleotide sequence of the DNA strand that is being coded is ATACG then the nucleotide sequence in the mRNA would be (a) TATGC (b) TCTGG (c) UAUGC (d) UATGC.

Description : During transcription, if the nucleotide sequence of the DNA strand that is being coded is ATACG then the nucleotide sequence in the mRNA would be (a) TATGC (b) TCTGG (c) UAUGC (d) UATGC.

Last Answer : (c) UAUGC

All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Last Answer : Answer : D

Nucleotides are building blocks of nucleic acids. Each nucleotide is a composite molecule formed by (a) base-sugar-phosphate (b) base-sugar-OH (c) (base-sugar-phosphate)n (d) sugar-phosphate.

Description : Nucleotides are building blocks of nucleic acids. Each nucleotide is a composite molecule formed by (a) base-sugar-phosphate (b) base-sugar-OH (c) (base-sugar-phosphate)n (d) sugar-phosphate.

Last Answer : (a) base-sugar-phosphate

The 3′ - 5′ phosphodiester linkages inside a polynucleotide chain serve to join (a) one DNA strand with the other DNA strand (b) one nucleoside with another nucleoside (c) one nucleotide with another nucleotide (d) one nitrogenous base with pentose sugar.

Description : The 3′ - 5′ phosphodiester linkages inside a polynucleotide chain serve to join (a) one DNA strand with the other DNA strand (b) one nucleoside with another nucleoside (c) one nucleotide with another nucleotide (d) one nitrogenous base with pentose sugar.

Last Answer : (c) one nucleotide with another nucleotide

Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false

What is palindromic nucleotide sequence? -Biology

Description : What is palindromic nucleotide sequence? -Biology

Last Answer : answer:

The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Last Answer : (a) they can cut DNA at specific base sequence

Enzyme that cuts DNA at specific sites is

Description : Enzyme that cuts DNA at specific sites is

Last Answer : Enzyme that cuts DNA at specific sites is A. Restroction endonuclease B. DNA ligase C. DNA polymerase D. Reverse transcriptase

In the semiconservative method of replication a. Both parent strands are degraded. b. One parent strand is conserved in each of the the new dna. c. Both parent strands reform with one another. d. Okazaki fragments form both of the new molecules of DNA.

Description : In the semiconservative method of replication a. Both parent strands are degraded. b. One parent strand is conserved in each of the the new dna. c. Both parent strands reform with one another. d. Okazaki fragments form both of the new molecules of DNA.

Last Answer : b. One parent strand is conserved in each of the the new dna.

Which complementary sequence would NOT be recognized by a restriction endonucLease?a. GAATTC CTTAAG b. AAGCTT TTCGAA c. GTCGAC CAGCTG d. AATTCCTTAAGG

Description : Which complementary sequence would NOT be recognized by a restriction endonucLease? a. GAATTC CTTAAG b. AAGCTT TTCGAA c. GTCGAC CAGCTG d. AATTCCTTAAGG

Last Answer : d. AATTCCTTAAGG

Read the statements given below and identify the incorrect statement. a) The human genome contains 3164.7 million nucleotide bases. b) The average gene consists of 30,000 bp c) The total number of genes is estimated at 30,000. d) Chromosome Y has 231 genes e) Less than 2% of the genome codes for proteins.

Description : Read the statements given below and identify the incorrect statement. a) The human genome contains 3164.7 million nucleotide bases. b) The average gene consists of 30,000 bp c) The total number of genes is ... . d) Chromosome Y has 231 genes e) Less than 2% of the genome codes for proteins.

Last Answer : d) Chromosome Y has 231 genes

Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Last Answer : Answer : A

Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.

Description : Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.

Last Answer : (b) restriction endonuclease

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Last Answer : (d) ligase.

Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase

Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase

Last Answer : (a) restriction endonuclease

A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Last Answer : b. restriction endonuclease

Termination of the synthesis of the RNA molecule is signaled by a sequence in the template strand of the DNA molecule, a signal that is recognized by a termination protein, the (A) Rho (ρ) factor (B) σ factor (C) δ factor (D) ε factor

Description : Termination of the synthesis of the RNA molecule is signaled by a sequence in the template strand of the DNA molecule, a signal that is recognized by a termination protein, the (A) Rho (ρ) factor (B) σ factor (C) δ factor (D) ε factor

Last Answer : Answer : A

The unwound strands of DNA are held apart by (A) Single strand binding protein (B) Double strand binding protein (C) Rep protein (D) DNAA protein

Description : The unwound strands of DNA are held apart by (A) Single strand binding protein (B) Double strand binding protein (C) Rep protein (D) DNAA protein

Last Answer : Answer : A

Alkylating agents exert cytotoxic action by inducing: A. Breakage of DNA strand B. Cross linking of DNA strands C. Abnormal pairing of purine and pyrimidine bases D. All of the above

Description : Alkylating agents exert cytotoxic action by inducing: A. Breakage of DNA strand B. Cross linking of DNA strands C. Abnormal pairing of purine and pyrimidine bases D. All of the above

Last Answer : D. All of the above

mRNA is complementary to the nucleotide sequence of (A) Coding strand (B) Ribosomal RNA (C) tRNA (D) Template strand

Description : mRNA is complementary to the nucleotide sequence of (A) Coding strand (B) Ribosomal RNA (C) tRNA (D) Template strand

Last Answer : Answer : A

Which one of the following statements concerning glucose-6-phosphate dehydrogenase deficiency is correct? (A) Young R.B.Cs, particularly reticulocytes, contain the highest enzyme activity cells show less enzyme activity (B) Glucose-6-P Dehydroglucose deficiency leads to disfuction of many tissues (C) G-6-p Dehydroglucose deficiency is due to a single deletion of a large sequence of DNA in the G-6-PD gene (D) G-6-PD deficiency is precipitated by ingestion of drugs such as aspirin

Description : Which one of the following statements concerning glucose-6-phosphate dehydrogenase deficiency is correct? (A) Young R.B.Cs, particularly reticulocytes, contain the highest enzyme activity cells show less enzyme ... PD gene (D) G-6-PD deficiency is precipitated by ingestion of drugs such as aspirin

Last Answer : Answer : A

Which of the following statement(s) is/are true concerning DNA? a. DNA is contained only in the nucleus of the cell b. DNA strands are encoded by the sequence of four bases—adenine, guanine, cytosine and uridine c. The basic unit of information of DNA is the intron, a sequence of three bases d. There are an infinite number of possible codons

Description : Which of the following statement(s) is/are true concerning DNA? a. DNA is contained only in the nucleus of the cell b. DNA strands are encoded by the sequence of four bases-adenine, guanine, cytosine ... DNA is the intron, a sequence of three bases d. There are an infinite number of possible codons

Last Answer : Answer: a The genetic blueprint of an organism is carried in the nucleus of every cell, encoded by the sequence of four bases-adenine, guanine, cytosine and thymine, which together make up ... codons. Sixty-one of these code for amino acids and three are termination signals called stop codons

Give reason: The two strands of DNA molecule are complementary to each other but are not identical.

Description : Give reason: The two strands of DNA molecule are complementary to each other but are not identical.

Last Answer : The two strands of DNA are not identical because the nitrogenous bases are different in pairing. A purine base of one polynucleotide chain always pairs with a pyramidine base on the other. Adenine (A) pairs ... and Cytosine (C) pairs with Thymine (T). Hence the two strands of DNA are complementary.

Which of the statements give below is correct with respect to frameshift mutation a) a single nucleotide base change, insertion, or deletion of the genetic material b) Glutamine is replaced by valine c) Sickle cell anemia is an example d) insertions or deletions of a number of nucleotides in a DNA sequence that is not divisible by three.

Description : Which of the statements give below is correct with respect to frameshift mutation a) a single nucleotide base change, insertion, or deletion of the genetic material b) Glutamine is replaced by valine c) ... or deletions of a number of nucleotides in a DNA sequence that is not divisible by three.

Last Answer : d) insertions or deletions of a number of nucleotides in a DNA sequence that is not divisible by three.

A selectable marker is used to (a) help in eliminating the non-transformants, so that the transformants can be regenerated (b) identify the gene for a desired trait in an alien organism (c) select a suitable vector for transformation in a specific crop (d) mark a gene on a chromosome for isolation using restriction enzyme.

Description : A selectable marker is used to (a) help in eliminating the non-transformants, so that the transformants can be regenerated (b) identify the gene for a desired trait in an alien organism (c) ... in a specific crop (d) mark a gene on a chromosome for isolation using restriction enzyme.

Last Answer : (a) help in eliminating the non-transformants, so that the transformants can be regenerated

An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix of DNA by an enzyme known as DNA polymerase b. The initial product of DNA transcription is called heterogeneous nuclear RNA which codes directly for proteins c. After processing is complete, the mRNA is exported from the nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete

Which one of the following represents a palindromic sequence in DNA? (a) 5′ - GAATTC - 3′ (b) 5′ - CCAATG - 3′ 3′ - CTTAAG - 5′ 3′ - GAATCC - 5′ (c) 5′ - CATTAG - 3′ (d) 5′ - GATACC - 3′ 3′ - GATAAC - 5′ 3′ - CCTAAG - 5′

Description : Which one of the following represents a palindromic sequence in DNA? (a) 5′ - GAATTC - 3′ (b) 5′ - CCAATG - 3′ 3′ - CTTAAG - 5′ 3′ - GAATCC - 5′ (c) 5′ - CATTAG - 3′ (d) 5′ - GATACC - 3′ 3′ - GATAAC - 5′ 3′ - CCTAAG - 5′

Last Answer : (a) 5′ - GAATTC - 3′ 5′ - CCAATG - 3′ 3′ - CTTAAG - 5′