Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.
Description : Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.
Last Answer : (b) restriction endonuclease
Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase
Last Answer : (a) restriction endonuclease
Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNA polymerase
Last Answer : (3) Helicase Explanation: A type of helicase enzyme called DNA helicase catalyzes the unwinding of the DNA helix at the time of replication. It uses the energy released through hydrolysis of ATP ( ... The enzyme is needed to separate the two strands of DNA also during transcription and DNA repair.
Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNApolymerase
Last Answer : C option is the answer
Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase
Last Answer : d. RNA polymerase
Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease
Last Answer : Answer : D
Description : Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease
Last Answer : a) Ligase
Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA
Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false
Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.
Last Answer : d) Sticky ends can be joined by using DNA ligases.
Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping
Last Answer : Answer : C
Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate
Last Answer : (a) E.coli restriction endonuclease I
Description : Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.
Last Answer : (c) cuts the DNA molecule at specific sites
Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.
Last Answer : (d) ligase.
Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase
Last Answer : b. restriction endonuclease
Description : Which of the following is a restriction endonuclease? (a) DNase I (b) RNase (c) Hind II (d) Protease
Last Answer : (c) Hind II
Description : Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase
Last Answer : b) DNA helicase
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Description : What is the role of the enzyme DNA primase?
Last Answer : Primase is an enzyme that synthesizes short RNA sequences called primers which serve as a starting point for DNA synthesis. Since primase produces RNA molecules, the enzyme is a type of RNA ... strand of nucleotides. Therefore, primase serves to prime and lay a foundation for DNA synthesis.
Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III
Last Answer : Answer : B
Description : The removal of which enzyme affects the synthesis of hnRNA in eukaryotes a) RNA polymerase II b) RNA primase c) RNA polymerase III d) RNA polymerase I
Last Answer : a) RNA polymerase II
Description : What determines where a restriction endonuclease cuts? -Biology
Last Answer : answer:
Description : Function of restriction endonuclease in bacteria -Biology
Description : What is restriction endonuclease used for? -Biology
Description : There is a restriction endonuclease called EcoRI. What does “co” part in it stand for? (a) colon (b) coelom (c) coenzyme (d) coli
Last Answer : (d) coli
Description : Which complementary sequence would NOT be recognized by a restriction endonucLease? a. GAATTC CTTAAG b. AAGCTT TTCGAA c. GTCGAC CAGCTG d. AATTCCTTAAGG
Last Answer : d. AATTCCTTAAGG
Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Description : Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ
Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand
Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete
Description : During the process of isolation of DNA, chilled ethanol is added to (a) precipitate DNA (b) break open the cell to release DNA (c) facilitate action of restriction enzymes (d) remove proteins such as histones.
Last Answer : a) precipitate DNA
Description : How does the restriction enzyme prevent the cutting of the endogenous DNA? -Biology
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)
Last Answer : (c)
Description : 6. Which of the following DNA sequences would a Restriction enzyme recognize and cut? a. ATGCAC TACGTG b. GATATC CTATAG c. TAGATA ATCTAT d. AATATA TTATAT
Last Answer : b. GATATC CTATAG
Description : From a DNA-RNA hybrid, DNA can be obtained by addition of (A) DNA B protein and ATP (B) Helicase and ATP (C) DNA topoisomerase I (D) Alkali
Description : Negative supercoils are introduced in DNA by (A) Helicase (B) DNA ligase (C) DNA gyrase (D) DNA polymerase III holoenzyme
Description : Helicase and DNAB protein cause (A) Rewinding of DNA and require ATP as a source of energy (B) Rewinding of DNA but do not require any source of energy (C) Unwinding of DNA and require ATP as a source of energy (D) Unwinding of DNA but do not require any source of energy
Description : An eazyme produced by HIV that allows the integration of HIV DNA into the host cell's DNA is (1) Ligase (2) Integrase (3) Helicase (4) DNA gyrase
Last Answer : (2) Integrase Explanation: HIV's enzyme for inserting the DNA version of its genome into the host cell DNA is called its "integrase". HIV-1 integrase catalyzes the "cut-and-paste" action of ... to the clipped ends. It is also produced by viruses containing double-stranded DNAs for the same purpose.
Description : During DNA replication, the strands separate by (a) DNA polymerase (b) topoisomerase (c) unwindase/helicase (d) gyrase.
Last Answer : b) topoisomerase
Description : An eazyme produced by HIV that allows the integration of HIV DNA into the host cell’s DNA is : (1) Ligase (2) Integrase (3) Helicase (4) DNA gyrase
Last Answer : Integrase
Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase
Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease
Description : The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)? Bam H1 Sal 1 Pvu II rop ... reduced osmotic pressure (c) HindIII, EcoRI - selectable markers (d) ampR, tetR-antibiotic resistance genes
Last Answer : (d) ampR, tetR–antibiotic resistance genes
Description : 1. Bacteria protect themselves from viruses by fragmenting viral DNA with a. Ligase b. Endonuclease c. Exonuclease d. Gyrase
Last Answer : Ans: Endonuclease.
Description : What enzyme unwind or unzips the parent strand?
Last Answer : Helicase.
Description : How can I make this restriction enzyme digest better?
Last Answer : I haven't the foggiest idea what you speak of there, but I do know that staring at a problem too long alters your vision of the problem as a whole. Start your down time by completely disassociating yourself ... with your focus. Cleanse it, and come in fresh. That is the very best I can offer you.