In addition to DNA polymerase and primers what else does a polymerase chain require?

In addition to DNA polymerase and primers what else does a polymerase chain require?
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In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Last Answer : Answer : D

The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5’ end of the growing polynucleotide chain (C) DNA polymerases can add nucleotides at both ends of the chain (D) All RNA and DNA polymerases can add nucleotides only at the 3’ end of the growing polynucleotide chain

Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain

Last Answer : Answer : D

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 5 3 5 3 5 3 5 3 5 5 3 3 5 3 5 3 Region to be amplified . (a) B - denaturation at a temperature of about 98°C separating the two DNA strands (b) A - denaturation at a temperature of about 50°C (c) C - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Description : The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 ... - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Last Answer : (c) C - extension in the presence of heat stable DNA polymerase

DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication

Description : DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication

Last Answer : Answer : C

The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers

Description : The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers

Last Answer : Answer : B

Which of the following statement is false (a). Enzymes are differentiated by electrophoresis method (b). While DNA sequencing both forward and reverse primers are used (c). Amplification is done through PCR (d). ABO blood grouping is mainly used for differentiating individuals

Description : Which of the following statement is false (a). Enzymes are differentiated by electrophoresis method (b). While DNA sequencing both forward and reverse primers are used (c). Amplification is done through PCR (d). ABO blood grouping is mainly used for differentiating individuals

Last Answer : Ans: (b). While DNA sequencing both forward and reverse primers are used 

A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Last Answer : use restriction enzymes is the right answer for apex :)

A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Last Answer : use restriction enzymes is the right answer for apex :)

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Last Answer : Answer : C

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Last Answer : Answer : C

The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

Description : The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

Last Answer : Answer : C

Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Last Answer : Answer : A

The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for both antigen and antibody detection b. Southern, northern, and western immunoblot techniques are used to detect DNA, RNA, or proteins, respectively c. Polymerase chain reaction (PCR) is a sensitive assay used to detect small amounts of microbial DNA, thus detecting infection at its early stages d. Infectious agents currently detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Description : DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Last Answer : (a) electrophoresis

Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis

Description : Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis

Last Answer : (d) Zinc finger analysis

Do you remember the names of the characters in the primers/readers when you first learned to read?

Description : Do you remember the names of the characters in the primers/readers when you first learned to read?

Last Answer : I think it was Dick and Jane but I’m not positive. This was in the early 70’s.

What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

Description : What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

Last Answer : answer:

Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Description : Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Last Answer : Step 1 DNA isolation.Step 2 Primer design.Step 3 Enzyme selection.Step 4 Thermal cycling.Step 5 Amplicon analysis.

Why is polymerase chain reaction important? -Biology

Description : Why is polymerase chain reaction important? -Biology

Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.

Why is polymerase chain reaction important? -Biology

Description : Why is polymerase chain reaction important? -Biology

Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.

What does polymerase chain reaction (pcr) do?

Description : What does polymerase chain reaction (pcr) do?

Last Answer : Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.

The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Last Answer : Answer : D

Most commonly employed test for determining the antibody titers for Newcastle disease infection in B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Description : Most commonly employed test for determining the antibody titers for Newcastle disease infection in B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Last Answer : Hemagglutination inhibition

Most commonly employed test for determining the antibody titers for AI infection is B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Description : Most commonly employed test for determining the antibody titers for AI infection is B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Last Answer : Hemagglutination inhibition

Trichomoniasis can be diagnosed by D A. Whiff test B. Polymerase chain reaction C. Wet mount D. All above

Description : Trichomoniasis can be diagnosed by D A. Whiff test B. Polymerase chain reaction C. Wet mount D. All above

Last Answer : All above

The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

Last Answer : (d) denaturation, annealing, extension.

The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above

Description : The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above

Last Answer : all above

Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction? A- HIV-1 and HIV-2 viruses B- Hepatitis B virus C- Mycobacterium tuberculosis D- All of these

Description : Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction? A- HIV-1 and HIV-2 viruses B- Hepatitis B virus C- Mycobacterium tuberculosis D- All of these

Last Answer : All of these

An important method used in the rapid identification of a pathogen is a. RNA gene sequencing b. polymerase chain reaction c. molecular taxonomy d. biochemical tests

Description : An important method used in the rapid identification of a pathogen is a. RNA gene sequencing b. polymerase chain reaction c. molecular taxonomy d. biochemical tests

Last Answer : d. biochemical tests

The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension

Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension

Last Answer : (4) Denaturation, Annealing, Extension

What is the difference between DNA and RNA polymerase?

Description : What is the difference between DNA and RNA polymerase?

Last Answer : DNA polymerase is responsible for synthesizing new strands of DNA during DNA replication, while RNA polymerase is responsible for synthesizing mRNA during transcription.

What is exonuclease activity of DNA polymerase? -Biology

Description : What is exonuclease activity of DNA polymerase? -Biology

Last Answer : answer:

What is the role of DNA polymerase in DNA replication? -Biology

Description : What is the role of DNA polymerase in DNA replication? -Biology

Last Answer : answer:

What is the function of DNA polymerase? -Biology

Description : What is the function of DNA polymerase? -Biology

Last Answer : answer:

What is the function of DNA polymerase III? -Biology

Description : What is the function of DNA polymerase III? -Biology

Last Answer : answer:

Function of DNA polymerase during DNA replication. -Biology

Description : Function of DNA polymerase during DNA replication. -Biology

Last Answer : answer:

When is DNA polymerase used? -Biology

Description : When is DNA polymerase used? -Biology

Last Answer : answer:

When is DNA polymerase used? -Biology

Description : When is DNA polymerase used? -Biology

Last Answer : answer:

How does DNA polymerase prevent mutation?

Description : How does DNA polymerase prevent mutation?

Last Answer : It checks DNA for errors during replication.

DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the

Description : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the

Last Answer : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the ... Antistrand C. Template strand D. Coding strand

When does. DNA polymerase check for mutations?

Description : When does. DNA polymerase check for mutations?

Last Answer : During DNA replication

The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin

Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin

Last Answer : Answer : B

Another name for reverse transcriptase is (A) DNA dependent DNA polymerase (B) DNA dependent RNA polymerase (C) RNA dependent DNA polymerase (D) RNA dependent RNA polymerase

Description : Another name for reverse transcriptase is (A) DNA dependent DNA polymerase (B) DNA dependent RNA polymerase (C) RNA dependent DNA polymerase (D) RNA dependent RNA polymerase

Last Answer : Answer : C

RNA directed DNA polymerase is (A) Replicase (B) Transcriptase (C) Reversetranscriptase (D) Polymerase–III

Description : RNA directed DNA polymerase is (A) Replicase (B) Transcriptase (C) Reversetranscriptase (D) Polymerase–III

Last Answer : Answer : C

DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III

Description : DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III

Last Answer : Answer : B

The antibiotic which inhibits DNA dependent RNA polymerase is (A) Mitomycin C (B) Actinomycin d (C) Streptomycin (D) Puromycin

Description : The antibiotic which inhibits DNA dependent RNA polymerase is (A) Mitomycin C (B) Actinomycin d (C) Streptomycin (D) Puromycin

Last Answer : Answer : B

Optimum temperature of DNA polymerase of T. aquaticus is (A) 30°C (B) 37°C (C) 54°C (D) 72°C

Description : Optimum temperature of DNA polymerase of T. aquaticus is (A) 30°C (B) 37°C (C) 54°C (D) 72°C

Last Answer : Answer : D

Myeloma cells and lymphocytes can be fused by using (A) Calcium chloride (B) Ethidium bromide (C) Polyethylene glycol (D) DNA polymerase

Description : Myeloma cells and lymphocytes can be fused by using (A) Calcium chloride (B) Ethidium bromide (C) Polyethylene glycol (D) DNA polymerase

Last Answer : Answer : C

Xeroderma pigmentosum results from a defect in (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these

Description : Xeroderma pigmentosum results from a defect in (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these

Last Answer : Answer : A

Damage to DNA caused by ultraviolet radiation can be repaired by (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these

Description : Damage to DNA caused by ultraviolet radiation can be repaired by (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these

Last Answer : Answer : D