Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
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Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Description : Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Last Answer : Answer : A

Select the incorrect statement out of the five given below about lac operon when Lactose is present in the medium. a) Gene – A gets transcribed into mRNA which produces β-galactoside permease b) Inducer-Repressor complex is formed c) Lactose inactivates repressor protein d) RNA polymerase transcribe Z-gene, Y-gene and A-gene e) Allolactose is the inducer of lac operon

Description : Select the incorrect statement out of the five given below about lac operon when Lactose is present in the medium. a) Gene - A gets transcribed into mRNA which produces β-galactoside permease b) ... polymerase transcribe Z-gene, Y-gene and A-gene e) Allolactose is the inducer of lac operon

Last Answer : Gene – A gets transcribed into mRNA which produces β-galactoside permease b) Inducer-Repressor complex is formed

All of the following statements about eukaryotic promoters are true except (A) They may be located upstream or down stream from the structural gene (B) They have two consensus sequences (C) One consensus sequence binds RNA polymerase (D) Mutations in promoter region can decrease the efficiency of transcription of the structural gene

Description : All of the following statements about eukaryotic promoters are true except (A) They may be located upstream or down stream from the structural gene (B) They have two consensus sequences ( ... (D) Mutations in promoter region can decrease the efficiency of transcription of the structural gene

Last Answer : Answer : A

.In negative operon, (a) co-repressor binds with repressor (b) co-repressor does not bind with repressor (c) co-repressor binds with inducer (d) cAMP have negative effect on lac operon. (

Description : .In negative operon, (a) co-repressor binds with repressor (b) co-repressor does not bind with repressor (c) co-repressor binds with inducer (d) cAMP have negative effect on lac operon. (

Last Answer : co-repressor binds with repressor

The function of a repressor protein in an operon system is to prevent synthesis by binding to (A) The ribosome (B) A specific region of the operon preventing transcription of structural genes (C) The RNA polymerase (D) A specific region of the mRNA preventing translation to protein

Description : The function of a repressor protein in an operon system is to prevent synthesis by binding to (A) The ribosome (B) A specific region of the operon preventing transcription of structural genes (C) The RNA polymerase (D) A specific region of the mRNA preventing translation to protein

Last Answer : Answer : B

Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) The enzyme cuts DNA molecule at identified position within the DNA. (c) The enzyme binds DNA at specific sites and cuts only one of the two strands. (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.

Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Last Answer : (a) make cuts at specific positions within the DNA molecule

DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Last Answer : Answer : C

Lactose or its analogues act as positive regulators of lac operon by (A) Attaching to i gene and preventing its expression (B) Increasing the synthesis of catabolite gene activator protein (C) Attaching to promoter region and facilitating the binding of RNA polymerase holoenzyme (D) Binding to repressor subunits so that the repressor cannot attach to the operator locus

Description : Lactose or its analogues act as positive regulators of lac operon by (A) Attaching to i gene and preventing its expression (B) Increasing the synthesis of catabolite gene activator protein (C) ... holoenzyme (D) Binding to repressor subunits so that the repressor cannot attach to the operator locus

Last Answer : Answer : D

RNA polymerase holoenzyme binds to lac operon at the following site: (A) i gene (B) z gene (C) Operator locus (D) Promoter region

Description : RNA polymerase holoenzyme binds to lac operon at the following site: (A) i gene (B) z gene (C) Operator locus (D) Promoter region

Last Answer : Answer : D

Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Last Answer : Answer : A

All of the following statements about bacterial promoters are true except (A) They are smaller than eukaryotic promoters (B) They have two consensus sequences upstream from the transcription star site (C) TATA box is the site for attachment of RNA polymerase (D) TATA box has a high melting temperature

Description : All of the following statements about bacterial promoters are true except (A) They are smaller than eukaryotic promoters (B) They have two consensus sequences upstream from the transcription star site (C) ... is the site for attachment of RNA polymerase (D) TATA box has a high melting temperature

Last Answer : Answer : D

In the ’lac operon’ concept, which of the following is a protein? (A) Operator (B) Repressor (C) Inducer (D) Vector

Description : In the ’lac operon’ concept, which of the following is a protein? (A) Operator (B) Repressor (C) Inducer (D) Vector

Last Answer : Answer : B

Positive control of induction is best described as a control system in which an operon functions (A) Unless it is switched off by a derepressed repressor protein (B) Only after a repressor protein is inactivated by an inducer (C) Only after an inducer protein, which can be inactivated by a corepressor, switches it on (D) Only after an inducer protein, which is activated by an inducer, switch it on

Description : Positive control of induction is best described as a control system in which an operon functions (A) Unless it is switched off by a derepressed repressor protein (B) Only after a repressor protein is ... on (D) Only after an inducer protein, which is activated by an inducer, switch it on

Last Answer : Answer : D

In the lac operon concept, a protein molecule is (A) Operator (B) Inducer (C) Promoter (D) Repressor

Description : In the lac operon concept, a protein molecule is (A) Operator (B) Inducer (C) Promoter (D) Repressor

Last Answer : Answer : D

Negative regulation of protein synthesis is accomplished by A- allosteric inhibition B- the binding of RNA polymerase to the promoter C- the binding of a repressor to the DNA D- the binding of a repressor to the RNA polymerase

Description : Negative regulation of protein synthesis is accomplished by A- allosteric inhibition B- the binding of RNA polymerase to the promoter C- the binding of a repressor to the DNA D- the binding of a repressor to the RNA polymerase

Last Answer : the binding of a repressor to the DNA

During transcription, the DNA site at which RNA polymerase binds is called (a) promoter (b) regulator (c) receptor (d) enhancer.

Description : During transcription, the DNA site at which RNA polymerase binds is called (a) promoter (b) regulator (c) receptor (d) enhancer.

Last Answer : d) enhancer

During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box

Description : During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box

Last Answer : (c) GGTT box

During transcription, the DNA site at which RNA polymerase binds is called (a) promoter (b) regulator (c) receptor (d) enhancer.

Description : During transcription, the DNA site at which RNA polymerase binds is called (a) promoter (b) regulator (c) receptor (d) enhancer.

Last Answer : (a) promoter

During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box

Description : During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box

Last Answer : (b) TATA box

At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase

Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase

Last Answer : d. Okazaki fragments; DNA ligase

The role of molecular chaperones is to A-facilitate binding of ribosomes to mRNA B-degrade newly synthesized polypeptides that contain inaccurate sequences C-.facilitate binding of RNA polymerase to DNA D-aid a newly synthesized polypeptide in folding to its proper shape

Description : The role of molecular chaperones is to A-facilitate binding of ribosomes to mRNA B-degrade newly synthesized polypeptides that contain inaccurate sequences C-.facilitate binding of RNA polymerase to DNA D-aid a newly synthesized polypeptide in folding to its proper shape

Last Answer : -aid a newly synthesized polypeptide in folding to its proper shape

The role of molecular chaperones is to A-facilitate binding of ribosomes to mRNA B-degrade newly synthesized polypeptides that contain inaccurate sequences C-.facilitate binding of RNA polymerase to DNA D-aid a newly synthesized polypeptide in folding to its proper shape

Description : The role of molecular chaperones is to A-facilitate binding of ribosomes to mRNA B-degrade newly synthesized polypeptides that contain inaccurate sequences C-.facilitate binding of RNA polymerase to DNA D-aid a newly synthesized polypeptide in folding to its proper shape

Last Answer : aid a newly synthesized polypeptide in folding to its proper shape

Repressor binds to DNA sequence and regulate the transcription. This sequence is called (A) Attenuator (B) Terminator (C) Anti terminator (D) Operator

Description : Repressor binds to DNA sequence and regulate the transcription. This sequence is called (A) Attenuator (B) Terminator (C) Anti terminator (D) Operator

Last Answer : Answer : D

A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase

Last Answer : b. restriction endonuclease

The genetic code operates via (A) The protein moiety of DNA (B) The base sequences of DNA (C) The nucleotide sequence of mRNA (D) The base sequence of tRNA

Description : The genetic code operates via (A) The protein moiety of DNA (B) The base sequences of DNA (C) The nucleotide sequence of mRNA (D) The base sequence of tRNA

Last Answer : Answer : C

Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Last Answer : Answer : D

In Lac-operon, repressor protein binds to

Description : In Lac-operon, repressor protein binds to

Last Answer : In Lac-operon, repressor protein binds to A. Regulator gene B. Operator gene C. Promoter gene D. Structural gene

Which one of the following is wrongly matched? (a) Transcription - Writing information from DNA to tRNA. (b) Translation - Using information in mRNA to make protein. (c) Repressor protein - Binds to operator to stop enzyme synthesis. (d) Operon - Structural genes, operator and promoter.

Description : Which one of the following is wrongly matched? (a) Transcription - Writing information from DNA to tRNA. (b) Translation - Using information in mRNA to make protein. (c) Repressor protein - Binds to operator to stop enzyme synthesis. (d) Operon - Structural genes, operator and promoter.

Last Answer : (c) Repressor protein - Binds to operator to stop enzyme synthesis.

In E. coli, during lactose metabolism repressor binds to (a) regulator gene (b) operator gene (c) structural gene (d) promoter gen

Description : In E. coli, during lactose metabolism repressor binds to (a) regulator gene (b) operator gene (c) structural gene (d) promoter gen

Last Answer : (b) operator gene

Select the two correct statements out of the four (i – iv) statements given below about lac operon. (i) Glucose or galactose may bind with the repressor and inactivate it. (ii) In the absence of lactose, the repressor binds with the operator region. (iii)The z-gene codes for permease. (iv) This was elucidated by Francois Jacob and Jacques Monod. The correct statements are (a) (ii) and (iii) (b) (i) and (iii) (c) (ii) and (iv) (d) (i) and (ii)

Description : Select the two correct statements out of the four (i - iv) statements given below about lac operon. (i) Glucose or galactose may bind with the repressor and inactivate it. (ii) In the absence of lactose, the repressor binds with the ... (iii) (b) (i) and (iii) (c) (ii) and (iv) (d) (i) and (ii)

Last Answer : (c) (ii) and (iv)

Which one of the following is wrongly matched? (a) Transcription - Writing information from DNA to tRNA. (b) Translation - Using information in mRNA to make protein. (c) Repressor protein - Binds to operator to stop enzyme synthesis. (d) Operon - Structural genes, operator and promoter.

Description : Which one of the following is wrongly matched? (a) Transcription - Writing information from DNA to tRNA. (b) Translation - Using information in mRNA to make protein. (c) Repressor protein - Binds to operator to stop enzyme synthesis. (d) Operon - Structural genes, operator and promoter.

Last Answer : (d) Operon - Structural genes, operator and promoter

All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Last Answer : Answer : D

Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Last Answer : Answer : A

The ‘rho’ (ρ) factor is involved (A) To increase the rate of RNA synthesis (B) In binding catabolite repressor to the promoter region (C) In proper termination of transcription (D) To allow proper initiation of transcriptide

Description : The ‘rho’ (ρ) factor is involved (A) To increase the rate of RNA synthesis (B) In binding catabolite repressor to the promoter region (C) In proper termination of transcription (D) To allow proper initiation of transcriptide

Last Answer : Answer : C

In the regulation of genes: a. more than 90% of the base sequences in human DNA have not known function b. extrons are the part of the gene that code for amino acids found in the final proteins. c. introns usually begins with the nucleotide sequence GT d. all above

Description : In the regulation of genes: a. more than 90% of the base sequences in human DNA have not known function b. extrons are the part of the gene that code for amino acids found in the final proteins. c. introns usually begins with the nucleotide sequence GT d. all above

Last Answer : all above

Which of the following nucleotide sequences contains 4 pyrimidine bases? (a) GATCAATGC (b) GCUAGACAA (c) UAGCGGUAA (d) Both (b) and (c)

Description : Which of the following nucleotide sequences contains 4 pyrimidine bases? (a) GATCAATGC (b) GCUAGACAA (c) UAGCGGUAA (d) Both (b) and (c)

Last Answer : a) GATCAATGC

What information can be generated by interrupted mating experiments? A- Levels of DNA homology B- Bacterial genome maps C- DNA nucleotide sequences D- Proteomics of the bacteria

Description : What information can be generated by interrupted mating experiments? A- Levels of DNA homology B- Bacterial genome maps C- DNA nucleotide sequences D- Proteomics of the bacteria

Last Answer : Bacterial genome maps

Evolutionary relationships between groups of organisms are determined using which of Thefollowing type of information? A- Comparisons of nucleotide sequences B- .Comparisons of biochemical pathways C- Comparisons of structural features D- All of the above

Description : Evolutionary relationships between groups of organisms are determined using which of Thefollowing type of information? A- Comparisons of nucleotide sequences B- .Comparisons of biochemical pathways C- Comparisons of structural features D- All of the above

Last Answer : All of the above

Actinomycin D binds to (A) Double stranded DNA (B) Single stranded DNA (C) Single stranded RNA (D) DNA-RNA hybrid

Description : Actinomycin D binds to (A) Double stranded DNA (B) Single stranded DNA (C) Single stranded RNA (D) DNA-RNA hybrid

Last Answer : Answer : A

Satellite DNA contains (A) Highly repetitive sequences (B) Moderately repetitive sequences (C) Non-repetitive sequences (D) DNA-RNA hybrids

Description : Satellite DNA contains (A) Highly repetitive sequences (B) Moderately repetitive sequences (C) Non-repetitive sequences (D) DNA-RNA hybrids

Last Answer : Answer : A

The mechanism of synthesis of DNA and RNA are similar to all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is (D) They require a primer

Description : The mechanism of synthesis of DNA and RNA are similar to all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is (D) They require a primer

Last Answer : Answer : D

The mechanism of synthesis of DNA and RNA are similar in all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is 5’ → 3’ (D) They require a primer

Description : The mechanism of synthesis of DNA and RNA are similar in all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is 5’ → 3’ (D) They require a primer

Last Answer : Answer : A

The genetic code operates through (A) The protein moiety of DNA (B) Cistrom of DNA (C) Nucleotide sequence of m RNA (D) The anticodons of tRNA

Description : The genetic code operates through (A) The protein moiety of DNA (B) Cistrom of DNA (C) Nucleotide sequence of m RNA (D) The anticodons of tRNA

Last Answer : Answer : C

mRNA is complementary to the nucleotide sequence of (A) Coding strand (B) Ribosomal RNA (C) tRNA (D) Template strand

Description : mRNA is complementary to the nucleotide sequence of (A) Coding strand (B) Ribosomal RNA (C) tRNA (D) Template strand

Last Answer : Answer : A

What is antisense technology? (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene (b) RNA polymerase producing DNA (c) A cell displaying a foreign antigen used for synthesis of antigens (d) Production of somaclonal variants in tissue cultures

Description : What is antisense technology? (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene (b) RNA polymerase producing DNA (c) A cell displaying a foreign antigen used for synthesis of antigens (d) Production of somaclonal variants in tissue cultures

Last Answer : (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

What is the significance of recognition sequences of restriction enzymes? -Biology

Description : What is the significance of recognition sequences of restriction enzymes? -Biology

Last Answer : answer:

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)

Description : Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)

Last Answer : (c)

6. Which of the following DNA sequences would a Restriction enzyme recognize and cut? a. ATGCAC TACGTG b. GATATC CTATAG c. TAGATA ATCTAT d. AATATA TTATAT

Description : 6. Which of the following DNA sequences would a Restriction enzyme recognize and cut? a. ATGCAC TACGTG b. GATATC CTATAG c. TAGATA ATCTAT d. AATATA TTATAT

Last Answer : b. GATATC CTATAG

Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction fragment length polymorphism (RFLP) is usually formed in coding sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide

Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide