Enzyme that cuts DNA at specific sites is

Enzyme that cuts DNA at specific sites is
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Enzyme that cuts DNA at specific sites is A. Restroction endonuclease B. DNA ligase C. DNA polymerase D. Reverse transcriptase
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Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) The enzyme cuts DNA molecule at identified position within the DNA. (c) The enzyme binds DNA at specific sites and cuts only one of the two strands. (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.

Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.

Description : Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.

Last Answer : (c) cuts the DNA molecule at specific sites

Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Last Answer : (a) make cuts at specific positions within the DNA molecule

Choose the correct pair from the following. (a) Ligases - Join the two DNA molecules (b) Polymerases - Break the DNA into fragments (c) Nucleases - Separate the two strands of DNA (d) Exonucleases - Make cuts at specific positions within DNA

Description : Choose the correct pair from the following. (a) Ligases - Join the two DNA molecules (b) Polymerases - Break the DNA into fragments (c) Nucleases - Separate the two strands of DNA (d) Exonucleases - Make cuts at specific positions within DNA

Last Answer : (a) Ligases - Join the two DNA molecules

Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Last Answer : d) Sticky ends can be joined by using DNA ligases.

The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Last Answer : (a) they can cut DNA at specific base sequence

Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro

Select the total number of false statement from the following (i) The globular head is an active ATPase enzyme and has binding sites for ATP and activ

Description : Select the total number of false statement from the following (i) The globular head is an active ATPase enzyme and has binding sites for ATP and activ

Last Answer : Select the total number of false statement from the following (i) The globular head is an active ATPase enzyme and has ... arm A. 5 B. 4 C. 2 D. 1

If a coenzyme is required in an enzyme reaction, the former usually has the function of (A) Acting as an acceptor for one of the cleavage products of the substrate (B) Enhancing the specificity of the apo enzyme (C) Increasing the number of receptor sites of the apo enzyme (D) Activating the substrate ENZYMES 167

Description : If a coenzyme is required in an enzyme reaction, the former usually has the function of (A) Acting as an acceptor for one of the cleavage products of the substrate (B) Enhancing the specificity ... C) Increasing the number of receptor sites of the apo enzyme (D) Activating the substrate ENZYMES 167

Last Answer : Answer : A

Leguminous plants are able to fix atmospheric nitrogen through the process of symbiotic nitrogen fixation. Which one of the following statements is not correct during this process of nitrogen fixation? (a) Leghaemoglobin scavenges oxygen and is pinkish in colour. (b) Nodules act as sites for nitrogen fixation. (c) The enzyme nitrogenase catalyses the conversion of atmospheric N2 to NH3. (d) Nitrogenase is insensitive to oxygen.

Description : Leguminous plants are able to fix atmospheric nitrogen through the process of symbiotic nitrogen fixation. Which one of the following statements is not correct during this process of nitrogen fixation ... the conversion of atmospheric N2 to NH3. (d) Nitrogenase is insensitive to oxygen.

Last Answer : (d) Nitrogenase is insensitive to oxygen.

All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non-coding regions of DNA (C) It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease

Description : All the following statements about restriction fragment length polymorphism are true except (A) It results from mutations in restriction sites (B) Mutations in restriction sites can occur in coding or non- ... It is inherited in Mendelian fashion (D) It can be used to diagnose any genetic disease

Last Answer : Answer : D

Chimeric DNA (A) Is found in bacteriophages (B) Contains unrelated genes (C) Has no restriction sites (D) Is palindromic

Description : Chimeric DNA (A) Is found in bacteriophages (B) Contains unrelated genes (C) Has no restriction sites (D) Is palindromic

Last Answer : Answer : B

During replication, the template DNA is unwound (A) At one of the ends (B) At both the ends (C) At multiple sites (D) Nowhere

Description : During replication, the template DNA is unwound (A) At one of the ends (B) At both the ends (C) At multiple sites (D) Nowhere

Last Answer : Answer : C

The binding of prokaryotic DNA dependent RNA polymerase to promoter sites of genes is inhibited by the antibiotic: (A) Puromycin (B) Rifamycin (C) Terramycin (D) Streptomycin

Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sites of genes is inhibited by the antibiotic: (A) Puromycin (B) Rifamycin (C) Terramycin (D) Streptomycin

Last Answer : Answer : B

Tetracylin prevents synthesis of polypeptide by (A) Blocking mRNA formation from DNA (B) Releasing peptides from mRNA-tRNA complex (C) Competing with mRNA for ribosomal binding sites (D) Preventing binding of aminoacyl tRNA

Description : Tetracylin prevents synthesis of polypeptide by (A) Blocking mRNA formation from DNA (B) Releasing peptides from mRNA-tRNA complex (C) Competing with mRNA for ribosomal binding sites (D) Preventing binding of aminoacyl tRNA

Last Answer : Answer : D

.Balbiani rings (puffs) are sites of (a) DNA replication (b) RNA and protein synthesis (c) synthesis of polysaccharides (d) synthesis of lipids.

Description : .Balbiani rings (puffs) are sites of (a) DNA replication (b) RNA and protein synthesis (c) synthesis of polysaccharides (d) synthesis of lipids.

Last Answer : (b) RNA and protein synthesis

How does the restriction enzyme prevent the cutting of the endogenous DNA? -Biology

Description : How does the restriction enzyme prevent the cutting of the endogenous DNA? -Biology

Last Answer : answer:

How does the restriction enzyme prevent the cutting of the endogenous DNA? -Biology

Description : How does the restriction enzyme prevent the cutting of the endogenous DNA? -Biology

Last Answer : answer:

Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false

What is the role of the enzyme DNA primase?

Description : What is the role of the enzyme DNA primase?

Last Answer : Primase is an enzyme that synthesizes short RNA sequences called primers which serve as a starting point for DNA synthesis. Since primase produces RNA molecules, the enzyme is a type of RNA ... strand of nucleotides. Therefore, primase serves to prime and lay a foundation for DNA synthesis.

In which organism did the reverse transcriptase enzyme which performs DNA synthesis from RNA templates first appear?

Description : In which organism did the reverse transcriptase enzyme which performs DNA synthesis from RNA templates first appear?

Last Answer : Need answer

In the biosynthesis of c-DNA, the joining enzyme ligase requires (A) GTP (B) ATP (C) CTP (D) UTP

Description : In the biosynthesis of c-DNA, the joining enzyme ligase requires (A) GTP (B) ATP (C) CTP (D) UTP

Last Answer : Answer : B

Translocase is an enzyme required in the process of (A) DNA replication (B) RNA synthesis (C) Initiation of protein synthesis (D) Elongation of peptides

Description : Translocase is an enzyme required in the process of (A) DNA replication (B) RNA synthesis (C) Initiation of protein synthesis (D) Elongation of peptides

Last Answer : Answer : D

Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these

Description : Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these

Last Answer : Answer : A

RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III

Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III

Last Answer : Answer : B

The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers

Description : The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers

Last Answer : Answer : B

In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase

Description : In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase

Last Answer : Answer : B

Carbonic anhydrase enzyme has maximum turn over number (36 million). Minimum turn over number for an enzyme: (A) DNA polymerase (B) Lysozyme (C) Penicillase (D) Lactase dehydrogenase

Description : Carbonic anhydrase enzyme has maximum turn over number (36 million). Minimum turn over number for an enzyme: (A) DNA polymerase (B) Lysozyme (C) Penicillase (D) Lactase dehydrogenase

Last Answer : Answer : B

Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease

Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease

Last Answer : Answer : D

Which one of the following statements concerning glucose-6-phosphate dehydrogenase deficiency is correct? (A) Young R.B.Cs, particularly reticulocytes, contain the highest enzyme activity cells show less enzyme activity (B) Glucose-6-P Dehydroglucose deficiency leads to disfuction of many tissues (C) G-6-p Dehydroglucose deficiency is due to a single deletion of a large sequence of DNA in the G-6-PD gene (D) G-6-PD deficiency is precipitated by ingestion of drugs such as aspirin

Description : Which one of the following statements concerning glucose-6-phosphate dehydrogenase deficiency is correct? (A) Young R.B.Cs, particularly reticulocytes, contain the highest enzyme activity cells show less enzyme ... PD gene (D) G-6-PD deficiency is precipitated by ingestion of drugs such as aspirin

Last Answer : Answer : A

Consider the following statements: (1) Copper is present in cytochrome oxidane (2) Pantothenic acid is precursor of co-enzyme-A (3) Thiamine prohosphate is the prosthetic group in decarboxylases (4) Zinc is present in RNA and DNA polymerases

Description : Consider the following statements: (1) Copper is present in cytochrome oxidane (2) Pantothenic acid is precursor of co-enzyme-A (3) Thiamine prohosphate is the prosthetic group in decarboxylases (4) Zinc is present in RNA and DNA polymerases

Last Answer : Ans. ((b))

The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for both antigen and antibody detection b. Southern, northern, and western immunoblot techniques are used to detect DNA, RNA, or proteins, respectively c. Polymerase chain reaction (PCR) is a sensitive assay used to detect small amounts of microbial DNA, thus detecting infection at its early stages d. Infectious agents currently detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix of DNA by an enzyme known as DNA polymerase b. The initial product of DNA transcription is called heterogeneous nuclear RNA which codes directly for proteins c. After processing is complete, the mRNA is exported from the nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete

Which of the following statements are true of oxidants? A. In addition to their pathophysiologic roles in inflammation, injury, and infection, oxidants also have physiologic roles. B. Oxidants may be generated from activated neutrophils and during reperfusion following a period of ischemia. C. The deleterious effects of oxidants include lipid peroxidation and cell membrane damage, oxidative damage to DNA, and inhibition of adenosine triphosphate (ATP) synthesis. D. The mechanism of ischemia-reperfusion injury involved the catalytic production of superoxide anion (O 2•) by the enzyme xanthine oxidase.

Description : Which of the following statements are true of oxidants? A. In addition to their pathophysiologic roles in inflammation, injury, and infection, oxidants also have physiologic roles. B. Oxidants may ... involved the catalytic production of superoxide anion (O 2 ) by the enzyme xanthine oxidase.

Last Answer : Answer: ABCD DISCUSSION: Oxidants are reactive oxygen metabolites that have both physiologic and pathophysiologic roles. As potent oxidizing agents, oxidants are involved in cytochrome P ... leukocyte infiltration and activation, causing further tissue damage by the release of cytotoxic proteases

Consider the following biomolecules: 1. Vitamin A 2. Enzyme 3. Testosterone 4. DNA The biomolecules that are made in the human body are: (a) 1, 2 and 4 only (b) 1, 3 and 4 only (c) 2, 3 and 4 only (d) 1, 2 and 3 only

Description : Consider the following biomolecules: 1. Vitamin A 2. Enzyme 3. Testosterone 4. DNA The biomolecules that are made in the human body are: (a) 1, 2 and 4 only (b) 1, 3 and 4 only (c) 2, 3 and 4 only (d) 1, 2 and 3 only

Last Answer : Ans:(c)

The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNA polymerase

Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNA polymerase

Last Answer : (3) Helicase Explanation: A type of helicase enzyme called DNA helicase catalyzes the unwinding of the DNA helix at the time of replication. It uses the energy released through hydrolysis of ATP ( ... The enzyme is needed to separate the two strands of DNA also during transcription and DNA repair.

What characteristics of DNA polymerase III make it an ideal enzyme for replicating the whole genome?

Description : What characteristics of DNA polymerase III make it an ideal enzyme for replicating the whole genome?

Last Answer : The key features of DNA polymerase III are its catalytic potency, its fidelity, and its processivity. It is able to catalyze the addition of ~103 bases per second, compared to only about 10 per ... to dissociate from the template strand after synthesis of short (tens of bases) stretches of DNA.  

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)

Description : Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)

Last Answer : (c)

Which one of the following is wrongly matched? (a) Transcription - Writing information from DNA to tRNA. (b) Translation - Using information in mRNA to make protein. (c) Repressor protein - Binds to operator to stop enzyme synthesis. (d) Operon - Structural genes, operator and promoter.

Description : Which one of the following is wrongly matched? (a) Transcription - Writing information from DNA to tRNA. (b) Translation - Using information in mRNA to make protein. (c) Repressor protein - Binds to operator to stop enzyme synthesis. (d) Operon - Structural genes, operator and promoter.

Last Answer : (c) Repressor protein - Binds to operator to stop enzyme synthesis.

Telomerase is an enzyme which is a (a) simple protein (b) RNA (c) ribonucleoprotein (d) repetitive DNA

Description : Telomerase is an enzyme which is a (a) simple protein (b) RNA (c) ribonucleoprotein (d) repetitive DNA

Last Answer : (c) ribonucleoprotein

Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase

Description : Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase

Last Answer : b) DNA helicase

Which one of the following is wrongly matched? (a) Transcription - Writing information from DNA to tRNA. (b) Translation - Using information in mRNA to make protein. (c) Repressor protein - Binds to operator to stop enzyme synthesis. (d) Operon - Structural genes, operator and promoter.

Description : Which one of the following is wrongly matched? (a) Transcription - Writing information from DNA to tRNA. (b) Translation - Using information in mRNA to make protein. (c) Repressor protein - Binds to operator to stop enzyme synthesis. (d) Operon - Structural genes, operator and promoter.

Last Answer : (d) Operon - Structural genes, operator and promoter

Telomerase is an enzyme which is a (a) simple protein (b) RNA (c) ribonucleoprotein (d) repetitive DNA

Description : Telomerase is an enzyme which is a (a) simple protein (b) RNA (c) ribonucleoprotein (d) repetitive DNA

Last Answer : (c) ribonucleoprotein

The figure below shows the structure of a mitochondrion with its four parts labelled A, B, C and D. Select the part correctly matched with its function. D B A C (a) D (outer membrane) – gives rise to inner membrane by splitting (b) B (inner membrane) – forms infoldings called cristae (c) C (crista) – possesses single circular DNA molecule and ribosomes (d) A (matrix) – major site for respiratory chain enzyme

Description : The figure below shows the structure of a mitochondrion with its four parts labelled A, B, C and D. Select the part correctly matched with its function. D B A C (a) D (outer ... possesses single circular DNA molecule and ribosomes (d) A (matrix) - major site for respiratory chain enzyme

Last Answer : (b) B (inner membrane) – forms infoldings called cristae

The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase

Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase

Last Answer : d. RNA polymerase

Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease

Last Answer : a) Topoisomease II

The antiviral action of amantadine is exerted through: A. Interaction with the viral M2 protein B. Interaction with a virus directed thymidine kinase C. Inhibition of a viral protease enzyme D. Inhibition of viral RNA mediated DNA synthesi

Description : The antiviral action of amantadine is exerted through: A. Interaction with the viral M2 protein B. Interaction with a virus directed thymidine kinase C. Inhibition of a viral protease enzyme D. Inhibition of viral RNA mediated DNA synthesi

Last Answer : A. Interaction with the viral M2 protein

The polyene antibiotics act by: A. Inhibiting fungal cytochrome P450 enzyme B. Binding to ergosterol and creating micropores in fungal cell membrane C. Inhibiting fungal DNA synthesis D. Disorienting microtubules in fungal cells

Description : The polyene antibiotics act by: A. Inhibiting fungal cytochrome P450 enzyme B. Binding to ergosterol and creating micropores in fungal cell membrane C. Inhibiting fungal DNA synthesis D. Disorienting microtubules in fungal cells

Last Answer : B. Binding to ergosterol and creating micropores in fungal cell membrane

Indicate the enzyme(s) inhibited by fluoroquinolones: A. Both 'A' and 'C' B. Topoisomerase II C. Topoisomerase IV D. DNA gyrase

Description : Indicate the enzyme(s) inhibited by fluoroquinolones: A. Both 'A' and 'C' B. Topoisomerase II C. Topoisomerase IV D. DNA gyrase

Last Answer : D. DNA gyrase

6. Which of the following DNA sequences would a Restriction enzyme recognize and cut? a. ATGCAC TACGTG b. GATATC CTATAG c. TAGATA ATCTAT d. AATATA TTATAT

Description : 6. Which of the following DNA sequences would a Restriction enzyme recognize and cut? a. ATGCAC TACGTG b. GATATC CTATAG c. TAGATA ATCTAT d. AATATA TTATAT

Last Answer : b. GATATC CTATAG