Give the method of preparation of “Small pox vaccine” using egg.

Give the method of preparation of “Small pox vaccine” using egg.
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1 Answer

Answer :

Small pox vaccine is prepared by two methods 1) By using animals 2) By using Eggs

By using eggs:  

Hen egg is used

(Which is incubated after 12 days)

↓

Small cut on the shell

(exposed chorio-allantoic membrane)

↓

In this membrane, viruses are inoculated

(by seed of known potency )

↓

Cut was sealed by flap or paraffin wax

↓

Again incubate for 72 hours

↓

Using aseptic condition, shell is removed and chorio-allantoic membrane is

separated

↓

Contents are added in normal saline solution at 0° C

↓

Add 50 % glycerin

↓

Material is ground to produce homogenized suspension.

↓

Transfer to suitable sterile container and freeze dried

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