Define the term vaccine. Discuss the method of preparation of small pox vaccine using animals

Define the term vaccine. Discuss the method of preparation of small pox vaccine using animals
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1 Answer

Answer :

Definition:  

Vaccines are antigenic preparations which stimulate antibody formation and producing immunity.

Small pox vaccine is prepared by two methods

1) By using animals

2) By using Eggs By using eggs:

1) By using animals:  

Animal: calves or Sheep.

Selection of animal: healthy, non-diseased, animal kept for 10 to 14 days under observation.

Scarification: Abdominal part & flanks parts shaved and disinfected.

Inoculation: light incision made in the cleared skin without drawing blood with scarifies. Then area is rubbed with some seeds vaccine of known potency

Incubation: 7-9 days, pustule formed at lining.

Collection of virus: Animal operated and killed, the material in pustules is withdrawn in aseptic condition.

Purification: pustules + glycerine mixed and stored at -100C to remove impurities.

Filling sealing and storage: filled in final container under aseptic condition and freeze drying. 
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