1. BCG vaccine: It is freeze‐ dried preparation containing live culture of the bacillus Calmette and Guerin strain of Mycobacterium tuberculosis.
Method of preparation : The bacilli are grown on a suitable culture media until 1 mg when plated out on a suitable solid culture media shows not less than 20 million colonies. The growth period Should not be more than 14 days in any case. After a suitable growth, they are separated by filtration in the form of a cake. The cake is homogenized in a grinding flask and suspended in a suitable sterile liquid medium designed to preserve the antigenicity and viability of the vaccine. The suspension is transferred into the Final sterile containers and freeze‐dried. Then containers are sealed so as to prevent Contamination or deterioration of the vaccine. The vaccine contains no antimicrobial agent.
Storage: Store in hermetically sealed light resistant glass containers at a temperature Between 20 C and 80 C. The reconstituted vaccine should be used immediately after its preparation.
Uses: Immunizing agent which provides protection against tuberculosis.
2. Silverson Mixer Homogenizer
Construction:
1) It consists of emulsified head which is covered with fine meshed stainless steel sieves.
2) Emulsifier head consist of number of blades which rotate at very high speed to produce powerful sharing action.
3) Blades are rotate by using an electric motor fitted at the top.
Working:
1) Emulsifier head is placed in the vessel containing immiscible liquid in such a way that it should get dipped into it.
2) When the motor is started liquid is sucked through fine holes and oil is reduced into fine globules due to the rotation of blades.
3) So fine emulsion is produce which is then expelled out.
Use:
Useful for the preparation of fine emulsion and suspension.
Diagram: