Description : The mammalian DNA polymerase involved in error correction is (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase δ
Last Answer : Answer : B
Description : α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase
Last Answer : Answer : C
Description : Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ
Description : Reverse transcriptase activity is present in the eukaryotic: (A) DNA polymerase α (B) DNA polymerase γ (C) Telomerase (D) DNA polymerase II
Description : Mammalian DNA polymerase γ is located in (A) Nucleus (B) Nucleolus (C) Mitochondria (D) Cytosol
Description : Replication of nuclear DNA in mammals is catalysed by (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase III
Last Answer : Answer : A
Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase
Description : Mammalian RNA polymerase III synthesises (A) rRNA (B) mRNA (C) tRNA (D) hnRNA
Description : Mammalian RNA polymerase I synthesises (A) mRNA (B) rRNA (C) tRNA (D) hnRNA
Description : Match the organism with its use in biotechnology. (A) Bacillus (i) Cloning vector thuringiensis (B) Thermus (ii) Construction of first aquaticus rDNA molecule (C) Agrobacterium (iii)DNA polymerase tumefaciens (D) Salmonella (iv) Cry proteins ... (ii) (c) (iii) (ii) (iv) (i) (d) (iii) (iv) (i) (ii)
Last Answer : (b) (iv) (iii) (i) (ii)
Description : An example of α-amino acid not present in proteins but essential in mammalian metabolism is (A) 3-Amino 3-hydroxypropanoic acid (B) 2-Amino 3-hydroxybutanoic acid (C) 2-Amino 4-mercaptobutanoic acid (D) 2-Amino 3-mercaptopropanoic acid
Description : In mammals, synthesis of mRNA is catalysed by (A) RNA polymerase I (B) RNA polymerase II (C) RNA polymerase III(D) RNA polymerase IV
Description : Ciprofloxacin inhibits (A) DNA topisomerase II (B) DNA polymerase I (C) DNA polymerase III (D) DNA gyrase
Last Answer : Answer : D
Description : A consensus sequence on DNA, called TATA box, is the site for attachment of (A) RNA-dependent DNA polymerase (B) DNA-dependent RNA polymerase (C) DNA-dependent DNA polymerase (D) DNA topoisomerase II
Description : DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II
Description : Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Description : Deoxyribonucleotides are added to RNA primer by (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Description : In mammalian cell cycle, synthesis of DNA occurs during (A) S phase (B) G1 phase (C) Mitotic Phase (D) G2 phase
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes
Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin
Description : Another name for reverse transcriptase is (A) DNA dependent DNA polymerase (B) DNA dependent RNA polymerase (C) RNA dependent DNA polymerase (D) RNA dependent RNA polymerase
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes
Description : RNA directed DNA polymerase is (A) Replicase (B) Transcriptase (C) Reversetranscriptase (D) Polymerase–III
Description : DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III
Description : The antibiotic which inhibits DNA dependent RNA polymerase is (A) Mitomycin C (B) Actinomycin d (C) Streptomycin (D) Puromycin
Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase
Description : Optimum temperature of DNA polymerase of T. aquaticus is (A) 30°C (B) 37°C (C) 54°C (D) 72°C
Description : The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes
Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases
Description : Myeloma cells and lymphocytes can be fused by using (A) Calcium chloride (B) Ethidium bromide (C) Polyethylene glycol (D) DNA polymerase
Description : Xeroderma pigmentosum results from a defect in (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Description : Damage to DNA caused by ultraviolet radiation can be repaired by (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Description : The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Description : The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Description : DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these
Description : DNA-dependent RNA polymerase is a (A) Monomer (B) Dimer (C) Trimer (D) Tetramer
Description : All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses
Description : 3’→5’ Exonuclease activity of DNA polymerase I (A) Removes ribonucleotides (B) Adds deoxyribonucleotides (C) Corrects errors in replication (D) Hydrolyses DNA into mononucleotides
Description : DNA polymerase I possesses (A) Polymerase activity (B) 3’→5’ Exonuclease activity (C) 5’→3’ Exonuclease activity (D) All of these
Description : DNA polymerase III holoenzyme possesses (A) Polymerase activity (B) 3’→5’ Exonuclease activity (C) 5’→3’ Exonuclease and polymerase activities (D) 3’→5’ Exonuclease and polymerase activities
Description : Negative supercoils are introduced in DNA by (A) Helicase (B) DNA ligase (C) DNA gyrase (D) DNA polymerase III holoenzyme
Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III
Description : The region of DNA known as TATA BOX is the site for binding of (A) DNA polymerase (B) DNA topoisomerase (C) DNA dependent RNA polymerase (D) Polynucleotide phosphorylase
Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sites of genes is inhibited by the antibiotic: (A) Puromycin (B) Rifamycin (C) Terramycin (D) Streptomycin
Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain
Description : In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase
Description : Which one of the following statements correctly describes eukaryotic DNA? (A) If uses DNA polymerase with nuclease activities (B) It is replicated bidirectionally at many points (C) It contains no repetitive DNA (D) It is nonlinear
Description : Carbonic anhydrase enzyme has maximum turn over number (36 million). Minimum turn over number for an enzyme: (A) DNA polymerase (B) Lysozyme (C) Penicillase (D) Lactase dehydrogenase
Description : A 20-carbon fatty acid among the following is (A) Linoleic acid (B) α -Linolenic acid (C) β -Linolenic acid (D) Arachidonic acid